Z324B_HUMAN
ID Z324B_HUMAN Reviewed; 544 AA.
AC Q6AW86; B2RTZ6; Q6ZMX8; Q6ZS42;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Zinc finger protein 324B;
GN Name=ZNF324B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Endometrial adenocarcinoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-214, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25218447; DOI=10.1038/nsmb.2890;
RA Hendriks I.A., D'Souza R.C., Yang B., Verlaan-de Vries M., Mann M.,
RA Vertegaal A.C.;
RT "Uncovering global SUMOylation signaling networks in a site-specific
RT manner.";
RL Nat. Struct. Mol. Biol. 21:927-936(2014).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6AW86-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6AW86-2; Sequence=VSP_023658;
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AK127750; BAC87113.1; -; mRNA.
DR EMBL; AK131452; BAD18597.1; -; mRNA.
DR EMBL; BX648711; CAH10556.1; -; mRNA.
DR EMBL; BC140881; AAI40882.1; -; mRNA.
DR EMBL; BC140882; AAI40883.1; -; mRNA.
DR CCDS; CCDS33138.1; -. [Q6AW86-1]
DR RefSeq; NP_997278.2; NM_207395.2. [Q6AW86-1]
DR RefSeq; XP_005258972.1; XM_005258915.4. [Q6AW86-1]
DR RefSeq; XP_005258975.1; XM_005258918.4. [Q6AW86-1]
DR AlphaFoldDB; Q6AW86; -.
DR SMR; Q6AW86; -.
DR BioGRID; 132751; 86.
DR ELM; Q6AW86; -.
DR IntAct; Q6AW86; 79.
DR STRING; 9606.ENSP00000337473; -.
DR iPTMnet; Q6AW86; -.
DR PhosphoSitePlus; Q6AW86; -.
DR BioMuta; ZNF324B; -.
DR DMDM; 74757410; -.
DR jPOST; Q6AW86; -.
DR MassIVE; Q6AW86; -.
DR MaxQB; Q6AW86; -.
DR PaxDb; Q6AW86; -.
DR PeptideAtlas; Q6AW86; -.
DR PRIDE; Q6AW86; -.
DR ProteomicsDB; 66190; -. [Q6AW86-1]
DR ProteomicsDB; 66191; -. [Q6AW86-2]
DR Antibodypedia; 52700; 61 antibodies from 14 providers.
DR DNASU; 388569; -.
DR Ensembl; ENST00000336614.9; ENSP00000337473.3; ENSG00000249471.8. [Q6AW86-1]
DR Ensembl; ENST00000545523.5; ENSP00000438930.1; ENSG00000249471.8. [Q6AW86-1]
DR GeneID; 388569; -.
DR KEGG; hsa:388569; -.
DR MANE-Select; ENST00000336614.9; ENSP00000337473.3; NM_207395.3; NP_997278.2.
DR UCSC; uc002qsv.2; human. [Q6AW86-1]
DR CTD; 388569; -.
DR DisGeNET; 388569; -.
DR GeneCards; ZNF324B; -.
DR HGNC; HGNC:33107; ZNF324B.
DR HPA; ENSG00000249471; Low tissue specificity.
DR neXtProt; NX_Q6AW86; -.
DR OpenTargets; ENSG00000249471; -.
DR PharmGKB; PA162410082; -.
DR VEuPathDB; HostDB:ENSG00000249471; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000163047; -.
DR HOGENOM; CLU_002678_0_2_1; -.
DR InParanoid; Q6AW86; -.
DR OMA; AAWHEPQ; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q6AW86; -.
DR TreeFam; TF337574; -.
DR PathwayCommons; Q6AW86; -.
DR Reactome; R-HSA-212436; Generic Transcription Pathway.
DR SignaLink; Q6AW86; -.
DR BioGRID-ORCS; 388569; 15 hits in 1098 CRISPR screens.
DR GenomeRNAi; 388569; -.
DR Pharos; Q6AW86; Tdark.
DR PRO; PR:Q6AW86; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q6AW86; protein.
DR Bgee; ENSG00000249471; Expressed in cortical plate and 94 other tissues.
DR ExpressionAtlas; Q6AW86; baseline and differential.
DR Genevisible; Q6AW86; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 8.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 9.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 5.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 9.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 9.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..544
FT /note="Zinc finger protein 324B"
FT /id="PRO_0000280405"
FT DOMAIN 1..72
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 257..279
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 285..307
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 313..335
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 341..363
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 369..391
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 397..419
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 425..447
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 453..475
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 481..503
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 184..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 508..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 214
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:25218447"
FT VAR_SEQ 1..79
FT /note="MTFEDVAVYFSQEEWGLLDTAQRALYRHVMLENFTLVTSLGLSTSRPRVVIQ
FT LERGEEPWVPSGKDMTLARNTYGRLNS -> MGVPTAVSATPVRADASSKPQPLLQSQP
FT HLFFFPKLLSRLLGSPLPVHSAGPGPLLTRMPQATTVSLRL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_023658"
FT VARIANT 63
FT /note="S -> G (in dbSNP:rs12611254)"
FT /id="VAR_052811"
FT CONFLICT 402
FT /note="C -> R (in Ref. 1; BAD18597)"
FT /evidence="ECO:0000305"
FT CONFLICT 457
FT /note="D -> G (in Ref. 1; BAC87113)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 544 AA; 60602 MW; 7CC2D443961CA98D CRC64;
MTFEDVAVYF SQEEWGLLDT AQRALYRHVM LENFTLVTSL GLSTSRPRVV IQLERGEEPW
VPSGKDMTLA RNTYGRLNSG SWSLTEDRDV SGEWPRAFPD TPPGMTTSVF PVADACHSVK
SLQRQPGASP SQERKPTGVS VIYWERLLLG SRSDQASISL RLTSPLRPPK SSRPREKTFT
EYRVPGRQPR TPERQKPCAQ EVPGRAFGNA SDLKAASGGR DRRMGAAWQE PHRLLGGQEP
STWDELGEAL HAGEKSFECR ACSKVFVKSS DLLKHLRTHT GERPYECTQC GKAFSQTSHL
TQHQRIHSGE TPYACPVCGK AFRHSSSLVR HQRIHTAEKS FRCSECGKAF SHGSNLSQHR
KIHAGGRPYA CAQCGRRFCR NSHLIQHERT HTGEKPFVCA LCGAAFSQGS SLFLHQRVHT
GEKPFACAQC GRSFSRSSNL TQHQLLHTGE RPFRCVDCGK GFAKGAVLLS HRRIHTGEKP
FVCTQCGRAF RERPALLHHQ RIHTTEKTNA AAPDCTPGPG FLQGHHRKVR RGGKPSPVLK
PAKV
