ZDHC4_ARATH
ID ZDHC4_ARATH Reviewed; 411 AA.
AC O80685; F4IJ24;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 3.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Probable protein S-acyltransferase 2;
DE EC=2.3.1.225;
DE AltName: Full=Probable palmitoyltransferase At2g40990;
DE AltName: Full=Zinc finger DHHC domain-containing protein At2g40990;
GN Name=PAT02; OrderedLocusNames=At2g40990; ORFNames=T3K9.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND FUNCTION.
RA Hemsley P.A., Taylor L., Grierson C.S.;
RT "S-acylation: dynamic control of plant development and sigalling by lipid
RT modification of proteins.";
RL (In) Proceedings of the 18th international conference on Arabidopsis
RL research, abstract#139, Beijing (2007).
RN [4]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22968831; DOI=10.1104/pp.112.203968;
RA Batistic O.;
RT "Genomics and localization of the Arabidopsis DHHC-cysteine-rich domain S-
RT acyltransferase protein family.";
RL Plant Physiol. 160:1597-1612(2012).
CC -!- FUNCTION: Palmitoyl acyltransferase. {ECO:0000250, ECO:0000269|Ref.3}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers and pollen.
CC {ECO:0000269|PubMed:22968831}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD12012.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004261; AAD12012.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09910.1; -; Genomic_DNA.
DR PIR; T02120; T02120.
DR RefSeq; NP_181632.5; NM_129664.6.
DR AlphaFoldDB; O80685; -.
DR SMR; O80685; -.
DR STRING; 3702.AT2G40990.1; -.
DR iPTMnet; O80685; -.
DR PaxDb; O80685; -.
DR PRIDE; O80685; -.
DR ProteomicsDB; 242932; -.
DR EnsemblPlants; AT2G40990.1; AT2G40990.1; AT2G40990.
DR GeneID; 818699; -.
DR Gramene; AT2G40990.1; AT2G40990.1; AT2G40990.
DR KEGG; ath:AT2G40990; -.
DR Araport; AT2G40990; -.
DR TAIR; locus:2063172; AT2G40990.
DR eggNOG; KOG1311; Eukaryota.
DR HOGENOM; CLU_018741_7_2_1; -.
DR InParanoid; O80685; -.
DR OMA; MEIGGCK; -.
DR OrthoDB; 1264614at2759; -.
DR BRENDA; 2.3.1.225; 399.
DR PRO; PR:O80685; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80685; baseline and differential.
DR Genevisible; O80685; AT.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IBA:GO_Central.
DR GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; IBA:GO_Central.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR Pfam; PF01529; DHHC; 1.
DR PROSITE; PS50216; DHHC; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Cytoplasmic vesicle; Lipoprotein; Membrane; Palmitate;
KW Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..411
FT /note="Probable protein S-acyltransferase 2"
FT /id="PRO_0000363592"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 160..210
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT ACT_SITE 190
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000250"
FT MOD_RES 405
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9M306"
SQ SEQUENCE 411 AA; 46992 MW; 28A965C7C9CACA79 CRC64;
MGRKKSCHVH NAPSDDDIMF SQDHKPKRIY QLWPGNNRFY CGGRLVFGPD ASSLLLTTAM
IGGPALTFCI RMVFLIGKRY PLFHSLILLG ALLLTVLDFT FLFLTSSRDP GIIPRNKEAP
EAEGLDMITQ SSEWVNNKLG NTKIPRTKDI LVNGYTVKVK FCDTCLLYRP PRASHCSICN
NCVQRFDHHC PWVGQCIALR NYPYFICFIS TSTLLCLYVF VFSWVSMLEV HGKMLLMVIT
NDLVFVVLIL YCFVVVWFVG GLTVFHLYLI CTNQTTYENF RYRYDKKENP YGKGLFKNLY
ELFFARIPPP MINFRDWAPE EPDEEVGSIA SELDRTFGPR GDKYDMEMEI GGCKNSKVGL
QLHTLEYDNN NSSEETVKKK GVSEETAAFY IPGIQEPANI TRNSSIDVRS R
