ZDHC8_ARATH
ID ZDHC8_ARATH Reviewed; 706 AA.
AC Q9LIE4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Probable protein S-acyltransferase 20;
DE EC=2.3.1.225;
DE AltName: Full=Probable palmitoyltransferase At3g22180;
DE AltName: Full=Zinc finger DHHC domain-containing protein At3g22180;
GN Name=PAT20; OrderedLocusNames=At3g22180; ORFNames=MKA23.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND FUNCTION.
RA Hemsley P.A., Taylor L., Grierson C.S.;
RT "S-acylation: dynamic control of plant development and sigalling by lipid
RT modification of proteins.";
RL (In) Proceedings of the 18th international conference on Arabidopsis
RL research, abstract#139, Beijing (2007).
RN [4]
RP SUBCELLULAR LOCATION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22968831; DOI=10.1104/pp.112.203968;
RA Batistic O.;
RT "Genomics and localization of the Arabidopsis DHHC-cysteine-rich domain S-
RT acyltransferase protein family.";
RL Plant Physiol. 160:1597-1612(2012).
CC -!- FUNCTION: Palmitoyl acyltransferase. {ECO:0000250, ECO:0000269|Ref.3}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Cytoplasmic vesicle membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB03066.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP001306; BAB03066.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76598.1; -; Genomic_DNA.
DR RefSeq; NP_188857.1; NM_113115.4.
DR AlphaFoldDB; Q9LIE4; -.
DR STRING; 3702.AT3G22180.1; -.
DR iPTMnet; Q9LIE4; -.
DR PaxDb; Q9LIE4; -.
DR PRIDE; Q9LIE4; -.
DR ProteomicsDB; 232310; -.
DR EnsemblPlants; AT3G22180.1; AT3G22180.1; AT3G22180.
DR GeneID; 821782; -.
DR Gramene; AT3G22180.1; AT3G22180.1; AT3G22180.
DR KEGG; ath:AT3G22180; -.
DR Araport; AT3G22180; -.
DR TAIR; locus:2090404; AT3G22180.
DR eggNOG; KOG1311; Eukaryota.
DR HOGENOM; CLU_020283_2_1_1; -.
DR InParanoid; Q9LIE4; -.
DR OMA; PRIMSIF; -.
DR OrthoDB; 290340at2759; -.
DR PhylomeDB; Q9LIE4; -.
DR BRENDA; 2.3.1.225; 399.
DR PRO; PR:Q9LIE4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LIE4; baseline and differential.
DR Genevisible; Q9LIE4; AT.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IBA:GO_Central.
DR GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; IBA:GO_Central.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR Pfam; PF01529; DHHC; 1.
DR PROSITE; PS50216; DHHC; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cell membrane; Cytoplasmic vesicle; Lipoprotein; Membrane;
KW Palmitate; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..706
FT /note="Probable protein S-acyltransferase 20"
FT /id="PRO_0000315405"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 172..222
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT REGION 470..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 591..621
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 680..706
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 591..620
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 202
FT /note="S-palmitoyl cysteine intermediate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 706 AA; 76874 MW; C4BBD759A9626B67 CRC64;
MVRKHGWQLP AHTLQVIAIT VFCLLVVAFY AFFAPFVGGR IWEYVLIGVY SPVAILVFVL
YVRCTAINPA DPRIMSIFDT GVNGDGMVRG LSRNYDETGS QLQASPSVVS RSSTVAGNSS
VKGSVEDAQR VESVSRRSCY NPLAVFCYVF VVEDCRKKEG PAEEQGNSEE ALFCTLCNCE
VRKFSKHCRS CDKCVDCFDH HCKWLNNCVG RKNYVTFVSL MSASLLWLII EAAVGIAVIV
RVFVNKQTME TEIVNRLGNS FSRAPLAAVV GLCTAVAIFA CFPLGELLFF HMLLIKKGIT
TYEYVVAMRA MSEAPDGASV DEEIQNVLYS PTGSATTGFS GGSSLGLPYR GVWCTPPRVF
DNQDEVIPHL DPCMVPSTVD PDAPGSEKGT KALKRPVKRN AWKLAKLDPN EAARAAARAR
ASSSVLRPID NRHLPDNDLS SIGTVSIISS VSTDANVAAS KEIRNNDLRS SLSRNSFAPS
QGSRDEYDTG SHGMSNLSSP SHVHESVTLA PLPQNPTIVG NRFTATSHHM HSTFDDKVLH
RGNDADPLFL FAPATSHLRD VRKTSVVWDP EAGRYVSAPV TTTSEVRNRL LNPSSQTAST
QNPRPILPAH DSSSGSSALR DPLPLHQAER RLTYTGDSIF YGGPLINIPT RDTPRSGRGL
VRDVQDRLAS TVHRDARIRR DSTSNQLPVF APGGLGANSQ TGSNIK
