YYDG_BACSU
ID YYDG_BACSU Reviewed; 319 AA.
AC Q45595; Q794X0;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Putative peptide biosynthesis protein YydG;
GN Name=yydG; OrderedLocusNames=BSU40170;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9205843; DOI=10.1093/dnares/4.2.155;
RA Kasahara Y., Nakai S., Ogasawara N.;
RT "Sequence analysis of the 36-kb region between gntZ and trnY genes of
RT Bacillus subtilis genome.";
RL DNA Res. 4:155-159(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SUGGESTION OF FUNCTION, AND OPERON STRUCTURE.
RC STRAIN=168;
RX PubMed=17921301; DOI=10.1128/jb.01181-07;
RA Butcher B.G., Lin Y.-P., Helmann J.D.;
RT "The yydFGHIJ operon of Bacillus subtilis encodes a peptide that induces
RT the LiaRS two-component system.";
RL J. Bacteriol. 189:8616-8625(2007).
CC -!- FUNCTION: Required for production of the modified peptide YydF
CC (Probable). May activate a metalloenzyme (Potential). {ECO:0000305}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000305};
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DR EMBL; D78193; BAA11275.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB16054.1; -; Genomic_DNA.
DR PIR; E70091; E70091.
DR RefSeq; NP_391897.1; NC_000964.3.
DR RefSeq; WP_003242609.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; Q45595; -.
DR SMR; Q45595; -.
DR STRING; 224308.BSU40170; -.
DR PaxDb; Q45595; -.
DR PRIDE; Q45595; -.
DR EnsemblBacteria; CAB16054; CAB16054; BSU_40170.
DR GeneID; 937720; -.
DR KEGG; bsu:BSU40170; -.
DR PATRIC; fig|224308.179.peg.4345; -.
DR eggNOG; COG0535; Bacteria.
DR InParanoid; Q45595; -.
DR OMA; EISACCG; -.
DR BioCyc; BSUB:BSU40170-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR023904; Pep_rSAM_mat_YydG.
DR InterPro; IPR007197; rSAM.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR TIGRFAMs; TIGR04078; rSAM_yydG; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW S-adenosyl-L-methionine.
FT CHAIN 1..319
FT /note="Putative peptide biosynthesis protein YydG"
FT /id="PRO_0000370198"
FT DOMAIN 1..214
FT /note="Radical SAM core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT BINDING 14
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000255"
FT BINDING 18
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000255"
FT BINDING 21
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000255"
SQ SEQUENCE 319 AA; 37212 MW; 5A72B798A389040B CRC64;
MYNKTVSINL DSRCNASCDH CCFSSSPTST TRMEKEYIRE LVTEFAKNKT IQVISFTGGE
VFLDYKFLKE LMEIIKPYEK QITLISNGFW GLSKKKVQEY FHDMNSLNVI ALTISYDEYH
APFVKSSSIK NILEHSRKYP DIDISLNMAV TKDKMSNHIL EELGDSILGV KITKFPMISV
GAAKTRIKQE NIHKFYSLED EDSLHCPGYD IVYHHDGEIY PCCSPAIFET KITLREEYNQ
SFERTVEKLN SNLLLFILRK EGFKWFLNIL KENNKIEEFD IPYEFSSICG VCGSLFNSAE
KINYFYPYME KYYNENFKV
