ZCPW2_MOUSE
ID ZCPW2_MOUSE Reviewed; 331 AA.
AC Q08EN7; A0A5F8MP94;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 02-DEC-2020, sequence version 2.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Zinc finger CW-type PWWP domain protein 2 homolog;
GN Name=Zcwpw2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Histone methylation reader which binds to non-methylated
CC (H3K4me0), monomethylated (H3K4me1), dimethylated (H3K4me2) and
CC trimethylated (H3K4me3) 'Lys-4' on histone H3 (By similarity). The
CC order of binding preference is H3K4me3 > H3K4me2 > H3K4me1 > H3K4me0
CC (By similarity). {ECO:0000250|UniProtKB:Q504Y3}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q08EN7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q08EN7-2; Sequence=VSP_060790, VSP_060791;
CC -!- DOMAIN: The CW-TYPE zinc finger mediates its binding to trimethylated
CC histone H3K4me3. {ECO:0000250|UniProtKB:Q504Y3}.
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DR EMBL; AC157475; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC165253; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC104378; AAI04379.1; -; mRNA.
DR AlphaFoldDB; Q08EN7; -.
DR SMR; Q08EN7; -.
DR PaxDb; Q08EN7; -.
DR PRIDE; Q08EN7; -.
DR ProteomicsDB; 302119; -. [Q08EN7-1]
DR Ensembl; ENSMUST00000187803; ENSMUSP00000159064; ENSMUSG00000032443. [Q08EN7-2]
DR Ensembl; ENSMUST00000191100; ENSMUSP00000159110; ENSMUSG00000032443. [Q08EN7-2]
DR Ensembl; ENSMUST00000238919; ENSMUSP00000158867; ENSMUSG00000032443. [Q08EN7-1]
DR MGI; MGI:1921888; Zcwpw2.
DR VEuPathDB; HostDB:ENSMUSG00000032443; -.
DR GeneTree; ENSGT00560000077278; -.
DR InParanoid; Q08EN7; -.
DR OMA; AFVGHYS; -.
DR PhylomeDB; Q08EN7; -.
DR PRO; PR:Q08EN7; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q08EN7; protein.
DR Bgee; ENSMUSG00000032443; Expressed in spermatid and 35 other tissues.
DR ExpressionAtlas; Q08EN7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0035064; F:methylated histone binding; ISS:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR InterPro; IPR000313; PWWP_dom.
DR InterPro; IPR042778; ZCWPW1/ZCWPW2.
DR InterPro; IPR011124; Znf_CW.
DR PANTHER; PTHR15999; PTHR15999; 1.
DR Pfam; PF00855; PWWP; 1.
DR Pfam; PF07496; zf-CW; 1.
DR PROSITE; PS50812; PWWP; 1.
DR PROSITE; PS51050; ZF_CW; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Metal-binding; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..331
FT /note="Zinc finger CW-type PWWP domain protein 2 homolog"
FT /id="PRO_0000415849"
FT DOMAIN 83..147
FT /note="PWWP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00162"
FT ZN_FING 9..64
FT /note="CW-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT REGION 264..295
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..293
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 18
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 23
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 45
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 56
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT VAR_SEQ 96..125
FT /note="SWPGILCPDPFKGKYVTYDQDGNVEKYYVE -> RQQTVRRRRGGIEVPSRK
FT HTNSTGVPQSKD (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_060790"
FT VAR_SEQ 126..331
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_060791"
SQ SEQUENCE 331 AA; 37519 MW; 073465FC27887ABA CRC64;
MGPAPESSEF VHRTWVQCEN ESCLKWRLLS PAAAAAVNPS EPWYCFMNTD PSYSSCSVSE
EDFPEESQFL ENGYKFVYSQ LPLGSLVLVK LRNWPSWPGI LCPDPFKGKY VTYDQDGNVE
KYYVEFLGDP HSTAWMSAAF VGHFSLTLEA ADCTKKKRWY RSALEEAYQL YRCSAEQRLE
VCCLSKPNRA KTDTKAAVVT KKGMQVSKIN TEKKRPRVRK RKRDAALKCS VEIFCSDEAL
SKENMVVSET EGLLKELEKM LQQIQEPTAR EDESQGEQLS QCSPESPTGS PFQSYHEEDY
IVPDGAGLKA GEYIEIITNN LKKIDALISE F
