CC033_HUMAN
ID CC033_HUMAN Reviewed; 294 AA.
AC Q6P1S2; A8K1H5; Q86YE6; Q8IXA7; Q96NB5;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein C3orf33;
DE AltName: Full=Protein AC3-33;
GN Name=C3orf33; ORFNames=MSTP052;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP ASN-160.
RC TISSUE=Corpus callosum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ASN-160.
RC TISSUE=Cervix, and Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-294 (ISOFORM 1), AND VARIANT
RP ASN-160.
RC TISSUE=Heart;
RA Zhao B., Xu Y.Y., Liu Y.Q., Wang X.Y., Liu B., Ye J., Song L., Zhao Y.,
RA Cao H.Q., Zhao X.W., Gao Y., Liu L.S., Ding J.F., Gao R.L., Wu Q.Y.,
RA Qiang B.Q., Yuan J.G., Liew C.C., Zhao M.S., Hui R.T.;
RL Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [7]
RP FUNCTION (ISOFORM 2), SUBCELLULAR LOCATION (ISOFORM 2), AND TISSUE
RP SPECIFICITY.
RX PubMed=20680465; DOI=10.1007/s11033-010-0240-x;
RA Hao D., Gao P., Liu P., Zhao J., Wang Y., Yang W., Lu Y., Shi T., Zhang X.;
RT "AC3-33, a novel secretory protein, inhibits Elk1 transcriptional activity
RT via ERK pathway.";
RL Mol. Biol. Rep. 38:1375-1382(2011).
CC -!- FUNCTION: [Isoform 2]: Secreted protein may play a role in
CC transcription regulation via the MAPK3/MAPK1 pathway through an
CC unidentified receptor on the plasma membrane.
CC -!- INTERACTION:
CC Q6P1S2; Q53QW1: TEX44; NbExp=3; IntAct=EBI-13382270, EBI-10278496;
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Secreted
CC {ECO:0000269|PubMed:20680465}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6P1S2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P1S2-2; Sequence=VSP_046233;
CC -!- TISSUE SPECIFICITY: Highly expressed in ileocecal tissue and
CC endometrium. {ECO:0000269|PubMed:20680465}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO06952.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK055701; BAB70989.1; -; mRNA.
DR EMBL; AK289890; BAF82579.1; -; mRNA.
DR EMBL; AC104472; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471052; EAW78746.1; -; Genomic_DNA.
DR EMBL; BC042198; AAH42198.1; -; mRNA.
DR EMBL; BC064898; AAH64898.1; -; mRNA.
DR EMBL; AF115515; AAO06952.1; ALT_INIT; mRNA.
DR CCDS; CCDS54659.1; -. [Q6P1S2-2]
DR CCDS; CCDS77843.1; -. [Q6P1S2-1]
DR RefSeq; NP_001295158.1; NM_001308229.1. [Q6P1S2-1]
DR RefSeq; NP_775928.1; NM_173657.2. [Q6P1S2-2]
DR RefSeq; XP_005247436.1; XM_005247379.4. [Q6P1S2-2]
DR RefSeq; XP_011511013.1; XM_011512711.2. [Q6P1S2-2]
DR AlphaFoldDB; Q6P1S2; -.
DR BioGRID; 130076; 30.
DR IntAct; Q6P1S2; 7.
DR STRING; 9606.ENSP00000445446; -.
DR iPTMnet; Q6P1S2; -.
DR PhosphoSitePlus; Q6P1S2; -.
DR BioMuta; C3orf33; -.
DR DMDM; 317373335; -.
DR EPD; Q6P1S2; -.
DR MassIVE; Q6P1S2; -.
DR MaxQB; Q6P1S2; -.
DR PaxDb; Q6P1S2; -.
DR PeptideAtlas; Q6P1S2; -.
DR PRIDE; Q6P1S2; -.
DR ProteomicsDB; 66873; -. [Q6P1S2-1]
DR ProteomicsDB; 77500; -.
DR Antibodypedia; 46752; 54 antibodies from 12 providers.
DR DNASU; 285315; -.
DR Ensembl; ENST00000340171.7; ENSP00000342512.2; ENSG00000174928.16. [Q6P1S2-1]
DR Ensembl; ENST00000534941.2; ENSP00000445446.1; ENSG00000174928.16. [Q6P1S2-2]
DR GeneID; 285315; -.
DR KEGG; hsa:285315; -.
DR MANE-Select; ENST00000340171.7; ENSP00000342512.2; NM_001308229.2; NP_001295158.1.
DR UCSC; uc003fam.2; human. [Q6P1S2-1]
DR CTD; 285315; -.
DR GeneCards; C3orf33; -.
DR HGNC; HGNC:26434; C3orf33.
DR HPA; ENSG00000174928; Low tissue specificity.
DR MIM; 619654; gene.
DR neXtProt; NX_Q6P1S2; -.
DR OpenTargets; ENSG00000174928; -.
DR PharmGKB; PA142672394; -.
DR VEuPathDB; HostDB:ENSG00000174928; -.
DR eggNOG; ENOG502QR9T; Eukaryota.
DR GeneTree; ENSGT00390000004493; -.
DR HOGENOM; CLU_069834_0_0_1; -.
DR InParanoid; Q6P1S2; -.
DR OMA; YWRIHRN; -.
DR OrthoDB; 1030642at2759; -.
DR PhylomeDB; Q6P1S2; -.
DR TreeFam; TF313180; -.
DR PathwayCommons; Q6P1S2; -.
DR SignaLink; Q6P1S2; -.
DR BioGRID-ORCS; 285315; 16 hits in 1044 CRISPR screens.
DR ChiTaRS; C3orf33; human.
DR GenomeRNAi; 285315; -.
DR Pharos; Q6P1S2; Tbio.
DR PRO; PR:Q6P1S2; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q6P1S2; protein.
DR Bgee; ENSG00000174928; Expressed in right adrenal gland and 96 other tissues.
DR ExpressionAtlas; Q6P1S2; baseline and differential.
DR Genevisible; Q6P1S2; HS.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; IDA:UniProtKB.
DR GO; GO:0051090; P:regulation of DNA-binding transcription factor activity; IDA:UniProtKB.
DR Gene3D; 2.40.50.90; -; 1.
DR InterPro; IPR042421; C3orf33-like.
DR InterPro; IPR035437; SNase_OB-fold_sf.
DR PANTHER; PTHR28434; PTHR28434; 1.
DR SUPFAM; SSF50199; SSF50199; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Membrane; Reference proteome; Secreted;
KW Transmembrane; Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:19413330"
FT CHAIN 2..294
FT /note="Protein C3orf33"
FT /id="PRO_0000254577"
FT TRANSMEM 40..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:19413330"
FT VAR_SEQ 1..43
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_046233"
FT VARIANT 47
FT /note="A -> T (in dbSNP:rs9853408)"
FT /id="VAR_028846"
FT VARIANT 160
FT /note="S -> N (in dbSNP:rs358733)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334, ECO:0000269|Ref.5"
FT /id="VAR_028847"
FT CONFLICT 143
FT /note="F -> V (in Ref. 5; AAO06952)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 294 AA; 33765 MW; 5B780859FE75268F CRC64;
MAGQPAATGS PSADKDGMEP NVVARISQWA DDHLRLVRNI STGMAIAGIM LLLRSIRLTS
KFTSSSDIPV EFIRRNVKLR GRLRRITENG LEIEHIPITL PIIASLRKEP RGALLVKLAG
VELAETGKAW LQKELKPSQL LWFQLLGKEN SALFCYLLVS KGGYFSVNLN EEILRRGLGK
TVLVKGLKYD SKIYWTVHRN LLKAELTALK KGEGIWKEDS EKESYLEKFK DSWREIWKKD
SFLKTTGSDF SLKKESYYEK LKRTYEIWKD NMNNCSLILK FRELISRINF RRKG
