ID   YWOF_BACSU              Reviewed;         468 AA.
AC   P94576; Q795A4;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Uncharacterized protein YwoF;
DE   Flags: Precursor;
GN   Name=ywoF; OrderedLocusNames=BSU36460;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA   Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA   Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA   Kunst F., Danchin A., Glaser P.;
RT   "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT   degrees).";
RL   Microbiology 143:3313-3328(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=15266115; DOI=10.1023/b:bile.0000035481.03854.41;
RA   Cui Z.-L., Zhang X.-Z., Zhang Z.-H., Li S.-P.;
RT   "Construction and application of a promoter-trapping vector with methyl
RT   parathion hydrolase gene mpd as the reporter.";
RL   Biotechnol. Lett. 26:1115-1118(2004).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=16607952; DOI=10.1016/s1872-2075(06)60029-0;
RA   Zhang X.-Z., Yan X., Cui Z.-L., Li S.-P.;
RT   "Recombinant expression and secretion of mpd gene using the promoters and
RT   signal peptide-encoding sequences of ytkA and ywoF gene from Bacillus
RT   subtilis.";
RL   Sheng Wu Gong Cheng Xue Bao 22:249-256(2006).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:16607952}.
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DR   EMBL; Z82987; CAB05379.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15663.1; -; Genomic_DNA.
DR   PIR; A70065; A70065.
DR   RefSeq; NP_391527.1; NC_000964.3.
DR   RefSeq; WP_003243080.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P94576; -.
DR   SMR; P94576; -.
DR   STRING; 224308.BSU36460; -.
DR   CAZy; PL9; Polysaccharide Lyase Family 9.
DR   PaxDb; P94576; -.
DR   PRIDE; P94576; -.
DR   EnsemblBacteria; CAB15663; CAB15663; BSU_36460.
DR   GeneID; 936925; -.
DR   KEGG; bsu:BSU36460; -.
DR   PATRIC; fig|224308.179.peg.3945; -.
DR   eggNOG; COG3420; Bacteria.
DR   InParanoid; P94576; -.
DR   OMA; GIDLIGY; -.
DR   BioCyc; BSUB:BSU36460-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR039448; Beta_helix.
DR   InterPro; IPR006633; Carb-bd_sugar_hydrolysis-dom.
DR   InterPro; IPR006626; PbH1.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF13229; Beta_helix; 1.
DR   SMART; SM00722; CASH; 1.
DR   SMART; SM00710; PbH1; 8.
DR   SUPFAM; SSF51126; SSF51126; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..468
FT                   /note="Uncharacterized protein YwoF"
FT                   /id="PRO_0000359913"
FT   REPEAT          125..154
FT                   /note="PbH1 1"
FT   REPEAT          156..185
FT                   /note="PbH1 2"
FT   REPEAT          189..214
FT                   /note="PbH1 3"
FT   REPEAT          216..238
FT                   /note="PbH1 4"
FT   REPEAT          249..283
FT                   /note="PbH1 5"
FT   REPEAT          284..305
FT                   /note="PbH1 6"
FT   REPEAT          312..334
FT                   /note="PbH1 7"
FT   REPEAT          397..420
FT                   /note="PbH1 8"
SQ   SEQUENCE   468 AA;  51452 MW;  97FF0CA0B3CBF12A CRC64;
     MRKWYFILLA GVLTSVILAF VYDKTKANEE GSGDYLYVSP NGSDQNEGTK EKPFRTLAHA
     SEKAAAGTTV MIREGTYHET LDVKHSGTDG KPITFRNYEN ENVVISGESV ANAEYETPLI
     RIHDKHDIAI SGLTIQDLSV SSEEATAIGI YVSGSSSHIA IKDNHIRGIK TTADEGNAHG
     IAVYGTGSMK DIRIEDNTVE KLTLGASEAV VLNGNIDGFT VAGNVVRNNN NIGIDLIGYE
     GTADKNDYVR NGVVENNTVY QNSTYGNPAY GDEYSAGGIY VDGGHDIEIK NNTVYDNDIG
     IEATSEHKGK YANAIQITDN KVYNNAYTGI SIGGYDKKRG GTSNSLIARN IMYRNDTKGL
     YGGQLLLQYD TKNNTIEKNI LTAGDSRLFI GNDFTENEGN TVNHNVYHKE ADQDGIWMWK
     KKEYDSFSSY RKATKNDQQS IYADPMFRDE ASYDFSLDPD SPARPVIE