ZCIS_ARATH
ID ZCIS_ARATH Reviewed; 367 AA.
AC Q9SAC0; B3H769; Q8LD73;
DT 16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=15-cis-zeta-carotene isomerase, chloroplastic;
DE EC=5.2.1.12;
DE Flags: Precursor;
GN Name=Z-ISO; OrderedLocusNames=At1g10830; ORFNames=T16B5.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [7]
RP SUBCELLULAR LOCATION, AND INDUCTION BY LIGHT [LARGE SCALE ANALYSIS].
RX PubMed=19224954; DOI=10.1093/pcp/pcp027;
RA Ishikawa M., Fujiwara M., Sonoike K., Sato N.;
RT "Orthogenomics of photosynthetic organisms: bioinformatic and experimental
RT analysis of chloroplast proteins of endosymbiont origin in Arabidopsis and
RT their counterparts in Synechocystis.";
RL Plant Cell Physiol. 50:773-788(2009).
RN [8]
RP FUNCTION, ALTERNATIVE SPLICING, CATALYTIC ACTIVITY, TISSUE SPECIFICITY,
RP INDUCTION BY LIGHT, AND DISRUPTION PHENOTYPE.
RX PubMed=20335404; DOI=10.1104/pp.110.153916;
RA Chen Y., Li F., Wurtzel E.T.;
RT "Isolation and characterization of the Z-ISO gene encoding a missing
RT component of carotenoid biosynthesis in plants.";
RL Plant Physiol. 153:66-79(2010).
CC -!- FUNCTION: Isomerase involved in the biosynthesis of carotenoids.
CC Catalyzes the cis- to trans-conversion of the 15-cis-bond in 9,15,9'-
CC tri-cis-zeta-carotene. {ECO:0000269|PubMed:20335404}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9,9',15-tri-cis-zeta-carotene = 9,9'-di-cis-zeta-carotene;
CC Xref=Rhea:RHEA:30967, ChEBI:CHEBI:48716, ChEBI:CHEBI:48717;
CC EC=5.2.1.12; Evidence={ECO:0000269|PubMed:20335404};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SAC0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SAC0-2; Sequence=VSP_041999, VSP_042000;
CC -!- TISSUE SPECIFICITY: Expressed in leaves and at lower levels in roots.
CC {ECO:0000269|PubMed:20335404}.
CC -!- INDUCTION: Up-regulated by light. {ECO:0000269|PubMed:19224954,
CC ECO:0000269|PubMed:20335404}.
CC -!- DISRUPTION PHENOTYPE: Lacks carotenoids in the dark and exhibits
CC delayed greening when exposed to light. {ECO:0000269|PubMed:20335404}.
CC -!- MISCELLANEOUS: [Isoform 1]: Major isoform in both etiolated and green
CC leaves.
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DR EMBL; AC007354; AAD31330.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28651.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28652.1; -; Genomic_DNA.
DR EMBL; AK221585; BAD95085.1; -; mRNA.
DR EMBL; AF348578; AAK15549.1; -; mRNA.
DR EMBL; AY086168; AAM63372.1; -; mRNA.
DR EMBL; BX816671; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; A86242; A86242.
DR RefSeq; NP_001117264.1; NM_001123792.1. [Q9SAC0-2]
DR RefSeq; NP_563879.1; NM_100959.5. [Q9SAC0-1]
DR AlphaFoldDB; Q9SAC0; -.
DR STRING; 3702.AT1G10830.1; -.
DR PaxDb; Q9SAC0; -.
DR PRIDE; Q9SAC0; -.
DR ProteomicsDB; 242943; -. [Q9SAC0-1]
DR EnsemblPlants; AT1G10830.1; AT1G10830.1; AT1G10830. [Q9SAC0-1]
DR EnsemblPlants; AT1G10830.2; AT1G10830.2; AT1G10830. [Q9SAC0-2]
DR GeneID; 837626; -.
DR Gramene; AT1G10830.1; AT1G10830.1; AT1G10830. [Q9SAC0-1]
DR Gramene; AT1G10830.2; AT1G10830.2; AT1G10830. [Q9SAC0-2]
DR KEGG; ath:AT1G10830; -.
DR Araport; AT1G10830; -.
DR TAIR; locus:2196475; AT1G10830.
DR eggNOG; ENOG502QSJ3; Eukaryota.
DR HOGENOM; CLU_063140_0_0_1; -.
DR InParanoid; Q9SAC0; -.
DR OMA; DVVWIDN; -.
DR OrthoDB; 803758at2759; -.
DR PhylomeDB; Q9SAC0; -.
DR BioCyc; MetaCyc:AT1G10830-MON; -.
DR PRO; PR:Q9SAC0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SAC0; baseline and differential.
DR Genevisible; Q9SAC0; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0090471; F:9,15,9'-tri-cis-zeta-carotene isomerase activity; IDA:TAIR.
DR GO; GO:0016120; P:carotene biosynthetic process; IMP:TAIR.
DR InterPro; IPR009915; NnrU_dom.
DR Pfam; PF07298; NnrU; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Isomerase; Membrane; Plastid;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..58
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 59..367
FT /note="15-cis-zeta-carotene isomerase, chloroplastic"
FT /id="PRO_0000413962"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 257..285
FT /note="MVGQIVWCLAHTLWIGNTVAASASLGLIA -> VLNISLMCLNMKTISFIFV
FT FQKYRKIGFL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_041999"
FT VAR_SEQ 286..367
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_042000"
FT CONFLICT 71
FT /note="S -> Y (in Ref. 5; AAM63372)"
FT /evidence="ECO:0000305"
FT CONFLICT 304
FT /note="E -> K (in Ref. 5; AAM63372)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 367 AA; 40851 MW; 91BA8E41C01D0705 CRC64;
MAVYHLLLSS PPSLLLLPPS PRRPNLTLIR RIPAHPRLGN STSLLSSSSP VIRKILVRST
LREDQPIASD SESSPTLLIG EDSAAFELGK QKLVSWVYFG VVLGVVLFIL NVVWIDNSTG
FGKSFIDAVS NISGSPEVAM LMLILIFAIV HSGLASLRDI GEKLIGERAF RVLFAGISLP
LAMSTIVYFI NHRYDGSQLW QLQGVPGVHE AIWVANFVSF FFLYPSTFNL LEVAAVDKPK
MHLWETGIMR ITRHPQMVGQ IVWCLAHTLW IGNTVAASAS LGLIAHHLFG AWNGDRRLAK
RYGEDFESIK KRTSVIPFAA IFEGRQVLPE DYYKEFVRLP YLAITALTVG AYFAHPLMQG
ASFRLHW
