ID   ZCIS_MAIZE              Reviewed;         366 AA.
AC   B4FHU1;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=15-cis-zeta-carotene isomerase, chloroplastic;
DE            EC=5.2.1.12;
DE   Flags: Precursor;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. B73;
RA   Yu Y., Currie J., Lomeli R., Angelova A., Collura K., Wissotski M.,
RA   Campos D., Kudrna D., Golser W., Ashely E., Haller K., Descour A.,
RA   Fernandes J., Zuccolo A., Soderlund C., Walbot V.;
RT   "Maize full-length cDNA project.";
RL   Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RX   PubMed=17434985; DOI=10.1104/pp.107.098996;
RA   Li F., Murillo C., Wurtzel E.T.;
RT   "Maize Y9 encodes a product essential for 15-cis-zeta-carotene
RT   isomerization.";
RL   Plant Physiol. 144:1181-1189(2007).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, AND TISSUE SPECIFICITY.
RX   PubMed=20335404; DOI=10.1104/pp.110.153916;
RA   Chen Y., Li F., Wurtzel E.T.;
RT   "Isolation and characterization of the Z-ISO gene encoding a missing
RT   component of carotenoid biosynthesis in plants.";
RL   Plant Physiol. 153:66-79(2010).
CC   -!- FUNCTION: Isomerase involved in the biosynthesis of carotenoids.
CC       Catalyzes the cis- to trans-conversion of the 15-cis-bond in 9,15,9'-
CC       tri-cis-zeta-carotene. {ECO:0000269|PubMed:17434985,
CC       ECO:0000269|PubMed:20335404}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=9,9',15-tri-cis-zeta-carotene = 9,9'-di-cis-zeta-carotene;
CC         Xref=Rhea:RHEA:30967, ChEBI:CHEBI:48716, ChEBI:CHEBI:48717;
CC         EC=5.2.1.12; Evidence={ECO:0000269|PubMed:20335404};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in leaves and roots, and at lower levels
CC       in embryos and endosperm. {ECO:0000269|PubMed:20335404}.
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DR   EMBL; BT036679; ACF81684.1; -; mRNA.
DR   RefSeq; NP_001132720.1; NM_001139248.1.
DR   AlphaFoldDB; B4FHU1; -.
DR   STRING; 4577.GRMZM2G011746_P01; -.
DR   PaxDb; B4FHU1; -.
DR   PRIDE; B4FHU1; -.
DR   EnsemblPlants; Zm00001eb408760_T001; Zm00001eb408760_P001; Zm00001eb408760.
DR   GeneID; 100194203; -.
DR   Gramene; Zm00001eb408760_T001; Zm00001eb408760_P001; Zm00001eb408760.
DR   KEGG; zma:100194203; -.
DR   MaizeGDB; 1167525; -.
DR   eggNOG; ENOG502QSJ3; Eukaryota.
DR   HOGENOM; CLU_063140_0_0_1; -.
DR   OMA; DVVWIDN; -.
DR   OrthoDB; 803758at2759; -.
DR   BioCyc; MetaCyc:MON-15661; -.
DR   BRENDA; 5.2.1.12; 6752.
DR   Proteomes; UP000007305; Chromosome 10.
DR   ExpressionAtlas; B4FHU1; baseline and differential.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090471; F:9,15,9'-tri-cis-zeta-carotene isomerase activity; IEA:EnsemblPlants.
DR   GO; GO:0016120; P:carotene biosynthetic process; IEA:EnsemblPlants.
DR   InterPro; IPR009915; NnrU_dom.
DR   Pfam; PF07298; NnrU; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Isomerase; Membrane; Plastid; Reference proteome;
KW   Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..45
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           46..366
FT                   /note="15-cis-zeta-carotene isomerase, chloroplastic"
FT                   /id="PRO_0000413963"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        171..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        338..358
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   366 AA;  40319 MW;  3D4E9FCDEC76419A CRC64;
     MASQLRLHLA ATPPLLPHRR PHLARPLCPT LNPIRAPLPP LSRVLSHARP ARAVGGGIEP
     KEGVVAEGDE SGGGPVLVGE DSAAFELKDQ SVASWAYFAG ILGAVLVALN VLWIDPSTGV
     GTKFLDAVAS VSDSHEVVML LLTIIFAVVH SGMASLRESG EKIVGERVYR VLFAGISLPL
     AVTTIVYFIN HRYDGTQLWQ VQGITGIHEL LWFSSFISFF FLYPSTFNLL EVAAVDKPKL
     HMWETGIMRI TRHPQMVGQV IWCLAHTLWI GNSVAVAASV GLISHHLFGA WNGDRRLLSR
     YGEAFEVLKK RTSVMPFAAI IDGRQKLPKD YHKEFFRLPY VAITMLTLGA YFAHPLMQAS
     SYQLPW