ZD16A_DANRE
ID ZD16A_DANRE Reviewed; 387 AA.
AC B8A4F0; Q7SXG0;
DT 22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Palmitoyltransferase ZDHHC16A {ECO:0000305};
DE EC=2.3.1.225 {ECO:0000250|UniProtKB:Q969W1};
DE AltName: Full=Zinc finger DHHC domain-containing protein 16A {ECO:0000305};
DE Short=DHHC-16A {ECO:0000305};
GN Name=zdhhc16a {ECO:0000312|ZFIN:ZDB-GENE-040426-1621};
GN Synonyms=zdhhc16 {ECO:0000303|PubMed:26663717};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=26663717; DOI=10.1002/dneu.22372;
RA Shi W., Chen X., Wang F., Gao M., Yang Y., Du Z., Wang C., Yao Y., He K.,
RA Hao A.;
RT "ZDHHC16 modulates FGF/ERK dependent proliferation of neural
RT stem/progenitor cells in the zebrafish telencephalon.";
RL Dev. Neurobiol. 76:1014-1028(2016).
CC -!- FUNCTION: Palmitoyl acyltransferase that mediates palmitoylation of
CC proteins and is required during embryonic heart development. Involved
CC in the proliferation of neural stem cells by regulating the FGF/ERK
CC pathway (By similarity). Involved in the proliferation of neural stem
CC cells by regulating the FGF/ERK pathway (PubMed:26663717).
CC {ECO:0000250|UniProtKB:Q969W1, ECO:0000250|UniProtKB:Q9ESG8,
CC ECO:0000269|PubMed:26663717}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225; Evidence={ECO:0000250|UniProtKB:Q969W1};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q969W1}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9ESG8}.
CC -!- TISSUE SPECIFICITY: Expressed in the central nervous system (CNS)
CC (PubMed:26663717). Expressed in the developing forebrain, and
CC especially in the telencephalon (PubMed:26663717).
CC {ECO:0000269|PubMed:26663717}.
CC -!- DEVELOPMENTAL STAGE: Maternally expressed in the at four-cell stage.
CC Highly expressed in the developing anterior neural plate since tailbud
CC stage. During the segmentation period, 24 hour post fertilization
CC (hpf), expression is still enriched in cell clusters at prosencephalon
CC and mesencephalon. {ECO:0000269|PubMed:26663717}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250|UniProtKB:Q8IUH5}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein causes
CC malformation in telencephalon (PubMed:26663717). Impaired neural
CC stem/progenitor cells (NSPCs) proliferation in the telencephalon
CC (PubMed:26663717). {ECO:0000269|PubMed:26663717}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC {ECO:0000305}.
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DR EMBL; BX072537; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC055620; AAH55620.1; -; mRNA.
DR RefSeq; NP_957336.1; NM_201042.1.
DR RefSeq; XP_017214211.1; XM_017358722.1.
DR AlphaFoldDB; B8A4F0; -.
DR STRING; 7955.ENSDARP00000119367; -.
DR PaxDb; B8A4F0; -.
DR Ensembl; ENSDART00000134630; ENSDARP00000119367; ENSDARG00000007808.
DR GeneID; 394017; -.
DR KEGG; dre:394017; -.
DR CTD; 394017; -.
DR ZFIN; ZDB-GENE-040426-1621; zdhhc16a.
DR eggNOG; KOG1313; Eukaryota.
DR GeneTree; ENSGT00940000155032; -.
DR HOGENOM; CLU_054274_0_0_1; -.
DR InParanoid; B8A4F0; -.
DR OMA; VEAVSMC; -.
DR OrthoDB; 1491968at2759; -.
DR PhylomeDB; B8A4F0; -.
DR TreeFam; TF320809; -.
DR PRO; PR:B8A4F0; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 13.
DR Bgee; ENSDARG00000007808; Expressed in early embryo and 25 other tissues.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016409; F:palmitoyltransferase activity; ISS:UniProtKB.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:0021898; P:commitment of multipotent stem cells to neuronal lineage in forebrain; IMP:ZFIN.
DR GO; GO:0001654; P:eye development; ISS:UniProtKB.
DR GO; GO:0021899; P:fibroblast growth factor receptor signaling pathway involved in forebrain neuron fate commitment; IMP:ZFIN.
DR GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR GO; GO:0018345; P:protein palmitoylation; ISS:UniProtKB.
DR GO; GO:0021537; P:telencephalon development; IMP:UniProtKB.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR InterPro; IPR039859; ZDH16.
DR PANTHER; PTHR12246; PTHR12246; 1.
DR Pfam; PF01529; DHHC; 1.
DR PROSITE; PS50216; DHHC; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Endoplasmic reticulum; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..387
FT /note="Palmitoyltransferase ZDHHC16A"
FT /id="PRO_0000442461"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 150..200
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT ACT_SITE 180
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT CONFLICT 41
FT /note="F -> S (in Ref. 2; AAH55620)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="T -> A (in Ref. 2; AAH55620)"
FT /evidence="ECO:0000305"
FT CONFLICT 371
FT /note="D -> G (in Ref. 2; AAH55620)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 387 AA; 44531 MW; 0454F0A56B929858 CRC64;
MHPCSSVLHL LLRCMRGCCR HTRSRVPRRL RRHVSYIRLI FKSLYFNSLT NSDVVTDSIL
EPVFWMVEVV TRWFGMVFVF LVVALTSSVV FIAYFCLLPL VLHTYSPGWM IWHICYGHWN
LVMIVFHYYK ATKTPPGYPP KMKTDVPFVS VCKKCIIPKP ARSHHCGICK TCILKMDHHC
PWLNNCVGHF NHRYFFSFCL FLTLGCMYCS VSGRHLFIDA YNTIDQLKHL EAEKQGVPVT
GIGLLIGIVP SAGVAGKAVQ VAQEVSQPPY TYKDRMFHKS VIYMWVLTST VSVALGALTL
WHALLITRGE TSIERHINGK EAKRLAKRGR VYRNPFSYGK LNNWKVFFGV EKRSHWLTRV
LLPSGHAPYG DGLTWDIYPL KKDMMPV
