ID   YXDJ_BACSU              Reviewed;         229 AA.
AC   P42421;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Transcriptional regulatory protein YxdJ;
GN   Name=yxdJ; OrderedLocusNames=BSU39660; ORFNames=B65D;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / BGSC1A1;
RX   PubMed=7952181; DOI=10.1099/13500872-140-9-2289;
RA   Yoshida K., Sano H., Miwa Y., Ogasawara N., Fujita Y.;
RT   "Cloning and nucleotide sequencing of a 15 kb region of the Bacillus
RT   subtilis genome containing the iol operon.";
RL   Microbiology 140:2289-2298(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION.
RX   PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA   Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA   Fujita Y.;
RT   "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT   regulatory systems.";
RL   J. Bacteriol. 183:7365-7370(2001).
RN   [4]
RP   FUNCTION.
RC   STRAIN=168;
RX   PubMed=15289557; DOI=10.1099/mic.0.27155-0;
RA   Joseph P., Guiseppi A., Sorokin A., Denizot F.;
RT   "Characterization of the Bacillus subtilis YxdJ response regulator as the
RT   inducer of expression for the cognate ABC transporter YxdLM.";
RL   Microbiology 150:2609-2617(2004).
RN   [5]
RP   FUNCTION.
RC   STRAIN=168;
RX   PubMed=15870467; DOI=10.1099/mic.0.27761-0;
RA   Pietiaeinen M., Gardemeister M., Mecklin M., Leskelae S., Sarvas M.,
RA   Kontinen V.P.;
RT   "Cationic antimicrobial peptides elicit a complex stress response in
RT   Bacillus subtilis that involves ECF-type sigma factors and two-component
RT   signal transduction systems.";
RL   Microbiology 151:1577-1592(2005).
CC   -!- FUNCTION: Probable member of the two-component regulatory system
CC       YxdK/YxdJ. Positively regulates the expression of the yxdLMyxeA operon
CC       by direct interaction with its promoter region. Could also indirectly
CC       regulate the expression of the dlt operon.
CC       {ECO:0000269|PubMed:11717295, ECO:0000269|PubMed:15289557,
CC       ECO:0000269|PubMed:15870467}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by YxdK. {ECO:0000305}.
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DR   EMBL; D14399; BAA03300.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB16002.1; -; Genomic_DNA.
DR   PIR; G70073; G70073.
DR   RefSeq; NP_391845.1; NC_000964.3.
DR   RefSeq; WP_003243527.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P42421; -.
DR   SMR; P42421; -.
DR   IntAct; P42421; 1.
DR   STRING; 224308.BSU39660; -.
DR   PaxDb; P42421; -.
DR   PRIDE; P42421; -.
DR   EnsemblBacteria; CAB16002; CAB16002; BSU_39660.
DR   GeneID; 937599; -.
DR   KEGG; bsu:BSU39660; -.
DR   PATRIC; fig|224308.179.peg.4291; -.
DR   eggNOG; COG0745; Bacteria.
DR   InParanoid; P42421; -.
DR   OMA; VWEYGFG; -.
DR   PhylomeDB; P42421; -.
DR   BioCyc; BSUB:BSU39660-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..229
FT                   /note="Transcriptional regulatory protein YxdJ"
FT                   /id="PRO_0000081397"
FT   DOMAIN          3..116
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        129..227
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         52
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   229 AA;  26600 MW;  A540FCB081A8587C CRC64;
     MNKIMIVEDS EDIRGLLQNY LEKYGYQTVV AADFTAVLDV FLREKPDVVL LDINLPAYDG
     YYWCRQIRQH STSPIIFISA RSGEMDQVMA IENGGDDYIE KPFSYDIVLA KIKSQIRRAY
     GEYAAKQGEK VVEYAGVQLF VERFELRFQD EKSELSKKES KLLEVLLERG EKVTSRDRLM
     EKTWDTDIFI DDNTLNVYIT RLRKKLRELN APVSIEAVRG EGYQLRAQS