ZDH24_ARATH
ID ZDH24_ARATH Reviewed; 407 AA.
AC Q8VYS8; F4K7H9; F6MDM7; Q9FGA9;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Probable protein S-acyltransferase 9;
DE EC=2.3.1.225;
DE AltName: Full=Probable palmitoyltransferase At5g50020;
DE AltName: Full=Zinc finger DHHC domain-containing protein At5g50020;
GN Name=PAT09; OrderedLocusNames=At5g50020; ORFNames=MPF21.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), ALTERNATIVE SPLICING, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22968831; DOI=10.1104/pp.112.203968;
RA Batistic O.;
RT "Genomics and localization of the Arabidopsis DHHC-cysteine-rich domain S-
RT acyltransferase protein family.";
RL Plant Physiol. 160:1597-1612(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND FUNCTION.
RA Hemsley P.A., Taylor L., Grierson C.S.;
RT "S-acylation: dynamic control of plant development and sigalling by lipid
RT modification of proteins.";
RL (In) Proceedings of the 18th international conference on Arabidopsis
RL research, abstract#139, Beijing (2007).
CC -!- FUNCTION: Palmitoyl acyltransferase. {ECO:0000250, ECO:0000269|Ref.5}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8VYS8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VYS8-2; Sequence=VSP_047438, VSP_047439;
CC Name=3;
CC IsoId=Q8VYS8-3; Sequence=VSP_047439;
CC -!- TISSUE SPECIFICITY: Mainly expressed in seeds.
CC {ECO:0000269|PubMed:22968831}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10288.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; JF792493; AEF58502.1; -; mRNA.
DR EMBL; AB026650; BAB10288.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95886.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95887.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68839.1; -; Genomic_DNA.
DR EMBL; AY070050; AAL49807.1; -; mRNA.
DR EMBL; AY096683; AAM20317.1; -; mRNA.
DR RefSeq; NP_001190503.1; NM_001203574.1. [Q8VYS8-2]
DR RefSeq; NP_001330558.1; NM_001344870.1. [Q8VYS8-3]
DR RefSeq; NP_199813.2; NM_124381.3. [Q8VYS8-1]
DR AlphaFoldDB; Q8VYS8; -.
DR SMR; Q8VYS8; -.
DR BioGRID; 20312; 2.
DR IntAct; Q8VYS8; 2.
DR STRING; 3702.AT5G50020.2; -.
DR iPTMnet; Q8VYS8; -.
DR PaxDb; Q8VYS8; -.
DR PRIDE; Q8VYS8; -.
DR ProteomicsDB; 232339; -. [Q8VYS8-1]
DR EnsemblPlants; AT5G50020.1; AT5G50020.1; AT5G50020. [Q8VYS8-1]
DR EnsemblPlants; AT5G50020.2; AT5G50020.2; AT5G50020. [Q8VYS8-2]
DR EnsemblPlants; AT5G50020.3; AT5G50020.3; AT5G50020. [Q8VYS8-3]
DR GeneID; 835066; -.
DR Gramene; AT5G50020.1; AT5G50020.1; AT5G50020. [Q8VYS8-1]
DR Gramene; AT5G50020.2; AT5G50020.2; AT5G50020. [Q8VYS8-2]
DR Gramene; AT5G50020.3; AT5G50020.3; AT5G50020. [Q8VYS8-3]
DR KEGG; ath:AT5G50020; -.
DR Araport; AT5G50020; -.
DR TAIR; locus:2170046; AT5G50020.
DR eggNOG; KOG1311; Eukaryota.
DR InParanoid; Q8VYS8; -.
DR OrthoDB; 1264614at2759; -.
DR PhylomeDB; Q8VYS8; -.
DR BRENDA; 2.3.1.225; 399.
DR PRO; PR:Q8VYS8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VYS8; baseline and differential.
DR Genevisible; Q8VYS8; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IBA:GO_Central.
DR GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; IBA:GO_Central.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR Pfam; PF01529; DHHC; 1.
DR PROSITE; PS50216; DHHC; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Alternative splicing; Cell membrane; Lipoprotein;
KW Membrane; Palmitate; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..407
FT /note="Probable protein S-acyltransferase 9"
FT /id="PRO_0000315352"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 136..179
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT REGION 300..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 305..358
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 166
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1
FT /note="M -> MNDSWSAGPDRILQFVRECSEECLIRSLILM (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_047438"
FT VAR_SEQ 168
FT /note="W -> WVGQCIGV (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:22968831"
FT /id="VSP_047439"
SQ SEQUENCE 407 AA; 47105 MW; D404CBF9F4B5CBB7 CRC64;
MAGRVFEAWK GSNKFLFGGR LIFGPDAWSI PFTFLLIITP VCFFSVFVAT HLRRELLPNN
AGHVFLVAGV LFTVFVLILL FLTSARDPGI VPRNSHPPEE ELCYDTTVSS DGRQTPTVQI
PRTKEVMVYG VSVRVKYCDT CMLYRPPRCS HCSICNNCVE RFDHHCPWRN YRYFFMFVSS
ATILCIYIFS MSALYIKVLM DNHQGTVWRA MRESPWAVML MIYCFISLWF VGGLTGFHLY
LISTNQTTYE NFRYRSDNRI NVYNRGCSNN FFETFCSKVK PSRNDFRAFI KEEPPRNITL
ATTWERPEEA DEENREERRQ KVEDDLDIDE DVMKLQQRLN DEEGSDTAHH KIDIDQMRIG
SNERAPTIRS EARHGNWGAR SNAQEEDVIA GSSVRESRSY AAAEEGR
