ID   ZCD_CROSA               Reviewed;         369 AA.
AC   Q84K96;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic;
DE            EC=1.13.11.84 {ECO:0000269|PubMed:12509521};
DE   AltName: Full=CsZCD;
DE   AltName: Full=Zeaxanthin 7,8-dioxygenase;
DE   Flags: Precursor;
GN   Name=ZCD;
OS   Crocus sativus (Saffron).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Iridaceae;
OC   Crocoideae; Croceae; Crocus.
OX   NCBI_TaxID=82528;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, INDUCTION, TISSUE
RP   SPECIFICITY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Style;
RX   PubMed=12509521; DOI=10.1105/tpc.006536;
RA   Bouvier F., Suire C., Mutterer J., Camara B.;
RT   "Oxidative remodeling of chromoplast carotenoids: identification of the
RT   carotenoid dioxygenase CsCCD and CsZCD genes involved in Crocus secondary
RT   metabolite biogenesis.";
RL   Plant Cell 15:47-62(2003).
CC   -!- FUNCTION: Cleaves zeaxanthin symmetrically at the 7-8 and 7'-8' double
CC       bonds to produce crocetin dialdehyde and hydroxy-beta-cyclocitral, two
CC       water-soluble precursors sequestred in vacuoles and involved in the
CC       synthesis of saffron pigment and aroma. {ECO:0000269|PubMed:12509521}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-zeaxanthin + 2 O2 = 2 3beta-hydroxy-beta-cyclocitral
CC         + crocetin dialdehyde; Xref=Rhea:RHEA:26397, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:27547, ChEBI:CHEBI:53166, ChEBI:CHEBI:53167;
CC         EC=1.13.11.84; Evidence={ECO:0000269|PubMed:12509521};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Plastid, chromoplast
CC       {ECO:0000269|PubMed:12509521}.
CC   -!- TISSUE SPECIFICITY: In the style branches.
CC       {ECO:0000269|PubMed:12509521}.
CC   -!- INDUCTION: By dehydration. {ECO:0000269|PubMed:12509521}.
CC   -!- SIMILARITY: Belongs to the carotenoid oxygenase family. {ECO:0000305}.
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DR   EMBL; AJ489276; CAD33262.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q84K96; -.
DR   SMR; Q84K96; -.
DR   BioCyc; MetaCyc:MON-12426; -.
DR   BRENDA; 1.13.11.84; 7275.
DR   GO; GO:0009509; C:chromoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR   InterPro; IPR004294; Carotenoid_Oase.
DR   PANTHER; PTHR10543; PTHR10543; 1.
DR   Pfam; PF03055; RPE65; 1.
PE   1: Evidence at protein level;
KW   Chromoplast; Dioxygenase; Iron; Metal-binding; Oxidoreductase; Plastid;
KW   Stress response; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chromoplast"
FT   CHAIN           ?..369
FT                   /note="Zeaxanthin 7,8(7',8')-cleavage dioxygenase,
FT                   chromoplastic"
FT                   /id="PRO_0000282608"
FT   BINDING         62
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         112
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         177
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         356
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   369 AA;  40750 MW;  7ADF336B2AED9CCD CRC64;
     MQVDPTKGIG LANTSLQFSN GRLHALCEYD LPYVVRLSPE DGDISTVGRI ENNVSTKSTT
     AHPKTDPVTG ETFSFSYGPI QPYVTYSRYD CDGKKSGPDV PIFSFKEPSF VHDFAITEHY
     AVFPDIQIVM KPAEIVRGRR MIGPDLEKVP RLGLLPRYAT SDSEMRWFDV PGFNMVHVVN
     AWEEEGGEVV VIVAPNVSPI ENAIDRFDLL HVSVEMARIE LKSGSVSRTL LSAENLDFGV
     IHRGYSGRKS RYAYLGVGDP MPKIRGVVKV DFELAGRGEC VVARREFGVG CFGGEPFFVP
     ASSKKSGGEE DDGYVVSYLH DEGKGESSFV VMDARSPELE ILAEVVLPRR VPYGFHGLFV
     TEAELLSQQ