ID   YWQK_BACSU              Reviewed;         154 AA.
AC   P96723; Q795B7;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Immunity protein YwqK;
GN   Name=ywqK; OrderedLocusNames=BSU36180;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA   Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA   Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA   Kunst F., Danchin A., Glaser P.;
RT   "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT   degrees).";
RL   Microbiology 143:3313-3328(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   PROBABLE FUNCTION AS AN IMMUNITY PROTEIN, AND PROBABLE SUBUNIT.
RC   STRAIN=168;
RX   PubMed=22200572; DOI=10.1016/j.febslet.2011.12.020;
RA   Holberger L.E., Garza-Sanchez F., Lamoureux J., Low D.A., Hayes C.S.;
RT   "A novel family of toxin/antitoxin proteins in Bacillus species.";
RL   FEBS Lett. 586:132-136(2012).
RN   [4]
RP   FUNCTION AS AN IMMUNITY PROTEIN, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=34280190; DOI=10.1371/journal.pgen.1009682;
RA   Kobayashi K.;
RT   "Diverse LXG toxin and antitoxin systems specifically mediate intraspecies
RT   competition in Bacillus subtilis biofilms.";
RL   PLoS Genet. 17:e1009682-e1009682(2021).
CC   -!- FUNCTION: Immunity component of one of 6 LXG toxin-immunity modules in
CC       this strain. They promote kin selection, mediate competition in
CC       biofilms, and drive spatial segregation of different strains,
CC       indicating that LXG toxins may help avoid warfare between strains in
CC       biofilms. Mediates intercellular competition during biofilm formation;
CC       disruption of the operon disadvantages the bacteria, but overexpression
CC       of the cognate immunity protein restores growth in competition with
CC       wild-type. In situ neutralizes the toxic effect of cognate toxin YqcG
CC       (PubMed:34280190). Probably neutralizes the ability to inhibit growth
CC       of cognate toxin YwqJ. Probably does not have immunity protein activity
CC       on other LXG toxins (Probable). {ECO:0000269|PubMed:34280190,
CC       ECO:0000305|PubMed:22200572}.
CC   -!- SUBUNIT: Probably interacts with cognate toxin YwqJ but not with other
CC       non-cognate LXG toxins. The interaction inhibits the toxic activity of
CC       YwqJ. {ECO:0000305|PubMed:22200572}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Expressed on rich and minimal solid media likely in early
CC       stationary phase; dependent on DegSU. Not expressed in liquid LB, but
CC       only under conditions that promote biofilm formation.
CC       {ECO:0000269|PubMed:34280190}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the ywqH-ywqI-ywqJ-ywqK-nfi operon
CC       has no visible growth phenotype, however it is out-competed by wild-
CC       type cells. {ECO:0000269|PubMed:34280190}.
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DR   EMBL; Z92952; CAB07449.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15635.1; -; Genomic_DNA.
DR   PIR; F70067; F70067.
DR   RefSeq; NP_391499.1; NC_000964.3.
DR   RefSeq; WP_003243602.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P96723; -.
DR   SMR; P96723; -.
DR   STRING; 224308.BSU36180; -.
DR   PaxDb; P96723; -.
DR   PRIDE; P96723; -.
DR   EnsemblBacteria; CAB15635; CAB15635; BSU_36180.
DR   GeneID; 936888; -.
DR   KEGG; bsu:BSU36180; -.
DR   PATRIC; fig|224308.179.peg.3915; -.
DR   eggNOG; COG2849; Bacteria.
DR   InParanoid; P96723; -.
DR   OMA; WFTSYSD; -.
DR   BioCyc; BSUB:BSU36180-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   InterPro; IPR011652; MORN_2.
DR   Pfam; PF07661; MORN_2; 2.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome; Toxin.
FT   CHAIN           1..154
FT                   /note="Immunity protein YwqK"
FT                   /id="PRO_0000375915"
SQ   SEQUENCE   154 AA;  18217 MW;  D52057841E1804B5 CRC64;
     MENEYDMKSI KEKGVDFEDL WFSSVSDEIL DNPEDENGQP FTGLAYELYP NGQIIYFTKY
     KNGLAHGLTC EFYENGNKKS EKEYRYGQLH GISIIWFENG RKKSEQQYEH SILISEKNWD
     EEGNLLNKYE IDTDSPHFEI LESRRETHIN LGRE