ZD18A_DANRE
ID ZD18A_DANRE Reviewed; 467 AA.
AC E7FH11; F1Q6I2; Q08C71;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 3.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Palmitoyltransferase ZDHHC18-A {ECO:0000305};
DE EC=2.3.1.225 {ECO:0000250|UniProtKB:Q9NUE0};
DE AltName: Full=Zinc finger DHHC domain-containing protein 18-A {ECO:0000303|PubMed:27235108, ECO:0000312|ZFIN:ZDB-GENE-060929-424};
GN Name=zdhhc18a {ECO:0000303|PubMed:27235108,
GN ECO:0000312|ZFIN:ZDB-GENE-060929-424};
GN Synonyms=pigv {ECO:0000312|ZFIN:ZDB-GENE-060929-424};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB; TISSUE=Embryo {ECO:0000312|EMBL:AAI24361.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=27235108; DOI=10.1016/j.bbrc.2016.05.074;
RA Du Z., Chen X., Li X., He K., Ji S., Shi W., Hao A.;
RT "Protein palmitoylation activate zygotic gene expression during the
RT maternal-to-zygotic transition.";
RL Biochem. Biophys. Res. Commun. 475:194-201(2016).
CC -!- FUNCTION: Palmitoyltransferase that could catalyze the addition of
CC palmitate onto various protein substrates.
CC {ECO:0000250|UniProtKB:Q9NUE0}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225; Evidence={ECO:0000250|UniProtKB:Q9NUE0};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36684;
CC Evidence={ECO:0000250|UniProtKB:Q9NUE0};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q9NUE0}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: Probably maternally supplied, the zygotic
CC expression becomes significative at 4 hpf.
CC {ECO:0000269|PubMed:27235108}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250|UniProtKB:Q8IUH5}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC ERF2/ZDHHC9 subfamily. {ECO:0000305}.
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DR EMBL; BX649265; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC124360; AAI24361.1; -; mRNA.
DR RefSeq; NP_001071031.1; NM_001077563.1.
DR AlphaFoldDB; E7FH11; -.
DR SMR; E7FH11; -.
DR Ensembl; ENSDART00000101885; ENSDARP00000092660; ENSDARG00000069807.
DR Ensembl; ENSDART00000130540; ENSDARP00000112051; ENSDARG00000069807.
DR GeneID; 564062; -.
DR KEGG; dre:564062; -.
DR CTD; 564062; -.
DR ZFIN; ZDB-GENE-060929-424; zdhhc18a.
DR GeneTree; ENSGT00940000156585; -.
DR HOGENOM; CLU_018741_0_0_1; -.
DR InParanoid; E7FH11; -.
DR OrthoDB; 1264614at2759; -.
DR TreeFam; TF312923; -.
DR TreeFam; TF314515; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 16.
DR Bgee; ENSDARG00000069807; Expressed in cleaving embryo and 24 other tissues.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016409; F:palmitoyltransferase activity; ISS:UniProtKB.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IBA:GO_Central.
DR GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; IBA:GO_Central.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR Pfam; PF01529; DHHC; 1.
DR PROSITE; PS50216; DHHC; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Golgi apparatus; Lipoprotein; Membrane; Palmitate;
KW Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..467
FT /note="Palmitoyltransferase ZDHHC18-A"
FT /id="PRO_0000451102"
FT TOPO_DOM 1..59
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..88
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..204
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..247
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..467
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 161..211
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT ACT_SITE 191
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT CONFLICT 167
FT /note="K -> R (in Ref. 2; AAI24361)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="D -> V (in Ref. 2; AAI24361)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 467 AA; 51688 MW; 5C69D7ADB3E2E575 CRC64;
MKNCEYQQID PRALRTPSSR TSSTLPCGRK GSQRLRRKWE VFPGKNRFYC DGRIMLARQC
GVLPLTIGLI FITSVLFFTF DCPFLVDHLT VFIPVIGGVL FIFVVISLLQ TSFTDPGILP
RALPDEAADI EKQIDNSGSS TYRPPPRTKE ILINDQVVKL KYCFTCKMFR PPRTSHCSLC
DNCVERFDHH CPWVGNCVGK RNYRFFYAFI VSLSFLTSFI FGCVITHLTL RSQGGNGFIQ
AIQDSPASVV ELVICFFSIW SILGLSGFHT YLVASNLTTN EDIKGSWSSK RGEESGNPYT
YNNIFTNCCV VLCGPMPPSL IDRRGFVPPE DAPQTVTSDA ELPAFMAKND TNMCAQGTKE
LLESMANSTV IQSTCAPGKP KTAPVTQESL LNTVSITVSP PSKPPSNGCS GRQARRPIDV
PCSQRGLKRA ALHTHKPMLR LPSPLYSLSD SPLILSDAPD MGFIPLN
