ZDH12_RAT
ID ZDH12_RAT Reviewed; 267 AA.
AC Q6DGF5; Q2TGJ7;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Palmitoyltransferase ZDHHC12 {ECO:0000305};
DE EC=2.3.1.225 {ECO:0000250|UniProtKB:Q8VC90};
DE AltName: Full=DHHC domain-containing cysteine-rich protein 12 {ECO:0000250|UniProtKB:Q96GR4};
DE Short=DHHC-12 {ECO:0000250|UniProtKB:Q96GR4};
DE AltName: Full=Zinc finger DHHC domain-containing protein 12 {ECO:0000312|RGD:1306593};
GN Name=Zdhhc12 {ECO:0000312|RGD:1306593};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Huang C.-H., Chen Y., Ye T.;
RT "A superfamily of membrane-associated DHHC type zinc finger proteins.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Palmitoyltransferase that could catalyze the addition of
CC palmitate onto various protein substrates. Has a palmitoyltransferase
CC activity toward gephyrin/GPHN, regulating its clustering at synapses
CC and its function in gamma-aminobutyric acid receptor clustering.
CC Thereby, indirectly regulates GABAergic synaptic transmission.
CC {ECO:0000250|UniProtKB:Q8VC90}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225; Evidence={ECO:0000250|UniProtKB:Q8VC90};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36684;
CC Evidence={ECO:0000250|UniProtKB:Q8VC90};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q8VC90}; Multi-pass membrane protein
CC {ECO:0000255}. Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q96GR4}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250|UniProtKB:Q8IUH5}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AY886528; AAX73390.1; -; mRNA.
DR EMBL; BC076393; AAH76393.1; -; mRNA.
DR RefSeq; NP_001013257.1; NM_001013239.1.
DR AlphaFoldDB; Q6DGF5; -.
DR SMR; Q6DGF5; -.
DR STRING; 10116.ENSRNOP00000039802; -.
DR PaxDb; Q6DGF5; -.
DR GeneID; 366014; -.
DR KEGG; rno:366014; -.
DR UCSC; RGD:1306593; rat.
DR CTD; 84885; -.
DR RGD; 1306593; Zdhhc12.
DR VEuPathDB; HostDB:ENSRNOG00000015791; -.
DR eggNOG; KOG1311; Eukaryota.
DR HOGENOM; CLU_031257_2_0_1; -.
DR InParanoid; Q6DGF5; -.
DR OMA; RRCRYCM; -.
DR OrthoDB; 1440636at2759; -.
DR PhylomeDB; Q6DGF5; -.
DR PRO; PR:Q6DGF5; -.
DR Proteomes; UP000002494; Chromosome 3.
DR Bgee; ENSRNOG00000015791; Expressed in ovary and 19 other tissues.
DR Genevisible; Q6DGF5; RN.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:RGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016409; F:palmitoyltransferase activity; ISO:RGD.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; ISS:UniProtKB.
DR GO; GO:0097116; P:gephyrin clustering involved in postsynaptic density assembly; ISS:UniProtKB.
DR GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; ISS:UniProtKB.
DR GO; GO:0032230; P:positive regulation of synaptic transmission, GABAergic; ISS:UniProtKB.
DR GO; GO:0018345; P:protein palmitoylation; ISO:RGD.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR Pfam; PF01529; DHHC; 1.
DR PROSITE; PS50216; DHHC; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Endoplasmic reticulum; Golgi apparatus; Lipoprotein;
KW Membrane; Palmitate; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..267
FT /note="Palmitoyltransferase ZDHHC12"
FT /id="PRO_0000212886"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..43
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..178
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..267
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 97..147
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT ACT_SITE 127
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q8VC90"
SQ SEQUENCE 267 AA; 31029 MW; 7D1435B5FC81A7F7 CRC64;
MALWPLLNSG MLVRTGHTVL TWGITLVLFL HDTELRQWEE QGELFLPLTF LLLVLGSLLL
YLAVSLMDPG YVTAQPQPQE EPKEEQTAMV PQAIPLRRCR YCLVLQPLRA RHCRECRRCV
RRYDHHCPWM ENCVGERNHP LFVAYLALQL VVLLWGLYLA WSGLQFFQPW GLWLRSTGLL
FTTFLLLSFF ALVVSLLLAS HLYLVARNTT TWEFISSHRI AYLRQRTSNP FDRGPTRNLA
HFFCGWPSGP WETLWAEEEE EGSSQVV
