ZCHC8_DROME
ID ZCHC8_DROME Reviewed; 553 AA.
AC Q2PE14; Q5BHV6; Q9W153;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Zinc finger CCHC domain-containing protein 8 homolog;
GN ORFNames=CG4622;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59; SER-292; SER-347;
RP TYR-356; SER-421 AND SER-423, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Scaffolding subunit of the trimeric nuclear exosome targeting
CC (NEXT) complex, a complex that directs a subset of non-coding short-
CC lived RNAs for exosomal degradation. The RNA exosome is fundamental for
CC the degradation of RNA in eukaryotic nuclei. May be involved in pre-
CC mRNA splicing. {ECO:0000250|UniProtKB:Q6NZY4}.
CC -!- INTERACTION:
CC Q2PE14; Q9VPK4: dRbm7; NbExp=3; IntAct=EBI-123443, EBI-142275;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q6NZY4}. Note=Excluded from nucleolus.
CC {ECO:0000250|UniProtKB:Q6NZY4}.
CC -!- SIMILARITY: Belongs to the ZCCHC8 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF47224.1; -; Genomic_DNA.
DR EMBL; AE013599; ABC66045.2; -; Genomic_DNA.
DR EMBL; AY061482; AAL29030.1; -; mRNA.
DR EMBL; BT021468; AAX33616.1; -; mRNA.
DR EMBL; BT044256; ACH92321.1; -; mRNA.
DR RefSeq; NP_001033971.2; NM_001038882.3.
DR RefSeq; NP_611929.1; NM_138085.4.
DR AlphaFoldDB; Q2PE14; -.
DR SMR; Q2PE14; -.
DR BioGRID; 63491; 7.
DR IntAct; Q2PE14; 6.
DR STRING; 7227.FBpp0289717; -.
DR iPTMnet; Q2PE14; -.
DR PaxDb; Q2PE14; -.
DR DNASU; 37919; -.
DR EnsemblMetazoa; FBtr0072344; FBpp0072251; FBgn0035021.
DR EnsemblMetazoa; FBtr0300490; FBpp0289717; FBgn0035021.
DR GeneID; 37919; -.
DR KEGG; dme:Dmel_CG4622; -.
DR UCSC; CG4622-RA; d. melanogaster.
DR UCSC; CG4622-RB; d. melanogaster.
DR FlyBase; FBgn0035021; CG4622.
DR VEuPathDB; VectorBase:FBgn0035021; -.
DR eggNOG; KOG2673; Eukaryota.
DR GeneTree; ENSGT00390000011475; -.
DR HOGENOM; CLU_611513_0_0_1; -.
DR InParanoid; Q2PE14; -.
DR OMA; TRHAMGY; -.
DR OrthoDB; 1507566at2759; -.
DR PhylomeDB; Q2PE14; -.
DR BioGRID-ORCS; 37919; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37919; -.
DR PRO; PR:Q2PE14; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0035021; Expressed in cleaving embryo and 25 other tissues.
DR Genevisible; Q2PE14; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0034470; P:ncRNA processing; IBA:GO_Central.
DR InterPro; IPR006568; PSP_pro-rich.
DR InterPro; IPR001878; Znf_CCHC.
DR Pfam; PF04046; PSP; 1.
DR Pfam; PF00098; zf-CCHC; 1.
DR SMART; SM00581; PSP; 1.
DR SMART; SM00343; ZnF_C2HC; 1.
DR PROSITE; PS50158; ZF_CCHC; 1.
PE 1: Evidence at protein level;
KW Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Zinc;
KW Zinc-finger.
FT CHAIN 1..553
FT /note="Zinc finger CCHC domain-containing protein 8
FT homolog"
FT /id="PRO_0000355635"
FT ZN_FING 183..200
FT /note="CCHC-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT REGION 388..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 395..411
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..489
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 59
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 292
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 347
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 356
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 421
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 423
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 44
FT /note="I -> V (in Ref. 4; AAX33616)"
FT /evidence="ECO:0000305"
FT CONFLICT 492..553
FT /note="YEGTPLLKFSVYDRLPVGSNFKVGVSDVINFENLPDSTGKYEQMKDLLKNVR
FT EKMVKLQNEN -> AM (in Ref. 4; AAX33616/ACH92321)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 553 AA; 62405 MW; 63259359FFD6B262 CRC64;
MDNSVILIDD SQNSVVIVDD DVEDGELEDD VVFVCPEELT RKEIEPPTDK IEEKHSNTSI
QDTTEEDGDS LVFEVRFNKE AHFTSLQQQV LVALEGAFAD KQIVFRDNPQ ELMISAFERS
RTLPEEEPQD LFLIDTQPAA KLNAANVPSY KRCNADILDE QTEERKKLKA EAVNKCFRPK
AQSSCFNCGD TEHSLRDCTK PRNNSRITRA RKKMTSRTER YHVDTEQRFG HIRPGKISTK
TRHAMGYSRG QLPFIFYRMR VLGYPPAWLE EAKVQSSGIA LFNADGSEVT KSDEEEGESE
TFKYDVNKIV EYPGFNVQPK ANCFDDFKHH NVPPFQESQS KENFIKSLGE NVINGYKRKK
LVDLPAPHDR VPAPSELLTS FDDYDMELEE ETEDPPLPPS VPPPQPPPPE ECEDGELTAR
SPSPSLEDLK AQQEELLQEL DVNASHNTTA NESKSPTDLD DTSETEVGPS AAESGNNEQS
RSAPSTPFKA SYEGTPLLKF SVYDRLPVGS NFKVGVSDVI NFENLPDSTG KYEQMKDLLK
NVREKMVKLQ NEN
