YWP1_CANAL
ID YWP1_CANAL Reviewed; 533 AA.
AC Q59Y31; A0A1D8PI92;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Yeast-form wall Protein 1;
DE AltName: Full=GPI-anchored protein 24;
DE AltName: Full=Thiol-extractable peptide 1;
DE AltName: Full=flocculation protein 1;
DE Flags: Precursor;
GN Name=YWP1; Synonyms=FLO1, PGA24, TEP1; OrderedLocusNames=CAALFM_C208590WA;
GN ORFNames=CaO19.11101, CaO19.3618;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP PROTEIN SEQUENCE OF 22-33, GLYCOSYLATION, DISRUPTION PHENOTYPE, FUNCTION,
RP INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15870471; DOI=10.1099/mic.0.27663-0;
RA Granger B.L., Flenniken M.L., Davis D.A., Mitchell A.P., Cutler J.E.;
RT "Yeast wall protein 1 of Candida albicans.";
RL Microbiology 151:1631-1644(2005).
RN [5]
RP INDUCTION.
RX PubMed=14555484; DOI=10.1128/ec.2.5.1018-1024.2003;
RA Chauhan N., Inglis D., Roman E., Pla J., Li D., Calera J.A., Calderone R.;
RT "Candida albicans response regulator gene SSK1 regulates a subset of genes
RT whose functions are associated with cell wall biosynthesis and adaptation
RT to oxidative stress.";
RL Eukaryot. Cell 2:1018-1024(2003).
RN [6]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [7]
RP INDUCTION.
RX PubMed=15218092; DOI=10.1091/mbc.e03-11-0782;
RA Doedt T., Krishnamurthy S., Bockmuhl D.P., Tebarth B., Stempel C.,
RA Russell C.L., Brown A.J., Ernst J.F.;
RT "APSES proteins regulate morphogenesis and metabolism in Candida
RT albicans.";
RL Mol. Biol. Cell 15:3167-3180(2004).
RN [8]
RP INDUCTION.
RX PubMed=16151249; DOI=10.1128/ec.4.9.1562-1573.2005;
RA Murillo L.A., Newport G., Lan C.Y., Habelitz S., Dungan J., Agabian N.M.;
RT "Genome-wide transcription profiling of the early phase of biofilm
RT formation by Candida albicans.";
RL Eukaryot. Cell 4:1562-1573(2005).
RN [9]
RP INDUCTION.
RX PubMed=15814841; DOI=10.1091/mbc.e05-01-0071;
RA Garcia-Sanchez S., Mavor A.L., Russell C.L., Argimon S., Dennison P.,
RA Enjalbert B., Brown A.J.;
RT "Global roles of Ssn6 in Tup1- and Nrg1-dependent gene regulation in the
RT fungal pathogen, Candida albicans.";
RL Mol. Biol. Cell 16:2913-2925(2005).
RN [10]
RP INDUCTION.
RX PubMed=16455273; DOI=10.1016/j.fgb.2005.12.002;
RA Castillo L., Martinez A.I., Garcera A., Garcia-Martinez J.,
RA Ruiz-Herrera J., Valentin E., Sentandreu R.;
RT "Genomic response programs of Candida albicans following protoplasting and
RT regeneration.";
RL Fungal Genet. Biol. 43:124-134(2006).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=17905924; DOI=10.1128/ec.00285-07;
RA Hiller E., Heine S., Brunner H., Rupp S.;
RT "Candida albicans Sun41p, a putative glycosidase, is involved in
RT morphogenesis, cell wall biogenesis, and biofilm formation.";
RL Eukaryot. Cell 6:2056-2065(2007).
RN [12]
RP INDUCTION.
RX PubMed=18558384; DOI=10.1016/s1672-0229(08)60019-4;
RA Altenburg S.D., Nielsen-Preiss S.M., Hyman L.E.;
RT "Increased filamentous growth of Candida albicans in simulated
RT microgravity.";
RL Genomics Proteomics Bioinformatics 6:42-50(2008).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=18712765; DOI=10.1002/pmic.200800110;
RA Castillo L., Calvo E., Martinez A.I., Ruiz-Herrera J., Valentin E.,
RA Lopez J.A., Sentandreu R.;
RT "A study of the Candida albicans cell wall proteome.";
RL Proteomics 8:3871-3881(2008).
RN [14]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=19555771; DOI=10.1016/j.fgb.2009.06.005;
RA Maddi A., Bowman S.M., Free S.J.;
RT "Trifluoromethanesulfonic acid-based proteomic analysis of cell wall and
RT secreted proteins of the ascomycetous fungi Neurospora crassa and Candida
RT albicans.";
RL Fungal Genet. Biol. 46:768-781(2009).
RN [15]
RP INDUCTION.
RX PubMed=20870877; DOI=10.1128/ec.00159-10;
RA Synnott J.M., Guida A., Mulhern-Haughey S., Higgins D.G., Butler G.;
RT "Regulation of the hypoxic response in Candida albicans.";
RL Eukaryot. Cell 9:1734-1746(2010).
RN [16]
RP SUBCELLULAR LOCATION, AND CLEAVAGE BY SAP9 AND SAP10.
RX PubMed=21097664; DOI=10.1128/ec.00210-10;
RA Schild L., Heyken A., de Groot P.W., Hiller E., Mock M., de Koster C.,
RA Horn U., Rupp S., Hube B.;
RT "Proteolytic cleavage of covalently linked cell wall proteins by Candida
RT albicans Sap9 and Sap10.";
RL Eukaryot. Cell 10:98-109(2011).
RN [17]
RP INDUCTION.
RX PubMed=21890817; DOI=10.1128/ec.05196-11;
RA Ganguly S., Bishop A.C., Xu W., Ghosh S., Nickerson K.W., Lanni F.,
RA Patton-Vogt J., Mitchell A.P.;
RT "Zap1 control of cell-cell signaling in Candida albicans biofilms.";
RL Eukaryot. Cell 10:1448-1454(2011).
RN [18]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=20864472; DOI=10.1099/mic.0.044206-0;
RA Sosinska G.J., de Koning L.J., de Groot P.W., Manders E.M., Dekker H.L.,
RA Hellingwerf K.J., de Koster C.G., Klis F.M.;
RT "Mass spectrometric quantification of the adaptations in the wall proteome
RT of Candida albicans in response to ambient pH.";
RL Microbiology 157:136-146(2011).
RN [19]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=21602216; DOI=10.1099/mic.0.049395-0;
RA Heilmann C.J., Sorgo A.G., Siliakus A.R., Dekker H.L., Brul S.,
RA de Koster C.G., de Koning L.J., Klis F.M.;
RT "Hyphal induction in the human fungal pathogen Candida albicans reveals a
RT characteristic wall protein profile.";
RL Microbiology 157:2297-2307(2011).
RN [20]
RP FUNCTION, INDUCTION, GPI-ANCHOR AT GLY-511, AND SUBCELLULAR LOCATION.
RX PubMed=22505336; DOI=10.1128/ec.00026-12;
RA Granger B.L.;
RT "Insight into the antiadhesive effect of yeast wall protein 1 of Candida
RT albicans.";
RL Eukaryot. Cell 11:795-805(2012).
RN [21]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=23243062; DOI=10.1128/ec.00278-12;
RA Heilmann C.J., Sorgo A.G., Mohammadi S., Sosinska G.J., de Koster C.G.,
RA Brul S., de Koning L.J., Klis F.M.;
RT "Surface stress induces a conserved cell wall stress response in the
RT pathogenic fungus Candida albicans.";
RL Eukaryot. Cell 12:254-264(2013).
RN [22]
RP INDUCTION, AND FUNCTION.
RX PubMed=23948468; DOI=10.1016/j.micpath.2013.07.003;
RA Tian J., Weng L.X., Zhang Y.Q., Wang L.H.;
RT "BDSF inhibits Candida albicans adherence to urinary catheters.";
RL Microb. Pathog. 64:33-38(2013).
RN [23]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=23136884; DOI=10.1111/mmi.12087;
RA Rohm M., Lindemann E., Hiller E., Ermert D., Lemuth K., Trkulja D.,
RA Sogukpinar O., Brunner H., Rupp S., Urban C.F., Sohn K.;
RT "A family of secreted pathogenesis-related proteins in Candida albicans.";
RL Mol. Microbiol. 87:132-151(2013).
CC -!- FUNCTION: Cell wall protein which plays an anti-adhesive role and
CC promotes dispersal of yeast forms, which allows the organism to seek
CC new sites for colonization. {ECO:0000269|PubMed:15870471,
CC ECO:0000269|PubMed:22505336, ECO:0000269|PubMed:23948468}.
CC -!- SUBCELLULAR LOCATION: Secreted. Secreted, cell wall. Membrane
CC {ECO:0000305}; Lipid-anchor, GPI-anchor {ECO:0000305}. Note=In yeast
CC cultures, is linked covalently to glucans of the wall matrix, but, as
CC cultures approach stationary phase, accumulates in the medium and is
CC extractable from cells with disulfide-reducing agents. Removed from the
CC cell wall by proteolytic cleavage by SAP9 and SAP10.
CC -!- INDUCTION: Expression is greatest during yeast exponential-phase
CC growth, but down-regulated in stationary phase and upon filamentation.
CC Expression is also increased during growth in hypoxic conditions.
CC Expression decreases in biofilm cultures and becomes undetectable
CC through late stage biofilm formation. Up-regulated when the
CC extracellular phosphate concentration is low or in presence of Cis-2-
CC dodecenoic acid (BDSF). Repressed during cell wall regeneration.
CC Expression is positively regulated by EFG1 and EFH1, and negatively
CC regulated by SSN6 and SSK1. {ECO:0000269|PubMed:14555484,
CC ECO:0000269|PubMed:15218092, ECO:0000269|PubMed:15814841,
CC ECO:0000269|PubMed:15870471, ECO:0000269|PubMed:16151249,
CC ECO:0000269|PubMed:16455273, ECO:0000269|PubMed:18558384,
CC ECO:0000269|PubMed:19555771, ECO:0000269|PubMed:20864472,
CC ECO:0000269|PubMed:20870877, ECO:0000269|PubMed:21602216,
CC ECO:0000269|PubMed:21890817, ECO:0000269|PubMed:22505336,
CC ECO:0000269|PubMed:23948468}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC {ECO:0000269|PubMed:21097664}.
CC -!- PTM: Cleaved by SAP9 and SAP10, which leads to its release from the
CC cell wall.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:15870471}.
CC -!- DISRUPTION PHENOTYPE: Leads to increased adhesion and biofilm
CC formation. {ECO:0000269|PubMed:15870471}.
CC -!- SIMILARITY: Belongs to the flocculin family. {ECO:0000305}.
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DR EMBL; CP017624; AOW27847.1; -; Genomic_DNA.
DR RefSeq; XP_714462.1; XM_709369.2.
DR AlphaFoldDB; Q59Y31; -.
DR STRING; 237561.Q59Y31; -.
DR GeneID; 3643915; -.
DR KEGG; cal:CAALFM_C208590WA; -.
DR CGD; CAL0000183265; YWP1.
DR VEuPathDB; FungiDB:C2_08590W_A; -.
DR eggNOG; ENOG502S4SV; Eukaryota.
DR HOGENOM; CLU_029456_0_0_1; -.
DR InParanoid; Q59Y31; -.
DR OrthoDB; 1633983at2759; -.
DR PRO; PR:Q59Y31; -.
DR Proteomes; UP000000559; Chromosome 2.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009986; C:cell surface; IDA:CGD.
DR GO; GO:0005576; C:extracellular region; IDA:CGD.
DR GO; GO:1903561; C:extracellular vesicle; IDA:CGD.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:CGD.
DR GO; GO:0030446; C:hyphal cell wall; IDA:CGD.
DR GO; GO:0030445; C:yeast-form cell wall; IDA:CGD.
DR GO; GO:0044406; P:adhesion of symbiont to host; IDA:CGD.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0044010; P:single-species biofilm formation; IMP:CGD.
DR InterPro; IPR025928; Flocculin_t3_rpt.
DR Pfam; PF13928; Flocculin_t3; 3.
PE 1: Evidence at protein level;
KW Cell adhesion; Cell wall; Direct protein sequencing; Glycoprotein;
KW GPI-anchor; Lipoprotein; Membrane; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:15870471"
FT CHAIN 22..511
FT /note="Yeast-form wall Protein 1"
FT /id="PRO_0000424775"
FT PROPEP 512..533
FT /note="Removed in mature form"
FT /id="PRO_0000424776"
FT REGION 161..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 418..451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 511
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000269|PubMed:22505336"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 533 AA; 54278 MW; 257F9C08A1D0A206 CRC64;
MKVSTIFAAA SALFAATTTL AQDVACLVDN QQVAVVDLDT GVCPFTIPAS LAAFFTFVSL
EEYNVQFYYT IVNNVRYNTD IRNAGKVINV PARNLYGAGA VPFFQVHLEK QLEANSTAAI
RRRLMGETPI VKRDQIDDFI ASIENTEGTA LEGSTLEVVD YVPGSSSASP SGSASPSGSE
SGSGSDSATI RSTTVVSSSS CESSGDSAAT ATGANGESTV TEQNTVVVTI TSCHNDACHA
TTVPATASIG VTTVHGTETI FTTYCPLSSY ETVESTKVIT ITSCSENKCQ ETTVEATPST
ATTVSEGVVT EYVTYCPVSS VETVASTKVI TVVACDEHKC HETTAVATPT EVTTVVEGST
THYVTYKPTG SGPTQGETYA TNAITSEGTV YVPKTTAVTT HGSTFETVAY ITVTKATPTK
GGEQHQPGSP AGAATSAPGA PAPGASGAHA STANKVTVEA QATPGTLTPE NTVAGGVNGE
QVAVSAKTTI SQTTVAKASG SGKAAISTFE GAAAASAGAS VLALALIPLA YFI
