YXIM_BACSU
ID YXIM_BACSU Reviewed; 382 AA.
AC P42304;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Uncharacterized esterase YxiM;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN Name=yxiM; OrderedLocusNames=BSU39120; ORFNames=SS8D;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RX PubMed=8969509; DOI=10.1099/13500872-142-11-3113;
RA Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y.,
RA Fujita Y.;
RT "Sequencing of a 65 kb region of the Bacillus subtilis genome containing
RT the lic and cel loci, and creation of a 177 kb contig covering the gnt-
RT sacXY region.";
RL Microbiology 142:3113-3123(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 83.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 17-382.
RG Northeast structural genomics consortium (NESG);
RT "X-ray structure of the hypothetical protein YXIM_BACSU from Bacillus
RT subtilis.";
RL Submitted (DEC-2006) to the PDB data bank.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; D83026; BAA11692.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15948.2; -; Genomic_DNA.
DR PIR; A70078; A70078.
DR RefSeq; NP_391791.2; NC_000964.3.
DR RefSeq; WP_003242617.1; NZ_JNCM01000034.1.
DR PDB; 2O14; X-ray; 2.10 A; A=17-382.
DR PDBsum; 2O14; -.
DR AlphaFoldDB; P42304; -.
DR SMR; P42304; -.
DR STRING; 224308.BSU39120; -.
DR PaxDb; P42304; -.
DR PRIDE; P42304; -.
DR EnsemblBacteria; CAB15948; CAB15948; BSU_39120.
DR GeneID; 937485; -.
DR KEGG; bsu:BSU39120; -.
DR PATRIC; fig|224308.179.peg.4236; -.
DR eggNOG; COG2755; Bacteria.
DR eggNOG; COG3401; Bacteria.
DR InParanoid; P42304; -.
DR OMA; FQVRNMA; -.
DR PhylomeDB; P42304; -.
DR BioCyc; BSUB:BSU39120-MON; -.
DR EvolutionaryTrace; P42304; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IBA:GO_Central.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IBA:GO_Central.
DR CDD; cd01821; Rhamnogalacturan_acetylesterase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR037459; RhgT-like.
DR InterPro; IPR013830; SGNH_hydro.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR PANTHER; PTHR43695; PTHR43695; 1.
DR Pfam; PF13472; Lipase_GDSL_2; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..382
FT /note="Uncharacterized esterase YxiM"
FT /id="PRO_0000013744"
FT ACT_SITE 186
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 354
FT /evidence="ECO:0000250"
FT ACT_SITE 357
FT /evidence="ECO:0000250"
FT CONFLICT 83
FT /note="Q -> E (in Ref. 1; BAA11692)"
FT /evidence="ECO:0000305"
FT STRAND 30..38
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 44..47
FT /evidence="ECO:0007829|PDB:2O14"
FT TURN 55..57
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 58..62
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 64..66
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 67..71
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 76..79
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 80..86
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 95..98
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 101..112
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 114..120
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 123..131
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 135..142
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 144..155
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 161..173
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 179..184
FT /evidence="ECO:0007829|PDB:2O14"
FT TURN 186..188
FT /evidence="ECO:0007829|PDB:2O14"
FT TURN 194..196
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 202..205
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 206..208
FT /evidence="ECO:0007829|PDB:2O14"
FT TURN 212..214
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 216..219
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 227..232
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 235..240
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 248..252
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 256..258
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 260..262
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 266..281
FT /evidence="ECO:0007829|PDB:2O14"
FT TURN 282..284
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 286..290
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 313..322
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 326..328
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 330..341
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 343..347
FT /evidence="ECO:0007829|PDB:2O14"
FT STRAND 355..359
FT /evidence="ECO:0007829|PDB:2O14"
FT HELIX 360..376
FT /evidence="ECO:0007829|PDB:2O14"
SQ SEQUENCE 382 AA; 41836 MW; B46ADC4054B3C97D CRC64;
MKKWMAAVFV MMLMLCFGGI ENVKAAEPKV YQFDFGSGSM EPGYIGVRAS DRYDRSKGYG
FQTPENMRDV AASGAGVKSD AVQFLAYGTK SNNTFNVDLP NGLYEVKVTL GNTARASVAA
EGVFQVINMT GDGAEDTFQI PVTDGQLNLL VTEGKAGTAF TLSALKIKKL SDQPVTNRTI
YVGGDSTVCN YYPLNSSKQA GWGQMLPHYI DKHTFQVRNM ASGGQIARGF RNDGQLEAIL
KYIKPGDYFM LQLGINDTNP KHKESEAEFK EVMRDMIRQV KAKGADVILS TPQGRATDFT
SEGIHSSVNR WYRASILALA EEEKTYLIDL NVLSSAYFTS IGPERTLGLY MDGDTLHPNR
AGADALARLA VQELKRQGIA GF
