Z512B_HUMAN
ID Z512B_HUMAN Reviewed; 892 AA.
AC Q96KM6; Q08AK9; Q9ULM4;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Zinc finger protein 512B;
GN Name=ZNF512B; Synonyms=KIAA1196;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 42-892.
RC TISSUE=Brain;
RX PubMed=10574462; DOI=10.1093/dnares/6.5.337;
RA Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XV. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 6:337-345(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 425-892.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026;
RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
RT "Global, in vivo, and site-specific phosphorylation dynamics in signaling
RT networks.";
RL Cell 127:635-648(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-686, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [8]
RP STRUCTURE BY NMR OF 493-577.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the two ZF-C2H2-like domains (493-575) of human zinc
RT finger protein KIAA1196.";
RL Submitted (OCT-2006) to the PDB data bank.
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q96KM6; Q96IF1: AJUBA; NbExp=3; IntAct=EBI-1049952, EBI-949782;
CC Q96KM6; Q8N9N5-2: BANP; NbExp=3; IntAct=EBI-1049952, EBI-11524452;
CC Q96KM6; Q9H3H3-3: C11orf68; NbExp=3; IntAct=EBI-1049952, EBI-12002214;
CC Q96KM6; Q13643: FHL3; NbExp=8; IntAct=EBI-1049952, EBI-741101;
CC Q96KM6; Q08379: GOLGA2; NbExp=3; IntAct=EBI-1049952, EBI-618309;
CC Q96KM6; Q99750: MDFI; NbExp=3; IntAct=EBI-1049952, EBI-724076;
CC Q96KM6; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-1049952, EBI-16439278;
CC Q96KM6; Q5JR59-3: MTUS2; NbExp=3; IntAct=EBI-1049952, EBI-11522433;
CC Q96KM6; Q96RE7: NACC1; NbExp=3; IntAct=EBI-1049952, EBI-7950997;
CC Q96KM6; Q15276: RABEP1; NbExp=3; IntAct=EBI-1049952, EBI-447043;
CC Q96KM6; Q8IUQ4: SIAH1; NbExp=3; IntAct=EBI-1049952, EBI-747107;
CC Q96KM6; Q13077: TRAF1; NbExp=7; IntAct=EBI-1049952, EBI-359224;
CC Q96KM6; P52747: ZNF143; NbExp=3; IntAct=EBI-1049952, EBI-2849334;
CC Q96KM6; Q15942: ZYX; NbExp=3; IntAct=EBI-1049952, EBI-444225;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AL118506; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC125128; AAI25129.1; -; mRNA.
DR EMBL; BC125129; AAI25130.1; -; mRNA.
DR EMBL; AB033022; BAA86510.1; -; mRNA.
DR EMBL; AL834525; CAD39181.1; -; mRNA.
DR CCDS; CCDS13548.1; -.
DR RefSeq; NP_065764.1; NM_020713.2.
DR RefSeq; XP_011527231.1; XM_011528929.2.
DR PDB; 2GQJ; NMR; -; A=493-577.
DR PDBsum; 2GQJ; -.
DR AlphaFoldDB; Q96KM6; -.
DR SMR; Q96KM6; -.
DR BioGRID; 121543; 179.
DR IntAct; Q96KM6; 101.
DR MINT; Q96KM6; -.
DR STRING; 9606.ENSP00000393795; -.
DR GlyGen; Q96KM6; 6 sites, 2 O-linked glycans (6 sites).
DR iPTMnet; Q96KM6; -.
DR PhosphoSitePlus; Q96KM6; -.
DR BioMuta; ZNF512B; -.
DR DMDM; 23822331; -.
DR EPD; Q96KM6; -.
DR jPOST; Q96KM6; -.
DR MassIVE; Q96KM6; -.
DR MaxQB; Q96KM6; -.
DR PaxDb; Q96KM6; -.
DR PeptideAtlas; Q96KM6; -.
DR PRIDE; Q96KM6; -.
DR ProteomicsDB; 77083; -.
DR Antibodypedia; 29948; 108 antibodies from 17 providers.
DR DNASU; 57473; -.
DR Ensembl; ENST00000369888.6; ENSP00000358904.1; ENSG00000196700.9.
DR GeneID; 57473; -.
DR KEGG; hsa:57473; -.
DR MANE-Select; ENST00000369888.6; ENSP00000358904.1; NM_020713.3; NP_065764.1.
DR UCSC; uc002yhl.3; human.
DR CTD; 57473; -.
DR DisGeNET; 57473; -.
DR GeneCards; ZNF512B; -.
DR HGNC; HGNC:29212; ZNF512B.
DR HPA; ENSG00000196700; Low tissue specificity.
DR MIM; 617886; gene.
DR neXtProt; NX_Q96KM6; -.
DR OpenTargets; ENSG00000196700; -.
DR PharmGKB; PA162410181; -.
DR VEuPathDB; HostDB:ENSG00000196700; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000159165; -.
DR HOGENOM; CLU_020005_1_0_1; -.
DR InParanoid; Q96KM6; -.
DR OMA; LAGKYHC; -.
DR OrthoDB; 222563at2759; -.
DR PhylomeDB; Q96KM6; -.
DR TreeFam; TF331185; -.
DR PathwayCommons; Q96KM6; -.
DR Reactome; R-HSA-9013424; RHOV GTPase cycle.
DR SignaLink; Q96KM6; -.
DR BioGRID-ORCS; 57473; 23 hits in 1098 CRISPR screens.
DR ChiTaRS; ZNF512B; human.
DR EvolutionaryTrace; Q96KM6; -.
DR GenomeRNAi; 57473; -.
DR Pharos; Q96KM6; Tbio.
DR PRO; PR:Q96KM6; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q96KM6; protein.
DR Bgee; ENSG00000196700; Expressed in parotid gland and 188 other tissues.
DR Genevisible; Q96KM6; HS.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:ARUK-UCL.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:ARUK-UCL.
DR GO; GO:1902894; P:negative regulation of miRNA transcription; IDA:ARUK-UCL.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 6.
DR SUPFAM; SSF57667; SSF57667; 7.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..892
FT /note="Zinc finger protein 512B"
FT /id="PRO_0000047779"
FT ZN_FING 105..129
FT /note="C2H2-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 140..163
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 540..563
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 594..618
FT /note="C2H2-type 4; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 630..653
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 750..774
FT /note="C2H2-type 6; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 784..807
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 323..473
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 562..582
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 649..682
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 812..892
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..81
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..393
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 447..469
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 564..582
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 649..677
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 847..879
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 409
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17081983"
FT MOD_RES 686
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT VARIANT 288
FT /note="V -> M (in dbSNP:rs45486695)"
FT /id="VAR_061954"
FT VARIANT 372
FT /note="M -> V (in dbSNP:rs817326)"
FT /id="VAR_024226"
FT VARIANT 453
FT /note="A -> T (in dbSNP:rs6062599)"
FT /id="VAR_024227"
FT HELIX 499..502
FT /evidence="ECO:0007829|PDB:2GQJ"
FT TURN 503..507
FT /evidence="ECO:0007829|PDB:2GQJ"
FT TURN 513..515
FT /evidence="ECO:0007829|PDB:2GQJ"
FT HELIX 524..540
FT /evidence="ECO:0007829|PDB:2GQJ"
FT STRAND 543..545
FT /evidence="ECO:0007829|PDB:2GQJ"
FT HELIX 552..562
FT /evidence="ECO:0007829|PDB:2GQJ"
SQ SEQUENCE 892 AA; 97264 MW; 50449C476DFCE4DF CRC64;
MTDPFCVGGR RLPGSSKSGP GKDGSRKEVR LPMLHDPPKM GMPVVRGGQT VPGQAPLCFD
PGSPASDKTE GKKKGRPKAE NQALRDIPLS LMNDWKDEFK AHSRVKCPNS GCWLEFPSIY
GLKYHYQRCQ GGAISDRLAF PCPFCEAAFT SKTQLEKHRI WNHMDRPLPA SKPGPISRPV
TISRPVGVSK PIGVSKPVTI GKPVGVSKPI GISKPVSVGR PMPVTKAIPV TRPVPVTKPV
TVSRPMPVTK AMPVTKPITV TKSVPVTKPV PVTKPITVTK LVTVTKPVPV TKPVTVSRPI
VVSKPVTVSR PIAISRHTPP CKMVLLTRSE NKAPRATGRN SGKKRAADSL DTCPIPPKQA
RPENGEYGPS SMGQSSAFQL SADTSSGSLS PGSRPSGGME ALKAAGPASP PEEDPERTKH
RRKQKTPKKF TGEQPSISGT FGLKGLVKAE DKARVHRSKK QEGPGPEDAR KKVPAAPITV
SKEAPAPVAH PAPGGPEEQW QRAIHERGEA VCPTCNVVTR KTLVGLKKHM EVCQKLQDAL
KCQHCRKQFK SKAGLNYHTM AEHSAKPSDA EASEGGEQEE RERLRKVLKQ MGRLRCPQEG
CGAAFSSLMG YQYHQRRCGK PPCEVDSPSF PCTHCGKTYR SKAGHDYHVR SEHTAPPPEE
PTDKSPEAED PLGVERTPSG RVRRTSAQVA VFHLQEIAED ELARDWTKRR MKDDLVPETA
RLNYTRPGLP TLNPQLLEAW KNEVKEKGHV NCPNDCCEAI YSSVSGLKAH LASCSKGAHL
AGKYRCLLCP KEFSSESGVK YHILKTHAEN WFRTSADPPP KHRSQDSLVP KKEKKKNLAG
GKKRGRKPKE RTPEEPVAKL PPRRDDWPPG CRDKGARGST GRKVGVSKAP EK
