ZDH19_HUMAN
ID ZDH19_HUMAN Reviewed; 309 AA.
AC Q8WVZ1; A0A0B4J1W2; A8MSY6; B3KVI1;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Palmitoyltransferase ZDHHC19;
DE EC=2.3.1.225 {ECO:0000269|PubMed:20074548};
DE AltName: Full=Zinc finger DHHC domain-containing protein 19;
DE Short=DHHC-19;
GN Name=ZDHHC19;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, INTERACTION WITH RRAS, ACTIVE SITE, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF CYS-142.
RX PubMed=20074548; DOI=10.1016/j.bbamem.2010.01.002;
RA Baumgart F., Corral-Escariz M., Perez-Gil J., Rodriguez-Crespo I.;
RT "Palmitoylation of R-Ras by human DHHC19, a palmitoyl transferase with a
RT CaaX box.";
RL Biochim. Biophys. Acta 1798:592-604(2010).
RN [5]
RP FUNCTION (MICROBIAL INFECTION).
RX PubMed=30404808; DOI=10.1128/jvi.01747-18;
RA Zhang N., Zhao H., Zhang L.;
RT "Fatty acid synthase promotes the palmitoylation of Chikungunya virus
RT nsP1.";
RL J. Virol. 0:0-0(2018).
RN [6]
RP RETRACTED PAPER.
RX PubMed=31462771; DOI=10.1038/s41586-019-1511-x;
RA Niu J., Sun Y., Chen B., Zheng B., Jarugumilli G.K., Walker S.R.,
RA Hata A.N., Mino-Kenudson M., Frank D.A., Wu X.;
RT "Fatty acids and cancer-amplified ZDHHC19 promote STAT3 activation through
RT S-palmitoylation.";
RL Nature 573:139-143(2019).
RN [7]
RP RETRACTION NOTICE OF PUBMED:31462771.
RX PubMed=32555452; DOI=10.1038/s41586-020-2414-6;
RA Niu J., Sun Y., Chen B., Zheng B., Jarugumilli G.K., Walker S.R.,
RA Hata A.N., Mino-Kenudson M., Frank D.A., Wu X.;
RL Nature 583:154-154(2020).
CC -!- FUNCTION: Palmitoyltransferase that mediates palmitoylation of RRAS,
CC leading to increased cell viability. {ECO:0000269|PubMed:20074548}.
CC -!- FUNCTION: (Microbial infection) Promotes Chikungunya virus (CHIKV)
CC replication by mediating viral nsp1 palmitoylation.
CC {ECO:0000269|PubMed:30404808}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225; Evidence={ECO:0000269|PubMed:20074548};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36684;
CC Evidence={ECO:0000269|PubMed:20074548};
CC -!- INTERACTION:
CC Q8WVZ1-3; P42858: HTT; NbExp=3; IntAct=EBI-25961277, EBI-466029;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:20074548, ECO:0000305}; Multi-pass membrane protein
CC {ECO:0000255}. Cytoplasm, perinuclear region
CC {ECO:0000269|PubMed:20074548}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8WVZ1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8WVZ1-2; Sequence=VSP_056001;
CC Name=3;
CC IsoId=Q8WVZ1-3; Sequence=VSP_060410, VSP_060411;
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250|UniProtKB:Q8IUH5}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC {ECO:0000305}.
CC -!- CAUTION: Was shown to mediate palmitoylation of STAT3, leading to
CC homodimerization and transcriptional activation of STAT3. However, this
CC study was later retracted. {ECO:0000305|PubMed:31462771,
CC ECO:0000305|PubMed:32555452}.
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DR EMBL; AK122907; BAG53793.1; -; mRNA.
DR EMBL; AC069257; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471191; EAW53668.1; -; Genomic_DNA.
DR EMBL; CH471191; EAW53669.1; -; Genomic_DNA.
DR CCDS; CCDS43190.1; -. [Q8WVZ1-1]
DR RefSeq; NP_001034706.1; NM_001039617.1. [Q8WVZ1-1]
DR AlphaFoldDB; Q8WVZ1; -.
DR SMR; Q8WVZ1; -.
DR BioGRID; 126283; 49.
DR IntAct; Q8WVZ1; 3.
DR STRING; 9606.ENSP00000296326; -.
DR PhosphoSitePlus; Q8WVZ1; -.
DR SwissPalm; Q8WVZ1; -.
DR BioMuta; ZDHHC19; -.
DR DMDM; 218511970; -.
DR MassIVE; Q8WVZ1; -.
DR PaxDb; Q8WVZ1; -.
DR PeptideAtlas; Q8WVZ1; -.
DR PRIDE; Q8WVZ1; -.
DR Antibodypedia; 33933; 60 antibodies from 12 providers.
DR DNASU; 131540; -.
DR Ensembl; ENST00000296326.8; ENSP00000296326.3; ENSG00000163958.14. [Q8WVZ1-1]
DR Ensembl; ENST00000397544.6; ENSP00000380678.2; ENSG00000163958.14. [Q8WVZ1-3]
DR Ensembl; ENST00000438232.5; ENSP00000393710.1; ENSG00000163958.14. [Q8WVZ1-2]
DR GeneID; 131540; -.
DR KEGG; hsa:131540; -.
DR MANE-Select; ENST00000296326.8; ENSP00000296326.3; NM_001039617.2; NP_001034706.1.
DR UCSC; uc003fwc.4; human. [Q8WVZ1-1]
DR CTD; 131540; -.
DR DisGeNET; 131540; -.
DR GeneCards; ZDHHC19; -.
DR HGNC; HGNC:20713; ZDHHC19.
DR HPA; ENSG00000163958; Tissue enriched (testis).
DR MIM; 618671; gene.
DR neXtProt; NX_Q8WVZ1; -.
DR OpenTargets; ENSG00000163958; -.
DR PharmGKB; PA134871748; -.
DR VEuPathDB; HostDB:ENSG00000163958; -.
DR eggNOG; KOG1311; Eukaryota.
DR GeneTree; ENSGT00940000161784; -.
DR HOGENOM; CLU_018741_4_0_1; -.
DR InParanoid; Q8WVZ1; -.
DR OMA; NLHPPMS; -.
DR OrthoDB; 1264614at2759; -.
DR PhylomeDB; Q8WVZ1; -.
DR TreeFam; TF319798; -.
DR PathwayCommons; Q8WVZ1; -.
DR SignaLink; Q8WVZ1; -.
DR BioGRID-ORCS; 131540; 14 hits in 1080 CRISPR screens.
DR GenomeRNAi; 131540; -.
DR Pharos; Q8WVZ1; Tdark.
DR PRO; PR:Q8WVZ1; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q8WVZ1; protein.
DR Bgee; ENSG00000163958; Expressed in right testis and 112 other tissues.
DR Genevisible; Q8WVZ1; HS.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0097356; C:perinucleolar compartment; IDA:UniProtKB.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IDA:UniProtKB.
DR GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; IDA:UniProtKB.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR Pfam; PF01529; DHHC; 1.
DR PROSITE; PS50216; DHHC; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Alternative splicing; Cytoplasm; Golgi apparatus;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..309
FT /note="Palmitoyltransferase ZDHHC19"
FT /id="PRO_0000212904"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 112..162
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT REGION 280..309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 142
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067,
FT ECO:0000269|PubMed:20074548"
FT VAR_SEQ 230..309
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056001"
FT VAR_SEQ 259..282
FT /note="YMAEAVQLQRVVGPDWTSMPNLHP -> AAASWMRLASASCRAKPWAVCFPS
FT (in isoform 3)"
FT /id="VSP_060410"
FT VAR_SEQ 283..309
FT /note="Missing (in isoform 3)"
FT /id="VSP_060411"
FT VARIANT 66
FT /note="G -> A (in dbSNP:rs13315830)"
FT /id="VAR_052979"
FT MUTAGEN 142
FT /note="C->S: Abolishes palmitoyltransferase activity."
FT /evidence="ECO:0000269|PubMed:20074548"
SQ SEQUENCE 309 AA; 34352 MW; 645B007F74692FA1 CRC64;
MTLLTDATPL VKEPHPLPLV PRPWFLPSLF AAFNVVLLVF FSGLFFAFPC RWLAQNGEWA
FPVITGSLFV LTFFSLVSLN FSDPGILHQG SAEQGPLTVH VVWVNHGAFR LQWCPKCCFH
RPPRTYHCPW CNICVEDFDH HCKWVNNCIG HRNFRFFMLL VLSLCLYSGA MLVTCLIFLV
RTTHLPFSTD KAIAIVVAVS AAGLLVPLSL LLLIQALSVS SADRTYKGKC RHLQGYNPFD
QGCASNWYLT ICAPLGPKYM AEAVQLQRVV GPDWTSMPNL HPPMSPSALN PPAPTSGSLQ
SREGTPGAW
