ZC321_TRYB2
ID ZC321_TRYB2 Reviewed; 461 AA.
AC Q57Y77; D6XKI4;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=RNA-binding protein ZCH321 {ECO:0000305};
DE AltName: Full=CCCH zinc finger protein ZC3H21 {ECO:0000305};
GN Name=ZCH321 {ECO:0000303|PubMed:31743541};
GN ORFNames=Tb927.7.2670 {ECO:0000312|EMBL:AAX69418.1};
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431 {ECO:0000312|Proteomes:UP000008524};
RN [1] {ECO:0000312|EMBL:AAZ12331.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000312|EMBL:AAZ12331.1};
RX PubMed=16020724; DOI=10.1126/science.1112181;
RA El-Sayed N.M., Myler P.J., Blandin G., Berriman M., Crabtree J.,
RA Aggarwal G., Caler E., Renauld H., Worthey E.A., Hertz-Fowler C.,
RA Ghedin E., Peacock C., Bartholomeu D.C., Haas B.J., Tran A.N.,
RA Wortman J.R., Alsmark U.C., Angiuoli S., Anupama A., Badger J.,
RA Bringaud F., Cadag E., Carlton J.M., Cerqueira G.C., Creasy T.,
RA Delcher A.L., Djikeng A., Embley T.M., Hauser C., Ivens A.C.,
RA Kummerfeld S.K., Pereira-Leal J.B., Nilsson D., Peterson J., Salzberg S.L.,
RA Shallom J., Silva J.C., Sundaram J., Westenberger S., White O.,
RA Melville S.E., Donelson J.E., Andersson B., Stuart K.D., Hall N.;
RT "Comparative genomics of trypanosomatid parasitic protozoa.";
RL Science 309:404-409(2005).
RN [2] {ECO:0000312|Proteomes:UP000008524}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [3] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE, AND MOTIF.
RC STRAIN=427 {ECO:0000303|PubMed:31743541};
RX PubMed=31743541; DOI=10.1111/mmi.14429;
RA Liu B., Kamanyi Marucha K., Clayton C.;
RT "The zinc finger proteins ZC3H20 and ZC3H21 stabilise mRNAs encoding
RT membrane proteins and mitochondrial proteins in insect-form Trypanosoma
RT brucei.";
RL Mol. Microbiol. 113:430-451(2020).
CC -!- FUNCTION: RNA-binding protein involved in regulation of mRNA stability
CC (PubMed:31743541). Promotes mRNA stabilization by recruiting MKT1 and
CC PBP1 (By similarity). Stabilizes transcripts encoding mitochondrial
CC proteins (PubMed:31743541). {ECO:0000250|UniProtKB:Q57W26,
CC ECO:0000269|PubMed:31743541}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the procyclic form.
CC {ECO:0000269|PubMed:31743541}.
CC -!- INDUCTION: Induced during the differentiation of the bloodstream form
CC to the procyclic form. {ECO:0000269|PubMed:31743541}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in procyclic form does
CC not affect growth rate (PubMed:31743541). Simultaneous knockdown of
CC ZC3H20 and ZC3H21 in the procyclic form results in a severe population
CC growth defect and a shift towards the bloodstream form transcriptome
CC (PubMed:31743541). Simultaneous knockdown of ZC3H20 and ZC3H21 in the
CC bloodstream form is lethal (PubMed:31743541).
CC {ECO:0000269|PubMed:31743541}.
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DR EMBL; AC159408; AAX69418.1; -; Genomic_DNA.
DR EMBL; CP000070; AAZ12331.1; -; Genomic_DNA.
DR RefSeq; XP_845890.1; XM_840797.1.
DR AlphaFoldDB; Q57Y77; -.
DR PaxDb; Q57Y77; -.
DR GeneID; 3658479; -.
DR KEGG; tbr:Tb927.7.2670; -.
DR VEuPathDB; TriTrypDB:Tb927.7.2670; -.
DR eggNOG; ENOG502RZ2R; Eukaryota.
DR InParanoid; Q57Y77; -.
DR Proteomes; UP000008524; Chromosome 7.
DR GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0048255; P:mRNA stabilization; IMP:UniProtKB.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IDA:GeneDB.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR InterPro; IPR000571; Znf_CCCH.
DR PROSITE; PS50103; ZF_C3H1; 2.
PE 2: Evidence at transcript level;
KW Metal-binding; Reference proteome; Repeat; RNA-binding;
KW Translation regulation; Zinc; Zinc-finger.
FT CHAIN 1..461
FT /note="RNA-binding protein ZCH321"
FT /id="PRO_0000451931"
FT ZN_FING 63..85
FT /note="C3H1-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 181..208
FT /note="C3H1-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 224..243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 446..451
FT /note="MKT1-binding motif"
FT /evidence="ECO:0000305|PubMed:31743541"
SQ SEQUENCE 461 AA; 49687 MW; 0B9D66318898C36E CRC64;
MSHPQSHRYF SVNAPAGGER HLALDEKGVL CLTVVDPATR KLLIPLSCVY PTQAQQRTTI
PSLCQLFLNG RCRQGTQCHQ VHAALDVVAA LRSQVDYLPI CCALHGDRDY VNALDNRSWM
SRVVVHVPDA TYGGGYIPLA RFSYTTPISR ILREVNARLE SGVSVAAVDG GGHPKRMVLN
ACDFKICGLH TLDRCRYAEE CIFLHICKEI VVDVNNGSGD YSLTSQARDG GEPGPRGAKK
GSVFVSVSRQ QRVRGRPLSY PTVEYQTPFP LRSYEGSMSY NAVSDINSDC SWVSGRYPTG
AYMESRMDAA PLPSLAPRFV QGHPGVPRCY SSTTTSTTTT AAAREDAAMY GAGGCWDRWS
SPESGCAPCA FVGSCSDCHY ADNNNYSGCN GYAYGSRNGY GSDGPNCYFC PGDECSSTHL
CPNGTSSSVP QPVESVSSAS VSARAWQHNP YGVTPTGGIS D
