ZBTB9_HUMAN
ID ZBTB9_HUMAN Reviewed; 473 AA.
AC Q96C00; A2AB19;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Zinc finger and BTB domain-containing protein 9;
GN Name=ZBTB9;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-286, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25218447; DOI=10.1038/nsmb.2890;
RA Hendriks I.A., D'Souza R.C., Yang B., Verlaan-de Vries M., Mann M.,
RA Vertegaal A.C.;
RT "Uncovering global SUMOylation signaling networks in a site-specific
RT manner.";
RL Nat. Struct. Mol. Biol. 21:927-936(2014).
RN [7]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-286, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25114211; DOI=10.1073/pnas.1413825111;
RA Impens F., Radoshevich L., Cossart P., Ribet D.;
RT "Mapping of SUMO sites and analysis of SUMOylation changes induced by
RT external stimuli.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:12432-12437(2014).
RN [8]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-286, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25772364; DOI=10.1016/j.celrep.2015.02.033;
RA Hendriks I.A., Treffers L.W., Verlaan-de Vries M., Olsen J.V.,
RA Vertegaal A.C.;
RT "SUMO-2 orchestrates chromatin modifiers in response to DNA damage.";
RL Cell Rep. 10:1778-1791(2015).
RN [9]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-286, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25755297; DOI=10.1074/mcp.o114.044792;
RA Xiao Z., Chang J.G., Hendriks I.A., Sigurdsson J.O., Olsen J.V.,
RA Vertegaal A.C.;
RT "System-wide analysis of SUMOylation dynamics in response to replication
RT stress reveals novel small ubiquitin-like modified target proteins and
RT acceptor lysines relevant for genome stability.";
RL Mol. Cell. Proteomics 14:1419-1434(2015).
RN [10]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-286; LYS-293; LYS-307 AND
RP LYS-382, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q96C00; Q7Z479: CAPN7; NbExp=3; IntAct=EBI-395708, EBI-10213454;
CC Q96C00; Q9HC52: CBX8; NbExp=3; IntAct=EBI-395708, EBI-712912;
CC Q96C00; Q8N8U2: CDYL2; NbExp=3; IntAct=EBI-395708, EBI-8467076;
CC Q96C00; A0PJX0: CIB4; NbExp=3; IntAct=EBI-395708, EBI-12868028;
CC Q96C00; P61024: CKS1B; NbExp=3; IntAct=EBI-395708, EBI-456371;
CC Q96C00; O60941: DTNB; NbExp=5; IntAct=EBI-395708, EBI-740402;
CC Q96C00; Q92630: DYRK2; NbExp=3; IntAct=EBI-395708, EBI-749432;
CC Q96C00; Q08426: EHHADH; NbExp=6; IntAct=EBI-395708, EBI-2339219;
CC Q96C00; O60573: EIF4E2; NbExp=4; IntAct=EBI-395708, EBI-398610;
CC Q96C00; Q07627: KRTAP1-1; NbExp=3; IntAct=EBI-395708, EBI-11959885;
CC Q96C00; P60409: KRTAP10-7; NbExp=7; IntAct=EBI-395708, EBI-10172290;
CC Q96C00; P60411: KRTAP10-9; NbExp=6; IntAct=EBI-395708, EBI-10172052;
CC Q96C00; P59991: KRTAP12-2; NbExp=6; IntAct=EBI-395708, EBI-10176379;
CC Q96C00; Q14847: LASP1; NbExp=4; IntAct=EBI-395708, EBI-742828;
CC Q96C00; Q14566: MCM6; NbExp=3; IntAct=EBI-395708, EBI-374900;
CC Q96C00; Q99750: MDFI; NbExp=6; IntAct=EBI-395708, EBI-724076;
CC Q96C00; Q9UBU8: MORF4L1; NbExp=4; IntAct=EBI-395708, EBI-399246;
CC Q96C00; Q9UBU8-2: MORF4L1; NbExp=3; IntAct=EBI-395708, EBI-10288852;
CC Q96C00; Q13526: PIN1; NbExp=6; IntAct=EBI-395708, EBI-714158;
CC Q96C00; Q9Y237: PIN4; NbExp=4; IntAct=EBI-395708, EBI-714599;
CC Q96C00; Q8TAD8: SNIP1; NbExp=6; IntAct=EBI-395708, EBI-749336;
CC Q96C00; Q96C24: SYTL4; NbExp=3; IntAct=EBI-395708, EBI-747142;
CC Q96C00; Q15560: TCEA2; NbExp=3; IntAct=EBI-395708, EBI-710310;
CC Q96C00; Q8N8B7-2: TCEANC; NbExp=3; IntAct=EBI-395708, EBI-11955057;
CC Q96C00; Q6FI91: TSPYL; NbExp=3; IntAct=EBI-395708, EBI-723389;
CC Q96C00; Q969E8: TSR2; NbExp=6; IntAct=EBI-395708, EBI-746981;
CC Q96C00; Q7KZS0: UBE2I; NbExp=3; IntAct=EBI-395708, EBI-10180829;
CC Q96C00; O43167: ZBTB24; NbExp=3; IntAct=EBI-395708, EBI-744471;
CC Q96C00; Q9HCK0: ZBTB26; NbExp=3; IntAct=EBI-395708, EBI-3918996;
CC Q96C00; P10074: ZBTB48; NbExp=3; IntAct=EBI-395708, EBI-744864;
CC Q96C00; Q96C00: ZBTB9; NbExp=5; IntAct=EBI-395708, EBI-395708;
CC Q96C00; Q8TAU3: ZNF417; NbExp=3; IntAct=EBI-395708, EBI-740727;
CC Q96C00; Q9NQZ8: ZNF71; NbExp=3; IntAct=EBI-395708, EBI-7138235;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; AK122644; BAG53637.1; -; mRNA.
DR EMBL; BX088650; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471081; EAX03739.1; -; Genomic_DNA.
DR EMBL; BC014978; AAH14978.1; -; mRNA.
DR CCDS; CCDS4780.1; -.
DR RefSeq; NP_689948.1; NM_152735.3.
DR AlphaFoldDB; Q96C00; -.
DR SMR; Q96C00; -.
DR BioGRID; 128736; 106.
DR IntAct; Q96C00; 72.
DR MINT; Q96C00; -.
DR STRING; 9606.ENSP00000378503; -.
DR iPTMnet; Q96C00; -.
DR PhosphoSitePlus; Q96C00; -.
DR BioMuta; ZBTB9; -.
DR DMDM; 46577533; -.
DR EPD; Q96C00; -.
DR jPOST; Q96C00; -.
DR MassIVE; Q96C00; -.
DR MaxQB; Q96C00; -.
DR PaxDb; Q96C00; -.
DR PeptideAtlas; Q96C00; -.
DR PRIDE; Q96C00; -.
DR ProteomicsDB; 76137; -.
DR Antibodypedia; 14338; 99 antibodies from 22 providers.
DR DNASU; 221504; -.
DR Ensembl; ENST00000395064.3; ENSP00000378503.2; ENSG00000213588.6.
DR Ensembl; ENST00000414934.2; ENSP00000413557.2; ENSG00000236515.3.
DR GeneID; 221504; -.
DR KEGG; hsa:221504; -.
DR MANE-Select; ENST00000395064.3; ENSP00000378503.2; NM_152735.4; NP_689948.1.
DR UCSC; uc003oeq.4; human.
DR CTD; 221504; -.
DR DisGeNET; 221504; -.
DR GeneCards; ZBTB9; -.
DR HGNC; HGNC:28323; ZBTB9.
DR HPA; ENSG00000213588; Low tissue specificity.
DR neXtProt; NX_Q96C00; -.
DR OpenTargets; ENSG00000213588; -.
DR PharmGKB; PA134895172; -.
DR VEuPathDB; HostDB:ENSG00000213588; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162408; -.
DR HOGENOM; CLU_029118_2_0_1; -.
DR InParanoid; Q96C00; -.
DR OMA; KVFYIKQ; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q96C00; -.
DR PathwayCommons; Q96C00; -.
DR SignaLink; Q96C00; -.
DR BioGRID-ORCS; 221504; 18 hits in 1116 CRISPR screens.
DR GenomeRNAi; 221504; -.
DR Pharos; Q96C00; Tdark.
DR PRO; PR:Q96C00; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q96C00; protein.
DR Bgee; ENSG00000213588; Expressed in pituitary gland and 103 other tissues.
DR ExpressionAtlas; Q96C00; baseline and differential.
DR Genevisible; Q96C00; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation; Ubl conjugation; Zinc;
KW Zinc-finger.
FT CHAIN 1..473
FT /note="Zinc finger and BTB domain-containing protein 9"
FT /id="PRO_0000047723"
FT DOMAIN 48..112
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT ZN_FING 411..433
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 438..460
FT /note="C2H2-type 2; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 177..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 293..376
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 177..195
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..227
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 286
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO1); alternate"
FT /evidence="ECO:0007744|PubMed:25114211"
FT CROSSLNK 286
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2); alternate"
FT /evidence="ECO:0007744|PubMed:25218447,
FT ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:25772364,
FT ECO:0007744|PubMed:28112733"
FT CROSSLNK 293
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 307
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 382
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VARIANT 274
FT /note="A -> G (in dbSNP:rs9469425)"
FT /id="VAR_061981"
SQ SEQUENCE 473 AA; 50602 MW; 2BC8041790090FD9 CRC64;
METPTPLPPV PASPTCNPAP RTIQIEFPQH SSSLLESLNR HRLEGKFCDV SLLVQGRELR
AHKAVLAAAS PYFHDKLLLG DAPRLTLPSV IEADAFEGLL QLIYSGRLRL PLDALPAHLL
VASGLQMWQV VDQCSEILRE LETSGGGISA RGGNSYHALL STTSSTGGWC IRSSPFQTPV
QSSASTESPA STESPVGGEG SELGEVLQIQ VEEEEEEEED DDDEDQGSAT LSQTPQPQRV
SGVFPRPHGP HPLPMTATPR KLPEGESAPL ELPAPPALPP KIFYIKQEPF EPKEEISGSG
TQPGGAKEET KVFSGGDTEG NGELGFLLPS GPGPTSGGGG PSWKPVDLHG NEILSGGGGP
GGAGQAVHGP VKLGGTPPAD GKRFGCLCGK RFAVKPKRDR HIMLTFSLRP FGCGICNKRF
KLKHHLTEHM KTHAGALHAC PHCGRRFRVH ACFLRHRDLC KGQGWATAHW TYK
