ZAPD_ECOLI
ID ZAPD_ECOLI Reviewed; 247 AA.
AC P36680; P75645; Q8KJQ6; Q8KMZ7;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Cell division protein ZapD {ECO:0000255|HAMAP-Rule:MF_01092};
DE AltName: Full=Z ring-associated protein D {ECO:0000255|HAMAP-Rule:MF_01092};
GN Name=zapD {ECO:0000255|HAMAP-Rule:MF_01092}; Synonyms=yacF;
GN OrderedLocusNames=b0102, JW0099;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8202364; DOI=10.1093/nar/22.9.1637;
RA Fujita N., Mori H., Yura T., Ishihama A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-
RT 4.1 min (110,917-193,643 bp) region.";
RL Nucleic Acids Res. 22:1637-1639(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, SUBUNIT, INTERACTION WITH FTSZ, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND GENE NAME.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=22505682; DOI=10.1128/jb.00176-12;
RA Durand-Heredia J., Rivkin E., Fan G., Morales J., Janakiraman A.;
RT "Identification of ZapD as a cell division factor that promotes the
RT assembly of FtsZ in Escherichia coli.";
RL J. Bacteriol. 194:3189-3198(2012).
CC -!- FUNCTION: Cell division factor that enhances FtsZ-ring assembly.
CC Directly interacts with FtsZ and promotes bundling of FtsZ
CC protofilaments, with a reduction in FtsZ GTPase activity.
CC {ECO:0000255|HAMAP-Rule:MF_01092, ECO:0000269|PubMed:22505682}.
CC -!- SUBUNIT: Mostly homodimer in solution. Interacts with the C-terminal
CC tail of FtsZ. {ECO:0000269|PubMed:22505682}.
CC -!- INTERACTION:
CC P36680; P0A9A6: ftsZ; NbExp=3; IntAct=EBI-1113728, EBI-370963;
CC P36680; P36680: zapD; NbExp=2; IntAct=EBI-1113728, EBI-1113728;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01092,
CC ECO:0000269|PubMed:22505682}. Note=Localizes to mid-cell in an FtsZ-
CC dependent manner. Localization does not require FtsA, ZipA, ZapA or
CC ZapC.
CC -!- DISRUPTION PHENOTYPE: Mutants are viable, with no discernible division
CC phenotypes. {ECO:0000269|PubMed:22505682}.
CC -!- SIMILARITY: Belongs to the ZapD family. {ECO:0000255|HAMAP-
CC Rule:MF_01092}.
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DR EMBL; U00096; AAC73213.1; -; Genomic_DNA.
DR EMBL; AP009048; BAB96671.2; -; Genomic_DNA.
DR PIR; F64732; F64732.
DR RefSeq; NP_414644.1; NC_000913.3.
DR RefSeq; WP_001194734.1; NZ_STEB01000010.1.
DR PDB; 5DKO; X-ray; 2.40 A; A=1-247.
DR PDB; 5IMJ; X-ray; 3.10 A; A/B=2-247.
DR PDB; 5KOA; X-ray; 2.67 A; A/B=2-247.
DR PDBsum; 5DKO; -.
DR PDBsum; 5IMJ; -.
DR PDBsum; 5KOA; -.
DR AlphaFoldDB; P36680; -.
DR SMR; P36680; -.
DR BioGRID; 4260784; 3.
DR IntAct; P36680; 5.
DR STRING; 511145.b0102; -.
DR jPOST; P36680; -.
DR PaxDb; P36680; -.
DR PRIDE; P36680; -.
DR EnsemblBacteria; AAC73213; AAC73213; b0102.
DR EnsemblBacteria; BAB96671; BAB96671; BAB96671.
DR GeneID; 66671609; -.
DR GeneID; 944873; -.
DR KEGG; ecj:JW0099; -.
DR KEGG; eco:b0102; -.
DR PATRIC; fig|1411691.4.peg.2179; -.
DR EchoBASE; EB2219; -.
DR eggNOG; COG4582; Bacteria.
DR HOGENOM; CLU_076303_0_0_6; -.
DR OMA; LPAYYAW; -.
DR PhylomeDB; P36680; -.
DR BioCyc; EcoCyc:EG12313-MON; -.
DR PRO; PR:P36680; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0032153; C:cell division site; IDA:EcoCyc.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000917; P:division septum assembly; IEA:UniProtKB-KW.
DR GO; GO:0043093; P:FtsZ-dependent cytokinesis; IMP:EcoCyc.
DR Gene3D; 2.60.440.10; -; 1.
DR HAMAP; MF_01092; ZapD; 1.
DR InterPro; IPR009777; ZapD.
DR InterPro; IPR027462; ZapD_C.
DR InterPro; IPR036268; ZapD_sf.
DR PANTHER; PTHR39455; PTHR39455; 1.
DR Pfam; PF07072; ZapD; 1.
DR SUPFAM; SSF160950; SSF160950; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Cytoplasm; Reference proteome;
KW Septation.
FT CHAIN 1..247
FT /note="Cell division protein ZapD"
FT /id="PRO_0000211667"
FT CONFLICT 215
FT /note="I -> T (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT STRAND 6..12
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 13..28
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 37..55
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 59..76
FT /evidence="ECO:0007829|PDB:5DKO"
FT STRAND 78..80
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 85..104
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 110..114
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 116..124
FT /evidence="ECO:0007829|PDB:5DKO"
FT TURN 128..131
FT /evidence="ECO:0007829|PDB:5DKO"
FT TURN 133..135
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 137..142
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 147..159
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 162..175
FT /evidence="ECO:0007829|PDB:5DKO"
FT STRAND 182..187
FT /evidence="ECO:0007829|PDB:5DKO"
FT STRAND 190..194
FT /evidence="ECO:0007829|PDB:5DKO"
FT STRAND 199..204
FT /evidence="ECO:0007829|PDB:5DKO"
FT HELIX 207..209
FT /evidence="ECO:0007829|PDB:5DKO"
FT STRAND 211..218
FT /evidence="ECO:0007829|PDB:5DKO"
FT STRAND 221..233
FT /evidence="ECO:0007829|PDB:5DKO"
FT STRAND 240..246
FT /evidence="ECO:0007829|PDB:5DKO"
SQ SEQUENCE 247 AA; 28292 MW; 3C0B917A7FA844AC CRC64;
MQTQVLFEHP LNEKMRTWLR IEFLIQQLTV NLPIVDHAGA LHFFRNVSEL LDVFERGEVR
TELLKELDRQ QRKLQTWIGV PGVDQSRIEA LIQQLKAAGS VLISAPRIGQ FLREDRLIAL
VRQRLSIPGG CCSFDLPTLH IWLHLPQAQR DSQVETWIAS LNPLTQALTM VLDLIRQSAP
FRKQTSLNGF YQDNGGDADL LRLNLSLDSQ LYPQISGHKS RFAIRFMPLD TENGQVPERL
DFELACC
