ZC3HA_HUMAN
ID ZC3HA_HUMAN Reviewed; 434 AA.
AC Q96K80;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Zinc finger CCCH domain-containing protein 10;
GN Name=ZC3H10; Synonyms=ZC3HDC10;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [4]
RP FUNCTION, MIRNA-BINDING, SUBCELLULAR LOCATION, AND MUTAGENESIS OF
RP 50-CYS--CYS-56; 87-CYS--CYS-92 AND 148-CYS--CYS-154.
RX PubMed=28431233; DOI=10.1016/j.molcel.2017.03.014;
RA Treiber T., Treiber N., Plessmann U., Harlander S., Daiss J.L., Eichner N.,
RA Lehmann G., Schall K., Urlaub H., Meister G.;
RT "A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.";
RL Mol. Cell 66:270-284(2017).
CC -!- FUNCTION: Specific regulator of miRNA biogenesis. Binds, via the C3H1-
CC type zinc finger domains, to the binding motif 5'-GCAGCGC-3' on
CC microRNA pri-MIR143 and negatively regulates the processing to mature
CC microRNA. {ECO:0000269|PubMed:28431233}.
CC -!- INTERACTION:
CC Q96K80; P19801: AOC1; NbExp=3; IntAct=EBI-742550, EBI-12826295;
CC Q96K80; Q92624: APPBP2; NbExp=3; IntAct=EBI-742550, EBI-743771;
CC Q96K80; Q03989: ARID5A; NbExp=3; IntAct=EBI-742550, EBI-948603;
CC Q96K80; P54253: ATXN1; NbExp=7; IntAct=EBI-742550, EBI-930964;
CC Q96K80; Q8N9W6-4: BOLL; NbExp=3; IntAct=EBI-742550, EBI-11983447;
CC Q96K80; Q5SWW7: C10orf55; NbExp=3; IntAct=EBI-742550, EBI-12809220;
CC Q96K80; Q5BKX5-3: C19orf54; NbExp=3; IntAct=EBI-742550, EBI-11976299;
CC Q96K80; O43186: CRX; NbExp=3; IntAct=EBI-742550, EBI-748171;
CC Q96K80; O75553: DAB1; NbExp=3; IntAct=EBI-742550, EBI-7875264;
CC Q96K80; Q15038: DAZAP2; NbExp=7; IntAct=EBI-742550, EBI-724310;
CC Q96K80; A1KXE4-2: FAM168B; NbExp=3; IntAct=EBI-742550, EBI-12193763;
CC Q96K80; P53539: FOSB; NbExp=3; IntAct=EBI-742550, EBI-2806743;
CC Q96K80; O75593: FOXH1; NbExp=3; IntAct=EBI-742550, EBI-1759806;
CC Q96K80; Q5TA45: INTS11; NbExp=3; IntAct=EBI-742550, EBI-748258;
CC Q96K80; Q96G42: KLHDC7B; NbExp=3; IntAct=EBI-742550, EBI-9478422;
CC Q96K80; Q8IUB9: KRTAP19-1; NbExp=3; IntAct=EBI-742550, EBI-12811111;
CC Q96K80; Q3LI64: KRTAP6-1; NbExp=3; IntAct=EBI-742550, EBI-12111050;
CC Q96K80; Q3LI66: KRTAP6-2; NbExp=3; IntAct=EBI-742550, EBI-11962084;
CC Q96K80; Q8IUC2: KRTAP8-1; NbExp=3; IntAct=EBI-742550, EBI-10261141;
CC Q96K80; Q14847-2: LASP1; NbExp=3; IntAct=EBI-742550, EBI-9088686;
CC Q96K80; P35548: MSX2; NbExp=3; IntAct=EBI-742550, EBI-6447480;
CC Q96K80; P62166: NCS1; NbExp=3; IntAct=EBI-742550, EBI-746987;
CC Q96K80; P23511-2: NFYA; NbExp=3; IntAct=EBI-742550, EBI-11061759;
CC Q96K80; P32243-2: OTX2; NbExp=3; IntAct=EBI-742550, EBI-9087860;
CC Q96K80; Q8TDS5: OXER1; NbExp=3; IntAct=EBI-742550, EBI-12813389;
CC Q96K80; O75928-2: PIAS2; NbExp=3; IntAct=EBI-742550, EBI-348567;
CC Q96K80; Q13492-3: PICALM; NbExp=3; IntAct=EBI-742550, EBI-11031437;
CC Q96K80; O15496: PLA2G10; NbExp=3; IntAct=EBI-742550, EBI-726466;
CC Q96K80; P14859-6: POU2F1; NbExp=3; IntAct=EBI-742550, EBI-11526590;
CC Q96K80; P86479: PRR20C; NbExp=3; IntAct=EBI-742550, EBI-10172814;
CC Q96K80; P86480: PRR20D; NbExp=3; IntAct=EBI-742550, EBI-12754095;
CC Q96K80; Q9H0Z9: RBM38; NbExp=3; IntAct=EBI-742550, EBI-2840723;
CC Q96K80; Q93062: RBPMS; NbExp=4; IntAct=EBI-742550, EBI-740322;
CC Q96K80; Q6ZRY4: RBPMS2; NbExp=3; IntAct=EBI-742550, EBI-11987469;
CC Q96K80; P78317: RNF4; NbExp=3; IntAct=EBI-742550, EBI-2340927;
CC Q96K80; O75886: STAM2; NbExp=3; IntAct=EBI-742550, EBI-373258;
CC Q96K80; Q96A09: TENT5B; NbExp=3; IntAct=EBI-742550, EBI-752030;
CC Q96K80; Q96LM6: TEX37; NbExp=3; IntAct=EBI-742550, EBI-743976;
CC Q96K80; Q7KZS0: UBE2I; NbExp=3; IntAct=EBI-742550, EBI-10180829;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28431233}.
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DR EMBL; AK027357; BAB55059.1; -; mRNA.
DR EMBL; BC018708; AAH18708.1; -; mRNA.
DR CCDS; CCDS8903.1; -.
DR RefSeq; NP_001290053.1; NM_001303124.1.
DR RefSeq; NP_001290054.1; NM_001303125.1.
DR RefSeq; NP_116175.1; NM_032786.2.
DR AlphaFoldDB; Q96K80; -.
DR BioGRID; 124317; 208.
DR IntAct; Q96K80; 44.
DR STRING; 9606.ENSP00000257940; -.
DR GlyGen; Q96K80; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q96K80; -.
DR PhosphoSitePlus; Q96K80; -.
DR BioMuta; ZC3H10; -.
DR DMDM; 74760908; -.
DR EPD; Q96K80; -.
DR jPOST; Q96K80; -.
DR MassIVE; Q96K80; -.
DR MaxQB; Q96K80; -.
DR PaxDb; Q96K80; -.
DR PeptideAtlas; Q96K80; -.
DR PRIDE; Q96K80; -.
DR ProteomicsDB; 77051; -.
DR Antibodypedia; 27922; 127 antibodies from 17 providers.
DR DNASU; 84872; -.
DR Ensembl; ENST00000257940.7; ENSP00000257940.2; ENSG00000135482.7.
DR GeneID; 84872; -.
DR KEGG; hsa:84872; -.
DR MANE-Select; ENST00000257940.7; ENSP00000257940.2; NM_032786.3; NP_116175.1.
DR UCSC; uc001sjp.2; human.
DR CTD; 84872; -.
DR DisGeNET; 84872; -.
DR GeneCards; ZC3H10; -.
DR HGNC; HGNC:25893; ZC3H10.
DR HPA; ENSG00000135482; Low tissue specificity.
DR neXtProt; NX_Q96K80; -.
DR OpenTargets; ENSG00000135482; -.
DR PharmGKB; PA142670534; -.
DR VEuPathDB; HostDB:ENSG00000135482; -.
DR eggNOG; KOG2494; Eukaryota.
DR GeneTree; ENSGT00950000182897; -.
DR HOGENOM; CLU_039343_0_0_1; -.
DR InParanoid; Q96K80; -.
DR OMA; FIFCHDY; -.
DR OrthoDB; 1441991at2759; -.
DR PhylomeDB; Q96K80; -.
DR TreeFam; TF321931; -.
DR PathwayCommons; Q96K80; -.
DR SignaLink; Q96K80; -.
DR BioGRID-ORCS; 84872; 65 hits in 1080 CRISPR screens.
DR GenomeRNAi; 84872; -.
DR Pharos; Q96K80; Tbio.
DR PRO; PR:Q96K80; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q96K80; protein.
DR Bgee; ENSG00000135482; Expressed in sperm and 173 other tissues.
DR ExpressionAtlas; Q96K80; baseline and differential.
DR Genevisible; Q96K80; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035198; F:miRNA binding; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:1903799; P:negative regulation of miRNA maturation; IDA:UniProtKB.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IDA:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IBA:GO_Central.
DR InterPro; IPR000571; Znf_CCCH.
DR Pfam; PF00642; zf-CCCH; 1.
DR SMART; SM00356; ZnF_C3H1; 3.
DR PROSITE; PS50103; ZF_C3H1; 3.
PE 1: Evidence at protein level;
KW Coiled coil; Metal-binding; Methylation; Nucleus; Reference proteome;
KW Repeat; RNA-binding; Zinc; Zinc-finger.
FT CHAIN 1..434
FT /note="Zinc finger CCCH domain-containing protein 10"
FT /id="PRO_0000281145"
FT ZN_FING 36..63
FT /note="C3H1-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 73..99
FT /note="C3H1-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 134..161
FT /note="C3H1-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 196..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 314..362
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 234..280
FT /evidence="ECO:0000255"
FT COMPBIAS 196..216
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..361
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 185
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q8R205"
FT MOD_RES 186
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q8R205"
FT MUTAGEN 50..56
FT /note="CKRGKRC->SKRGKRS: Abolishes binding to pri-MIR143."
FT /evidence="ECO:0000269|PubMed:28431233"
FT MUTAGEN 87..92
FT /note="CSRPNC->SSRPNS: Abolishes binding to pri-MIR143."
FT /evidence="ECO:0000269|PubMed:28431233"
FT MUTAGEN 148..154
FT /note="CQRGAKC->SQRGAKS: Reduces binding to pri-MIR143."
FT /evidence="ECO:0000269|PubMed:28431233"
SQ SEQUENCE 434 AA; 46052 MW; E4B24A526F3A573B CRC64;
MPDRDSYANG TGSSGGGPGG GGSEEASGAG VGSGGASSDA ICRDFLRNVC KRGKRCRYRH
PDMSEVSNLG VSKNEFIFCH DFQNKECSRP NCRFIHGSKE DEDGYKKTGE LPPRLRQKVA
AGLGLSPADL PNGKEEVPIC RDFLKGDCQR GAKCKFRHLQ RDFEFDARGG GGTGGGSTGS
VLPGRRHDLY DIYDLPDRGF EDHEPGPKRR RGGCCPPDGP HFESYEYSLA PPRGVECRLL
EEENAMLRKR VEELKKQVSN LLATNEVLLE QNAQFRNQAK VITLSSTAPA TEQTLAPTVG
TVATFNHGIA QTHTTLSSQA LQPRPVSQQE LVAPAGAPAA PPTNAAPPAA PPPPPPHLTP
EITPLSAALA QTIAQGMAPP PVSMAPVAVS VAPVAPVAVS MAQPLAGITM SHTTTPMVTY
PIASQSMRIT AMPH
