YWPE_BACSU
ID YWPE_BACSU Reviewed; 102 AA.
AC P94587; Q795A8;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Putative sortase YwpE {ECO:0000305};
DE EC=3.4.22.- {ECO:0000250|UniProtKB:P54603};
GN Name=ywpE; OrderedLocusNames=BSU36340;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA Kunst F., Danchin A., Glaser P.;
RT "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT degrees).";
RL Microbiology 143:3313-3328(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION, AND INDUCTION.
RX PubMed=21906378; DOI=10.1186/2191-0855-1-22;
RA Nguyen H.D., Phan T.T., Schumann W.;
RT "Analysis and application of Bacillus subtilis sortases to anchor
RT recombinant proteins on the cell wall.";
RL AMB Express 1:22-22(2011).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=21800427; DOI=10.1002/pmic.201100174;
RA Fasehee H., Westers H., Bolhuis A., Antelmann H., Hecker M., Quax W.J.,
RA Mirlohi A.F., van Dijl J.M., Ahmadian G.;
RT "Functional analysis of the sortase YhcS in Bacillus subtilis.";
RL Proteomics 11:3905-3913(2011).
CC -!- FUNCTION: Seems not to play a major role if any as a sortase.
CC {ECO:0000305|PubMed:21906378}.
CC -!- INDUCTION: Preferentially expressed in the late stationary phase.
CC {ECO:0000269|PubMed:21906378}.
CC -!- DISRUPTION PHENOTYPE: Mutation does not affect extracellular levels of
CC the sortase substrate YfkN. {ECO:0000269|PubMed:21800427}.
CC -!- SIMILARITY: Belongs to the bacterial sortase family. {ECO:0000305}.
CC -!- CAUTION: Compared to other sortases, it is shorter and lacks the N-
CC terminal membrane anchor. It may lack activity. {ECO:0000305}.
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DR EMBL; Z83337; CAB05946.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15651.1; -; Genomic_DNA.
DR PIR; G70065; G70065.
DR RefSeq; NP_391515.1; NC_000964.3.
DR RefSeq; WP_003227792.1; NC_000964.3.
DR AlphaFoldDB; P94587; -.
DR SMR; P94587; -.
DR STRING; 224308.BSU36340; -.
DR MEROPS; C60.A01; -.
DR PaxDb; P94587; -.
DR PRIDE; P94587; -.
DR EnsemblBacteria; CAB15651; CAB15651; BSU_36340.
DR GeneID; 936904; -.
DR KEGG; bsu:BSU36340; -.
DR PATRIC; fig|224308.179.peg.3933; -.
DR eggNOG; COG3764; Bacteria.
DR OMA; TCDKAVK; -.
DR PhylomeDB; P94587; -.
DR BioCyc; BSUB:BSU36340-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd06165; Sortase_A; 1.
DR Gene3D; 2.40.260.10; -; 1.
DR InterPro; IPR005754; Sortase.
DR InterPro; IPR042007; Sortase_A.
DR InterPro; IPR023365; Sortase_dom-sf.
DR Pfam; PF04203; Sortase; 1.
DR SUPFAM; SSF63817; SSF63817; 1.
DR TIGRFAMs; TIGR01076; sortase_fam; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Protease; Reference proteome; Thiol protease.
FT CHAIN 1..102
FT /note="Putative sortase YwpE"
FT /id="PRO_0000390896"
FT ACT_SITE 17
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q2FV99"
FT ACT_SITE 78
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q2FV99"
FT SITE 87
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250|UniProtKB:Q2FV99"
SQ SEQUENCE 102 AA; 11468 MW; D5820A990E577FF8 CRC64;
MRRDQKMGEG NYPLAGHHLK QKNLLFGPLE NIKTGAQIVI TDFKKDYIYS VTSKDIISEM
DADVVEETNK KEITLITCDK AVKTEGRLVV KGELVDSFGH TN
