YXXD_BACSU
ID YXXD_BACSU Reviewed; 147 AA.
AC P40737;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Immunity protein YxxD;
DE AltName: Full=ORF1;
GN Name=yxxD; OrderedLocusNames=BSU39290; ORFNames=N17A;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RX PubMed=7704263; DOI=10.1099/13500872-141-2-337;
RA Yoshida K., Sano H., Seki S., Oda M., Fujimura M., Fujita Y.;
RT "Cloning and sequencing of a 29 kb region of the Bacillus subtilis genome
RT containing the hut and wapA loci.";
RL Microbiology 141:337-343(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8628237; DOI=10.1007/bf02172988;
RA Beloin C., Hirschbein L., Le Hegarat F.;
RT "Suppression of the Bgl+ phenotype of a delta hns strain of Escherichia
RT coli by a Bacillus subtilis antiterminator binding site.";
RL Mol. Gen. Genet. 250:761-766(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-147.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=7883710; DOI=10.1128/jb.177.6.1527-1535.1995;
RA Le Coq D., Lindner C., Krueger S., Steinmetz M., Stuelke J.;
RT "New beta-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product
RT has both transport and regulatory functions similar to those of BglF, its
RT Escherichia coli homolog.";
RL J. Bacteriol. 177:1527-1535(1995).
RN [5]
RP FUNCTION AS AN IMMUNITY PROTEIN, AND EXPRESSION IN E.COLI.
RC STRAIN=168;
RX PubMed=22200572; DOI=10.1016/j.febslet.2011.12.020;
RA Holberger L.E., Garza-Sanchez F., Lamoureux J., Low D.A., Hayes C.S.;
RT "A novel family of toxin/antitoxin proteins in Bacillus species.";
RL FEBS Lett. 586:132-136(2012).
RN [6]
RP FUNCTION AS AN IMMUNITY PROTEIN, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=34280190; DOI=10.1371/journal.pgen.1009682;
RA Kobayashi K.;
RT "Diverse LXG toxin and antitoxin systems specifically mediate intraspecies
RT competition in Bacillus subtilis biofilms.";
RL PLoS Genet. 17:e1009682-e1009682(2021).
CC -!- FUNCTION: Immunity component of one of 6 LXG toxin-immunity modules in
CC this strain. They promote kin selection, mediate competition in
CC biofilms, and drive spatial segregation of different strains,
CC indicating that LXG toxins may help avoid warfare between strains in
CC biofilms. Mediates intercellular competition during biofilm formation;
CC disruption of the operon disadvantages the bacteria, but overexpression
CC of the cognate immunity protein restores growth in competition with
CC wild-type. In situ neutralizes the toxic effect of cognate toxin YxiD
CC (PubMed:34280190). Neutralizes the toxic activity of cognate toxin YxiD
CC upon expression in E.coli. Does not have immunity protein activity on
CC other LXG toxins (PubMed:22200572). {ECO:0000269|PubMed:22200572,
CC ECO:0000269|PubMed:34280190}.
CC -!- SUBUNIT: Probably interacts with cognate toxin YxiD but not with other
CC non-cognate toxins. The interaction inhibits the toxic activity of YxiD
CC (Probable). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expressed on rich and minimal solid media likely in early
CC stationary phase; dependent on DegSU. Not expressed in liquid LB, but
CC only under conditions that promote biofilm formation.
CC {ECO:0000269|PubMed:34280190}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the yxiB-yxiC-yxiD-yxxD-yxxE operon
CC has no visible growth phenotype, however it is out-competed by wild-
CC type cells. {ECO:0000269|PubMed:34280190}.
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DR EMBL; D29985; BAA06254.1; -; Genomic_DNA.
DR EMBL; D31856; BAA06650.1; -; Genomic_DNA.
DR EMBL; X85408; CAA59695.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15965.1; -; Genomic_DNA.
DR EMBL; Z34526; CAA84284.1; -; Genomic_DNA.
DR PIR; S65579; S65579.
DR RefSeq; NP_391808.1; NC_000964.3.
DR RefSeq; WP_003244256.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P40737; -.
DR SMR; P40737; -.
DR STRING; 224308.BSU39290; -.
DR PaxDb; P40737; -.
DR PRIDE; P40737; -.
DR EnsemblBacteria; CAB15965; CAB15965; BSU_39290.
DR GeneID; 937530; -.
DR KEGG; bsu:BSU39290; -.
DR PATRIC; fig|224308.179.peg.4253; -.
DR eggNOG; ENOG5031IMN; Bacteria.
DR OMA; YNINRIM; -.
DR BioCyc; BSUB:BSU39290-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR Gene3D; 3.40.1580.10; -; 1.
DR InterPro; IPR018958; Knr4/Smi1-like_dom.
DR InterPro; IPR037883; Knr4/Smi1-like_sf.
DR SMART; SM00860; SMI1_KNR4; 1.
DR SUPFAM; SSF160631; SSF160631; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome.
FT CHAIN 1..147
FT /note="Immunity protein YxxD"
FT /id="PRO_0000050043"
SQ SEQUENCE 147 AA; 17665 MW; D7A71B9E61B676DE CRC64;
MSYNFIKSNK ENDFYPVNQS EIEEVEKNLN LKLPSELVNF YLEVGYGFIK GSEFNTNRIL
DPYSVRDFRL RINDFEFYPD IEIYDEFEND KLIFFEGSES ALMSIELNDN NKNPIYYYDI
QIATSLTEFL RKIEENDQYY LDLLDDE
