ZCRB1_MOUSE
ID ZCRB1_MOUSE Reviewed; 217 AA.
AC Q9CZ96; Q3TDL3;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Zinc finger CCHC-type and RNA-binding motif-containing protein 1;
DE AltName: Full=MADP-1;
DE AltName: Full=U11/U12 small nuclear ribonucleoprotein 31 kDa protein;
DE Short=U11/U12 snRNP 31 kDa protein;
GN Name=Zcrb1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=CD-1 X 129/SvJ; TISSUE=Brain;
RA Gao X., Wang H., He Y., Wang Y., Han J.;
RT "Molecular cloning, identification and chromosomal localization of mouse
RT MADP-1 gene encoding a novel RNA binding protein with zinc finger domain.";
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16959469; DOI=10.1016/j.ygeno.2006.07.009;
RA Wang H., Gao M.X., Li L., Wang B., Hori N., Sato K.;
RT "Isolation, expression, and characterization of the human ZCRB1 gene mapped
RT to 12q12.";
RL Genomics 89:59-69(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-210 AND SER-216, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP INTERACTION WITH ZRSR1.
RX PubMed=29617656; DOI=10.1016/j.celrep.2018.03.028;
RA Horiuchi K., Perez-Cerezales S., Papasaikas P., Ramos-Ibeas P.,
RA Lopez-Cardona A.P., Laguna-Barraza R., Fonseca Balvis N., Pericuesta E.,
RA Fernandez-Gonzalez R., Planells B., Viera A., Suja J.A., Ross P.J.,
RA Alen F., Orio L., Rodriguez de Fonseca F., Pintado B., Valcarcel J.,
RA Gutierrez-Adan A.;
RT "Impaired spermatogenesis, muscle, and erythrocyte function in U12 intron
RT splicing-defective zrsr1 mutant mice.";
RL Cell Rep. 23:143-155(2018).
CC -!- SUBUNIT: Component of the U11/U12 snRNPs that are part of the U12-type
CC spliceosome. Interacts with ZRSR1 (PubMed:29617656). {ECO:0000250,
CC ECO:0000269|PubMed:29617656}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CZ96-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CZ96-2; Sequence=VSP_020918;
CC -!- TISSUE SPECIFICITY: Expressed at higher level in heart and testis, and
CC at lower level in cerebellum. Weakly expressed at low level in liver.
CC {ECO:0000269|PubMed:16959469}.
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DR EMBL; AB095989; BAC23140.1; -; mRNA.
DR EMBL; AK012852; BAB28513.1; -; mRNA.
DR EMBL; AK170136; BAE41588.1; -; mRNA.
DR EMBL; BC058368; AAH58368.1; -; mRNA.
DR CCDS; CCDS27766.1; -. [Q9CZ96-1]
DR CCDS; CCDS84190.1; -. [Q9CZ96-2]
DR RefSeq; NP_001334026.1; NM_001347097.1. [Q9CZ96-2]
DR RefSeq; NP_080301.1; NM_026025.2. [Q9CZ96-1]
DR AlphaFoldDB; Q9CZ96; -.
DR SMR; Q9CZ96; -.
DR STRING; 10090.ENSMUSP00000075441; -.
DR iPTMnet; Q9CZ96; -.
DR PhosphoSitePlus; Q9CZ96; -.
DR EPD; Q9CZ96; -.
DR MaxQB; Q9CZ96; -.
DR PaxDb; Q9CZ96; -.
DR PeptideAtlas; Q9CZ96; -.
DR PRIDE; Q9CZ96; -.
DR ProteomicsDB; 302120; -. [Q9CZ96-1]
DR ProteomicsDB; 302121; -. [Q9CZ96-2]
DR Antibodypedia; 25048; 127 antibodies from 25 providers.
DR Ensembl; ENSMUST00000076070; ENSMUSP00000075441; ENSMUSG00000022635. [Q9CZ96-1]
DR Ensembl; ENSMUST00000162160; ENSMUSP00000124549; ENSMUSG00000022635. [Q9CZ96-2]
DR GeneID; 67197; -.
DR KEGG; mmu:67197; -.
DR UCSC; uc007xiu.1; mouse. [Q9CZ96-1]
DR UCSC; uc007xiv.1; mouse. [Q9CZ96-2]
DR CTD; 85437; -.
DR MGI; MGI:1914447; Zcrb1.
DR VEuPathDB; HostDB:ENSMUSG00000022635; -.
DR eggNOG; KOG0118; Eukaryota.
DR GeneTree; ENSGT00730000111061; -.
DR HOGENOM; CLU_059455_1_0_1; -.
DR InParanoid; Q9CZ96; -.
DR OMA; RXVTIMK; -.
DR OrthoDB; 1422822at2759; -.
DR PhylomeDB; Q9CZ96; -.
DR TreeFam; TF106263; -.
DR Reactome; R-MMU-72165; mRNA Splicing - Minor Pathway.
DR BioGRID-ORCS; 67197; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Zcrb1; mouse.
DR PRO; PR:Q9CZ96; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9CZ96; protein.
DR Bgee; ENSMUSG00000022635; Expressed in floor plate of midbrain and 242 other tissues.
DR ExpressionAtlas; Q9CZ96; baseline and differential.
DR Genevisible; Q9CZ96; MM.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005689; C:U12-type spliceosomal complex; ISS:HGNC-UCL.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd12393; RRM_ZCRB1; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003954; RRM_dom_euk.
DR InterPro; IPR044598; ZCRB1.
DR InterPro; IPR034219; ZCRB1_RRM.
DR InterPro; IPR001878; Znf_CCHC.
DR InterPro; IPR036875; Znf_CCHC_sf.
DR PANTHER; PTHR46259; PTHR46259; 1.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF00098; zf-CCHC; 1.
DR SMART; SM00360; RRM; 1.
DR SMART; SM00361; RRM_1; 1.
DR SMART; SM00343; ZnF_C2HC; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR SUPFAM; SSF57756; SSF57756; 1.
DR PROSITE; PS50102; RRM; 1.
DR PROSITE; PS50158; ZF_CCHC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Metal-binding; mRNA processing; mRNA splicing;
KW Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome;
KW Zinc; Zinc-finger.
FT CHAIN 1..217
FT /note="Zinc finger CCHC-type and RNA-binding motif-
FT containing protein 1"
FT /id="PRO_0000252374"
FT DOMAIN 10..88
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT ZN_FING 105..122
FT /note="CCHC-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT REGION 120..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 148..163
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..217
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 155
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 210
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 216
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 174
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_020918"
SQ SEQUENCE 217 AA; 24583 MW; FB528D59C52FF618 CRC64;
MSGGLAPSKS TVYVSNLPFS LTNNDLYRIF SKYGKVVKVT IMKDKDTRKS KGVAFILFLD
KDSALNCTRA INNKQLFGRV IKASIAIDNG RAAEFIRRRN YFDKSKCYEC GESGHLSYAC
PKNMLGEREP PKKKEKKKKR KLPEPEEEIE EVEVSEEEGE DPALDSLSQA IAFQQAKIEE
EQSKWRPNPG GPSTSDDSRR PRIKKSAYFS DEEELSD
