ID   YUC9_ARATH              Reviewed;         421 AA.
AC   O64489;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 144.
DE   RecName: Full=Probable indole-3-pyruvate monooxygenase YUCCA9;
DE            EC=1.14.13.168;
DE   AltName: Full=Flavin-containing monooxygenase YUCCA9;
GN   Name=YUC9; Synonyms=YUCCA9; OrderedLocusNames=At1g04180; ORFNames=F20D22.5;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=16818609; DOI=10.1101/gad.1415106;
RA   Cheng Y., Dai X., Zhao Y.;
RT   "Auxin biosynthesis by the YUCCA flavin monooxygenases controls the
RT   formation of floral organs and vascular tissues in Arabidopsis.";
RL   Genes Dev. 20:1790-1799(2006).
RN   [4]
RP   GENE FAMILY.
RC   STRAIN=cv. Columbia;
RX   PubMed=17461789; DOI=10.1111/j.1365-313x.2007.03101.x;
RA   Hansen B.G., Kliebenstein D.J., Halkier B.A.;
RT   "Identification of a flavin-monooxygenase as the S-oxygenating enzyme in
RT   aliphatic glucosinolate biosynthesis in Arabidopsis.";
RL   Plant J. 50:902-910(2007).
RN   [5]
RP   INDUCTION.
RX   PubMed=22109847; DOI=10.1007/s00425-011-1552-3;
RA   Lee M., Jung J.H., Han D.Y., Seo P.J., Park W.J., Park C.M.;
RT   "Activation of a flavin monooxygenase gene YUCCA7 enhances drought
RT   resistance in Arabidopsis.";
RL   Planta 235:923-938(2012).
CC   -!- FUNCTION: Involved in auxin biosynthesis. Belongs to the set of
CC       redundant YUCCA genes probably responsible for auxin biosynthesis in
CC       roots.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + indole-3-pyruvate + NADPH + O2 = (indol-3-yl)acetate +
CC         CO2 + H2O + NADP(+); Xref=Rhea:RHEA:34331, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:17640, ChEBI:CHEBI:30854, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.14.13.168;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- INDUCTION: Up-regulated by drought. {ECO:0000269|PubMed:22109847}.
CC   -!- SIMILARITY: Belongs to the FMO family. {ECO:0000305}.
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DR   EMBL; AC002411; AAC16744.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE27667.1; -; Genomic_DNA.
DR   PIR; T00955; T00955.
DR   RefSeq; NP_171914.1; NM_100299.2.
DR   AlphaFoldDB; O64489; -.
DR   SMR; O64489; -.
DR   STRING; 3702.AT1G04180.1; -.
DR   PaxDb; O64489; -.
DR   PRIDE; O64489; -.
DR   EnsemblPlants; AT1G04180.1; AT1G04180.1; AT1G04180.
DR   GeneID; 837024; -.
DR   Gramene; AT1G04180.1; AT1G04180.1; AT1G04180.
DR   KEGG; ath:AT1G04180; -.
DR   Araport; AT1G04180; -.
DR   TAIR; locus:2020250; AT1G04180.
DR   eggNOG; KOG1399; Eukaryota.
DR   HOGENOM; CLU_006909_2_0_1; -.
DR   InParanoid; O64489; -.
DR   OMA; LHECEFF; -.
DR   OrthoDB; 405736at2759; -.
DR   PhylomeDB; O64489; -.
DR   UniPathway; UPA00151; -.
DR   PRO; PR:O64489; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; O64489; baseline and differential.
DR   Genevisible; O64489; AT.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0103075; F:indole-3-pyruvate monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR   GO; GO:0004499; F:N,N-dimethylaniline monooxygenase activity; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0009851; P:auxin biosynthetic process; IBA:GO_Central.
DR   GO; GO:0009723; P:response to ethylene; IEP:TAIR.
DR   GO; GO:0009635; P:response to herbicide; IEP:TAIR.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR020946; Flavin_mOase-like.
DR   Pfam; PF00743; FMO-like; 1.
DR   SUPFAM; SSF51905; SSF51905; 2.
PE   2: Evidence at transcript level;
KW   Auxin biosynthesis; FAD; Flavoprotein; Monooxygenase; NADP; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..421
FT                   /note="Probable indole-3-pyruvate monooxygenase YUCCA9"
FT                   /id="PRO_0000400076"
FT   BINDING         29..34
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   BINDING         196..201
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   421 AA;  47402 MW;  F616FF3E1987CF79 CRC64;
     MENMFRLMAS EEYFSERRCV WVNGPVIVGA GPSGLATAAC LHDQGVPFVV VERSDCIASL
     WQKRTYDRLK LHLPKKFCQL PKMPFPDHYP EYPTKRQFID YLESYANRFD IKPEFNKSVE
     SARFDETSGL WRVRTTSDGE EMEYICRWLV VATGENAERV VPEINGLMTE FDGEVIHACE
     YKSGEKFRGK RVLVVGCGNS GMEVSLDLAN HNAITSMVVR SSVHVLPREI MGKSTFGISV
     MMMKWLPLWL VDKLLLILSW LVLGSLSNYG LKRPDIGPME LKSMTGKTPV LDIGALEKIK
     SGDVEIVPAI KQFSRHHVEL VDGQKLDIDA VVLATGYRSN VPSWLQESEF FSKNGFPKSP
     FPNAWKGKSG LYAAGFTRKG LAGASVDAVN IAQDIGNVWR EETKRQKMRR NVGHRRCISV
     A