ZDH22_RAT
ID ZDH22_RAT Reviewed; 263 AA.
AC Q2TGI8;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Palmitoyltransferase ZDHHC22 {ECO:0000305};
DE EC=2.3.1.225 {ECO:0000250|UniProtKB:Q8N966};
DE AltName: Full=Zinc finger DHHC domain-containing protein 22 {ECO:0000312|RGD:1308446};
DE Short=DHHC-22;
DE Short=zDHHC22;
GN Name=Zdhhc22 {ECO:0000312|RGD:1308446};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Huang C.-H., Chen Y., Ye T.;
RT "A superfamily of membrane-associated DHHC type zinc finger proteins.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Palmitoyltransferase that could catalyze the addition of
CC palmitate onto various protein substrates and be involved in a variety
CC of cellular processes (By similarity). Catalyzes the palmitoylation of
CC KCNMA1, regulating localization of KCNMA1 to the plasma membrane (By
CC similarity). Might also mediate palmitoylation of CNN3 (By similarity).
CC {ECO:0000250|UniProtKB:A0PK84, ECO:0000250|UniProtKB:Q8N966}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225; Evidence={ECO:0000250|UniProtKB:Q8N966};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36684;
CC Evidence={ECO:0000250|UniProtKB:Q8N966};
CC -!- SUBUNIT: Interacts with CNN3. {ECO:0000250|UniProtKB:A0PK84}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q8N966}; Multi-pass membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q8N966};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250|UniProtKB:Q8IUH5}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AY886537; AAX73399.1; -; mRNA.
DR RefSeq; NP_001034414.1; NM_001039325.1.
DR AlphaFoldDB; Q2TGI8; -.
DR STRING; 10116.ENSRNOP00000054651; -.
DR PaxDb; Q2TGI8; -.
DR Ensembl; ENSRNOT00000015046; ENSRNOP00000054651; ENSRNOG00000011285.
DR GeneID; 299211; -.
DR KEGG; rno:299211; -.
DR UCSC; RGD:1308446; rat.
DR CTD; 283576; -.
DR RGD; 1308446; Zdhhc22.
DR eggNOG; KOG1311; Eukaryota.
DR GeneTree; ENSGT00730000111268; -.
DR HOGENOM; CLU_027721_5_3_1; -.
DR InParanoid; Q2TGI8; -.
DR OMA; HFCFFIP; -.
DR OrthoDB; 1402394at2759; -.
DR PhylomeDB; Q2TGI8; -.
DR TreeFam; TF342115; -.
DR PRO; PR:Q2TGI8; -.
DR Proteomes; UP000002494; Chromosome 6.
DR Bgee; ENSRNOG00000011285; Expressed in frontal cortex and 1 other tissue.
DR Genevisible; Q2TGI8; RN.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:RGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISO:RGD.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IBA:GO_Central.
DR GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; IBA:GO_Central.
DR GO; GO:0072659; P:protein localization to plasma membrane; ISO:RGD.
DR GO; GO:0018345; P:protein palmitoylation; ISO:RGD.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR InterPro; IPR000731; SSD.
DR Pfam; PF01529; DHHC; 1.
DR PROSITE; PS50216; DHHC; 1.
DR PROSITE; PS50156; SSD; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Endoplasmic reticulum; Golgi apparatus; Lipoprotein;
KW Membrane; Palmitate; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..263
FT /note="Palmitoyltransferase ZDHHC22"
FT /id="PRO_0000278773"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..47
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..182
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 204..263
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT DOMAIN 91..131
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT ACT_SITE 111
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
SQ SEQUENCE 263 AA; 29249 MW; C3FDBDC8F3997F8F CRC64;
MLALRLLNVV APAYFLCISL VTFVLQLFLF LPSMREDPTA TPLFSPAVLH GALFLFLSAN
ALGNYILVVQ NSPDDLGACQ GTSSQRPQRP PPSTHFCRVC ARVTLRHDHH CFFTGNCIGS
RNMRNFILFC LYTSLACLYS MVAGVAYISA VLSISFAHPL AFLTLLPTSI SQFFSGAVLG
SDMFVILMLY LWFAVGLACA GFCCHQLLLI LRGQTRYQVR KGVAVRARPW RKNLQEVFGK
RWLLGLLVPM FNVGTESSKQ QDK
