ZBT8B_HUMAN
ID ZBT8B_HUMAN Reviewed; 495 AA.
AC Q8NAP8; Q15DG5; Q5VXR5; Q69YT7;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Zinc finger and BTB domain-containing protein 8B;
GN Name=ZBTB8B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 179-495 (ISOFORM 2).
RC TISSUE=Testis;
RA Alliel P.M., Perin J.P., Goudou D.;
RT "Cloning, expression and functional characterization of B-twin cDNA.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 380-467 (ISOFORMS 1/2).
RC TISSUE=Brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP VARIANT [LARGE SCALE ANALYSIS] LYS-181.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q8NAP8; O00311: CDC7; NbExp=3; IntAct=EBI-17494306, EBI-374980;
CC Q8NAP8; Q68EN5: KIAA0895L; NbExp=3; IntAct=EBI-17494306, EBI-17494528;
CC Q8NAP8; P25800: LMO1; NbExp=3; IntAct=EBI-17494306, EBI-8639312;
CC Q8NAP8; Q8TAP4-4: LMO3; NbExp=3; IntAct=EBI-17494306, EBI-11742507;
CC Q8NAP8; Q9UBU8-2: MORF4L1; NbExp=3; IntAct=EBI-17494306, EBI-10288852;
CC Q8NAP8; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-17494306, EBI-741158;
CC Q8NAP8; O43765: SGTA; NbExp=3; IntAct=EBI-17494306, EBI-347996;
CC Q8NAP8; Q9H0A9-2: SPATC1L; NbExp=3; IntAct=EBI-17494306, EBI-11995806;
CC Q8NAP8; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-17494306, EBI-741480;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8NAP8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8NAP8-2; Sequence=VSP_039858;
CC -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
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DR EMBL; AK092326; BAC03863.1; -; mRNA.
DR EMBL; AL033529; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL356986; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DQ500954; ABF68616.1; -; mRNA.
DR EMBL; AL442095; CAH10660.1; -; mRNA.
DR CCDS; CCDS44104.1; -. [Q8NAP8-1]
DR RefSeq; NP_001139192.1; NM_001145720.1. [Q8NAP8-1]
DR AlphaFoldDB; Q8NAP8; -.
DR SMR; Q8NAP8; -.
DR BioGRID; 608552; 16.
DR IntAct; Q8NAP8; 9.
DR STRING; 9606.ENSP00000476499; -.
DR iPTMnet; Q8NAP8; -.
DR PhosphoSitePlus; Q8NAP8; -.
DR BioMuta; ZBTB8B; -.
DR DMDM; 308153528; -.
DR MassIVE; Q8NAP8; -.
DR PaxDb; Q8NAP8; -.
DR PeptideAtlas; Q8NAP8; -.
DR PRIDE; Q8NAP8; -.
DR ProteomicsDB; 72689; -. [Q8NAP8-1]
DR ProteomicsDB; 72690; -. [Q8NAP8-2]
DR Antibodypedia; 71786; 91 antibodies from 17 providers.
DR DNASU; 728116; -.
DR Ensembl; ENST00000609129.2; ENSP00000476499.1; ENSG00000273274.2. [Q8NAP8-1]
DR GeneID; 728116; -.
DR KEGG; hsa:728116; -.
DR MANE-Select; ENST00000609129.2; ENSP00000476499.1; NM_001145720.2; NP_001139192.1.
DR UCSC; uc001bvl.5; human. [Q8NAP8-1]
DR CTD; 728116; -.
DR DisGeNET; 728116; -.
DR GeneCards; ZBTB8B; -.
DR HGNC; HGNC:37057; ZBTB8B.
DR HPA; ENSG00000273274; Tissue enhanced (brain).
DR neXtProt; NX_Q8NAP8; -.
DR OpenTargets; ENSG00000254553; -.
DR PharmGKB; PA164727627; -.
DR VEuPathDB; HostDB:ENSG00000273274; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000154994; -.
DR HOGENOM; CLU_022356_0_1_1; -.
DR InParanoid; Q8NAP8; -.
DR OMA; ICNRERD; -.
DR OrthoDB; 755439at2759; -.
DR PhylomeDB; Q8NAP8; -.
DR TreeFam; TF330979; -.
DR PathwayCommons; Q8NAP8; -.
DR SignaLink; Q8NAP8; -.
DR BioGRID-ORCS; 728116; 10 hits in 1071 CRISPR screens.
DR ChiTaRS; ZBTB8B; human.
DR GenomeRNAi; 728116; -.
DR Pharos; Q8NAP8; Tdark.
DR PRO; PR:Q8NAP8; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q8NAP8; protein.
DR Bgee; ENSG00000273274; Expressed in buccal mucosa cell and 29 other tissues.
DR Genevisible; Q8NAP8; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..495
FT /note="Zinc finger and BTB domain-containing protein 8B"
FT /id="PRO_0000047721"
FT DOMAIN 24..92
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT ZN_FING 341..363
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 369..392
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 157..178
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 446..495
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 468..495
FT /note="GEENDPAGDDSDDKPQIQPNLSDRETLT -> EIGSHYVAQASLELLALSQP
FT PASASQSAGITDGVSLCCPGRSTVA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039, ECO:0000303|Ref.3"
FT /id="VSP_039858"
FT VARIANT 181
FT /note="E -> K (in a colorectal cancer sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_035602"
SQ SEQUENCE 495 AA; 54175 MW; B5139F634D10C8C9 CRC64;
MEMQSYYAKL LGELNEQRKR DFFCDCSIIV EGRIFKAHRN ILFANSGYFR ALLIHYIQDS
GRHSTASLDI VTSDAFSIIL DFLYSGKLDL CGENVIEVMS AASYLQMNDV VNFCKTYIRS
SLDICRKMEK EAAVAAAVAA AAAAAAAAAA AAAHQVDSES PSSGREGTSC GTKSLVSSPA
EGEKSVECLR ESPCGDCGDC HPLELVVRDS LGGGSADSNL STPPKRIEPK VEFDADEVEV
DVGEQLQQYA APLNLAHVEE ALPSGQAVDL AYSNYHVKQF LEALLRNSAA PSKDDADHHF
SRSLEGRPEG AGVAMSSMMD VQADWYGEDS GDVLVVPIKL HKCPFCPYTA KQKGILKRHI
RSHTGERPYP CETCGKRFTR QEHLRSHALS VHRSNRPIIC KGCRRTFTSH LSQGLRRFGL
CDSCTCVTDT PDDDDDLMPI NLSLVEASSE SQEKSDTDND WPIYVESGEE NDPAGDDSDD
KPQIQPNLSD RETLT
