YUKE_BACSU
ID YUKE_BACSU Reviewed; 97 AA.
AC C0SP85; P71072; Q795L2;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Protein YukE {ECO:0000305};
GN Name=yukE; OrderedLocusNames=BSU31910;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9168598; DOI=10.1099/00221287-143-5-1489;
RA Oudega B., Vandenbol M., Koningstein G.;
RT "A 12kb nucleotide sequence containing the alanine dehydrogenase gene at
RT 279 degree on the Bacillus subtilis chromosome.";
RL Microbiology 143:1489-1491(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=15576783; DOI=10.1128/jb.186.24.8337-8346.2004;
RA Sao-Jose C., Baptista C., Santos M.A.;
RT "Bacillus subtilis operon encoding a membrane receptor for bacteriophage
RT SPP1.";
RL J. Bacteriol. 186:8337-8346(2004).
RN [5]
RP SUBUNIT, SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=ATCC 6051;
RX PubMed=23861817; DOI=10.1371/journal.pone.0067840;
RA Baptista C., Barreto H.C., Sao-Jose C.;
RT "High levels of DegU-P activate an Esat-6-like secretion system in Bacillus
RT subtilis.";
RL PLoS ONE 8:E67840-E67840(2013).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=168 / PY79;
RX PubMed=24798022; DOI=10.1371/journal.pone.0096267;
RA Huppert L.A., Ramsdell T.L., Chase M.R., Sarracino D.A., Fortune S.M.,
RA Burton B.M.;
RT "The ESX system in Bacillus subtilis mediates protein secretion.";
RL PLoS ONE 9:E96267-E96267(2014).
RN [7]
RP SUBUNIT, SUBCELLULAR LOCATION, DOMAIN, AND MUTAGENESIS OF TRP-44; GLU-45
RP AND GLY-46.
RX PubMed=24828531; DOI=10.1073/pnas.1322200111;
RA Sysoeva T.A., Zepeda-Rivera M.A., Huppert L.A., Burton B.M.;
RT "Dimer recognition and secretion by the ESX secretion system in Bacillus
RT subtilis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:7653-7658(2014).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=34280190; DOI=10.1371/journal.pgen.1009682;
RA Kobayashi K.;
RT "Diverse LXG toxin and antitoxin systems specifically mediate intraspecies
RT competition in Bacillus subtilis biofilms.";
RL PLoS Genet. 17:e1009682-e1009682(2021).
CC -!- FUNCTION: Required to deliver LXG toxins to target cells.
CC {ECO:0000269|PubMed:34280190}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:23861817,
CC ECO:0000269|PubMed:24828531}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23861817,
CC ECO:0000269|PubMed:24798022, ECO:0000269|PubMed:24828531}.
CC Note=Secreted via the ESX/ESAT-6-like secretion system (BsEss) / type
CC VII secretion system (T7SS) (PubMed:23861817, PubMed:24798022). Can be
CC secreted as a dimer (PubMed:24828531). {ECO:0000269|PubMed:23861817,
CC ECO:0000269|PubMed:24798022, ECO:0000269|PubMed:24828531}.
CC -!- INDUCTION: Positively regulated by phosphorylated DegU.
CC {ECO:0000269|PubMed:23861817}.
CC -!- DOMAIN: The WXG motif and the C-terminal tail are crucial for
CC secretion. {ECO:0000269|PubMed:24828531}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene display an increased
CC bacteriophage SPP1 resistance phenotype (PubMed:15576783). Loss of
CC delivery of LXG type toxins to target cells (PubMed:34280190).
CC {ECO:0000269|PubMed:15576783, ECO:0000269|PubMed:34280190}.
CC -!- SIMILARITY: Belongs to the WXG100 family. sagEsxA-like subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB04773.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB04773.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z82015; CAB04773.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL009126; CAB15179.2; -; Genomic_DNA.
DR PIR; G70013; G70013.
DR RefSeq; NP_391069.2; NC_000964.3.
DR RefSeq; WP_003220661.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; C0SP85; -.
DR SMR; C0SP85; -.
DR STRING; 224308.BSU31910; -.
DR jPOST; C0SP85; -.
DR PaxDb; C0SP85; -.
DR PRIDE; C0SP85; -.
DR EnsemblBacteria; CAB15179; CAB15179; BSU_31910.
DR GeneID; 50136270; -.
DR GeneID; 64304936; -.
DR GeneID; 936578; -.
DR KEGG; bsu:BSU31910; -.
DR PATRIC; fig|224308.179.peg.3456; -.
DR eggNOG; COG4842; Bacteria.
DR OMA; SDLKGMW; -.
DR PhylomeDB; C0SP85; -.
DR BioCyc; BSUB:BSU31910-MON; -.
DR PRO; PR:C0SP85; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR036689; ESAT-6-like_sf.
DR InterPro; IPR010310; T7SS_ESAT-6-like.
DR Pfam; PF06013; WXG100; 1.
DR SUPFAM; SSF140453; SSF140453; 1.
DR TIGRFAMs; TIGR03930; WXG100_ESAT6; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Reference proteome; Secreted.
FT CHAIN 1..97
FT /note="Protein YukE"
FT /id="PRO_0000383635"
FT COILED 21..94
FT /evidence="ECO:0000255"
FT MUTAGEN 44
FT /note="W->A: Lack of secretion, but does not affect
FT dimerization."
FT /evidence="ECO:0000269|PubMed:24828531"
FT MUTAGEN 44
FT /note="W->E,G,P,R: Lack of secretion."
FT /evidence="ECO:0000269|PubMed:24828531"
FT MUTAGEN 44
FT /note="W->F,Y: Strong decrease in secretion."
FT /evidence="ECO:0000269|PubMed:24828531"
FT MUTAGEN 45
FT /note="E->A: Does not affect secretion and dimerization."
FT /evidence="ECO:0000269|PubMed:24828531"
FT MUTAGEN 45
FT /note="E->D,K,Q,R: Does not affect secretion."
FT /evidence="ECO:0000269|PubMed:24828531"
FT MUTAGEN 45
FT /note="E->G: Strong decrease in secretion."
FT /evidence="ECO:0000269|PubMed:24828531"
FT MUTAGEN 46
FT /note="G->A: Lack of secretion, but does not affect
FT dimerization."
FT /evidence="ECO:0000269|PubMed:24828531"
FT MUTAGEN 46
FT /note="G->P,R: Lack of secretion."
FT /evidence="ECO:0000269|PubMed:24828531"
SQ SEQUENCE 97 AA; 10993 MW; C36A9624626BA4C3 CRC64;
MAGLIRVTPE ELRAMAKQYG VESQEVLNQV DRLNRMISDL KSMWEGASSE AFADQYEQLK
PSFIKMSDLL QDVNQQLDQT ANTLESTDQD IANQIRG