ID   YWFF_LACLA              Reviewed;         513 AA.
AC   Q9CDP0;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Uncharacterized RNA methyltransferase YwfF;
DE            EC=2.1.1.-;
GN   Name=ywfF; OrderedLocusNames=LL2179; ORFNames=L37880;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; AE005176; AAK06277.1; -; Genomic_DNA.
DR   PIR; C86897; C86897.
DR   RefSeq; NP_268336.1; NC_002662.1.
DR   RefSeq; WP_010906363.1; NC_002662.1.
DR   AlphaFoldDB; Q9CDP0; -.
DR   SMR; Q9CDP0; -.
DR   STRING; 272623.L37880; -.
DR   PaxDb; Q9CDP0; -.
DR   EnsemblBacteria; AAK06277; AAK06277; L37880.
DR   KEGG; lla:L37880; -.
DR   PATRIC; fig|272623.7.peg.2339; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_014689_7_1_9; -.
DR   OMA; YCGVGGF; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 2.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..513
FT                   /note="Uncharacterized RNA methyltransferase YwfF"
FT                   /id="PRO_0000161988"
FT   DOMAIN          3..61
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        434
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         309
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         338
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         359
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         407
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   513 AA;  58919 MW;  B3F8695DAD9B8BEF CRC64;
     MINLKIGQKL QLEIERMGIN GEGIGVISGR LVFIPYALPG EEVLVEITEN ARNFSRAKLV
     KIIEKSPNRV KPQDRAYHEM SQSHIMHLSY PMQLEFKRDV MRQALEKYKP AGWRNYELRA
     TLGMENTLGY RNKLQFQVRR LNDGTVIAGL YQEGTHHLVN LDNCLVQDPK TQEIINHICR
     LIEKFDLPVY DERKIGGIRT VMVRRSQKTG EVQIIFVTST PIILDGQVWP ELREEPSKRQ
     KNSFVKFDKM LSALTEEFEE IVTVAVNFHP RKTSEIYGER TQILFNEKET ITEGVLDYEF
     ELSPRAFYQL NSEQANVLYG EAVKALNPKK DDRVIDAYCG VGTIGFAVAK KVKSVHGMDI
     TPESIFDARE NAKRLGLKNC HYEIGKAERI IPNWNKSGHR ATAMIVDPPR TGLDDALLET
     ITKFPPEKMV YVSCNVSTLA KDLVVLANFY KVEYIQSVDM FPHTARTEAV VKLTKRDEPL
     KIVKFEHLEE ERKAEAFAKG KKRGRRPQKY GKY