Z2753_HALVD
ID Z2753_HALVD Reviewed; 59 AA.
AC D4GWB3; A0A384LLU8; L9V568;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Zinc finger protein HVO_2753 {ECO:0000303|PubMed:32905660};
DE AltName: Full=Small CPxCG-related zinc finger protein {ECO:0000312|EMBL:ADE04009.1};
DE AltName: Full=Zinc finger u-protein HVO_2753 {ECO:0000303|PubMed:32905660};
DE AltName: Full=Zn-ribbon RNA-binding protein {ECO:0000312|EMBL:ELY32345.1};
GN OrderedLocusNames=HVO_2753 {ECO:0000312|EMBL:ADE04009.1};
GN ORFNames=C498_09054 {ECO:0000312|EMBL:ELY32345.1};
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800 {ECO:0000312|EMBL:ADE04009.1};
RN [1] {ECO:0000312|EMBL:ADE04009.1, ECO:0000312|Proteomes:UP000008243}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2 {ECO:0000312|Proteomes:UP000008243};
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
RN [2] {ECO:0000312|EMBL:ELY32345.1, ECO:0000312|Proteomes:UP000011532}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2 {ECO:0000312|Proteomes:UP000011532};
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
RN [3] {ECO:0007744|PDB:6YDH}
RP STRUCTURE BY NMR OF 2-59, FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE,
RP MUTAGENESIS OF CYS-12; CYS-29; GLN-34; CYS-39; TYR-49 AND CYS-51, AND
RP PHYLOGENETIC ANALYSIS.
RC STRAIN=DS2 / DS70 {ECO:0000303|PubMed:32905660};
RX PubMed=32905660; DOI=10.1111/febs.15559;
RA Zahn S., Kubatova N., Pyper D.J., Cassidy L., Saxena K., Tholey A.,
RA Schwalbe H., Soppa J.;
RT "Biological functions, genetic and biochemical characterization, and NMR
RT structure determination of the small zinc finger protein HVO_2753 from
RT Haloferax volcanii.";
RL FEBS J. 288:2042-2062(2021).
CC -!- FUNCTION: Zinc-binding protein. Binds 1 zinc ion. Required for
CC swarming. Involved in biofilm formation. {ECO:0000269|PubMed:32905660}.
CC -!- SUBUNIT: Monomer in solution. {ECO:0000269|PubMed:32905660}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene grow as the wild-type in
CC complex medium and in synthetic medium with glucose as sole carbon and
CC energy source, but the mutant cells are not capable of swarming and
CC have severe defects in forming biofilms. {ECO:0000269|PubMed:32905660}.
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DR EMBL; CP001956; ADE04009.1; -; Genomic_DNA.
DR EMBL; AOHU01000047; ELY32345.1; -; Genomic_DNA.
DR RefSeq; WP_004042997.1; NZ_AOHU01000047.1.
DR PDB; 6YDH; NMR; -; A=2-59.
DR PDBsum; 6YDH; -.
DR SMR; D4GWB3; -.
DR STRING; 309800.C498_09054; -.
DR EnsemblBacteria; ADE04009; ADE04009; HVO_2753.
DR EnsemblBacteria; ELY32345; ELY32345; C498_09054.
DR GeneID; 8925692; -.
DR KEGG; hvo:HVO_2753; -.
DR PATRIC; fig|309800.29.peg.1774; -.
DR eggNOG; arCOG01989; Archaea.
DR HOGENOM; CLU_196471_0_0_2; -.
DR OMA; KCGFEGP; -.
DR OrthoDB; 123853at2157; -.
DR Proteomes; UP000008243; Chromosome.
DR Proteomes; UP000011532; Unassembled WGS sequence.
DR GO; GO:0008270; F:zinc ion binding; IDA:UniProtKB.
DR GO; GO:0097590; P:archaeal-type flagellum-dependent cell motility; IMP:UniProtKB.
DR GO; GO:0090606; P:single-species surface biofilm formation; IMP:UniProtKB.
DR InterPro; IPR044720; HVO_2753-like.
DR InterPro; IPR011668; HVO_2753-like_ZBP.
DR PANTHER; PTHR40733; PTHR40733; 1.
DR Pfam; PF07754; HVO_2753_ZBP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Zinc.
FT CHAIN 1..59
FT /note="Zinc finger protein HVO_2753"
FT /id="PRO_0000454761"
FT MOTIF 29..33
FT /note="Zinc-binding pocket C(P)XCG"
FT /evidence="ECO:0000305|PubMed:32905660"
FT MOTIF 51..55
FT /note="Zinc-binding pocket C(P)XCG"
FT /evidence="ECO:0000305|PubMed:32905660"
FT MUTAGEN 12
FT /note="C->A: Not able to complement the deletion mutant of
FT this gene. Loss of swarming. Severe loss of structure and
FT stability."
FT /evidence="ECO:0000269|PubMed:32905660"
FT MUTAGEN 29
FT /note="C->A: Not able to complement the deletion mutant of
FT this gene. Loss of swarming. Severe loss of structure and
FT stability."
FT /evidence="ECO:0000269|PubMed:32905660"
FT MUTAGEN 34
FT /note="Q->A: Not able to complement the deletion mutant of
FT this gene. Loss of swarming. Severe loss of structure and
FT stability."
FT /evidence="ECO:0000269|PubMed:32905660"
FT MUTAGEN 39
FT /note="C->A: Not able to complement the deletion mutant of
FT this gene. Loss of swarming. Severe loss of structure and
FT stability."
FT /evidence="ECO:0000269|PubMed:32905660"
FT MUTAGEN 49
FT /note="Y->F: Not able to complement the deletion mutant of
FT this gene. Loss of swarming. Severe loss of structure and
FT stability."
FT /evidence="ECO:0000269|PubMed:32905660"
FT MUTAGEN 51
FT /note="C->A: Not able to complement the deletion mutant of
FT this gene. Loss of swarming. Severe loss of structure and
FT stability."
FT /evidence="ECO:0000269|PubMed:32905660"
FT STRAND 13..15
FT /evidence="ECO:0007829|PDB:6YDH"
FT STRAND 22..28
FT /evidence="ECO:0007829|PDB:6YDH"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:6YDH"
FT STRAND 35..38
FT /evidence="ECO:0007829|PDB:6YDH"
FT HELIX 42..45
FT /evidence="ECO:0007829|PDB:6YDH"
FT STRAND 48..51
FT /evidence="ECO:0007829|PDB:6YDH"
FT TURN 52..55
FT /evidence="ECO:0007829|PDB:6YDH"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:6YDH"
SQ SEQUENCE 59 AA; 6432 MW; 8B128A35595E8E4B CRC64;
MSESEQRHAH QCVSCGINIA GMSAATFKCP DCGQEISRCS KCRKQSNLYE CPDCGFMGP
