ZDHC8_DICDI
ID ZDHC8_DICDI Reviewed; 375 AA.
AC Q54VH7;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Putative ZDHHC-type palmitoyltransferase 8;
DE EC=2.3.1.225;
DE AltName: Full=Zinc finger DHHC domain-containing protein 8;
GN ORFNames=DDB_G0280329;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1] {ECO:0000305, ECO:0000312|EMBL:EAL67391.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4 {ECO:0000312|EMBL:EAL67391.1};
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AX4;
RX PubMed=15010511; DOI=10.1093/nar/gkh262;
RA Urushihara H., Morio T., Saito T., Kohara Y., Koriki E., Ochiai H.,
RA Maeda M., Williams J.G., Takeuchi I., Tanaka Y.;
RT "Analyses of cDNAs from growth and slug stages of Dictyostelium
RT discoideum.";
RL Nucleic Acids Res. 32:1647-1653(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000035; EAL67391.1; -; Genomic_DNA.
DR EMBL; BJ358938; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BJ372294; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; C92145; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_641378.1; XM_636286.1.
DR AlphaFoldDB; Q54VH7; -.
DR PaxDb; Q54VH7; -.
DR EnsemblProtists; EAL67391; EAL67391; DDB_G0280329.
DR GeneID; 8622512; -.
DR KEGG; ddi:DDB_G0280329; -.
DR dictyBase; DDB_G0280329; -.
DR eggNOG; KOG1311; Eukaryota.
DR HOGENOM; CLU_738571_0_0_1; -.
DR InParanoid; Q54VH7; -.
DR OMA; GINNHHY; -.
DR PhylomeDB; Q54VH7; -.
DR PRO; PR:Q54VH7; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IBA:GO_Central.
DR GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; IBA:GO_Central.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR Pfam; PF01529; DHHC; 1.
DR PROSITE; PS50216; DHHC; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Glycoprotein; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..375
FT /note="Putative ZDHHC-type palmitoyltransferase 8"
FT /id="PRO_0000262947"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 176..226
FT /note="DHHC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
FT CARBOHYD 95
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 343
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 90
FT /note="N -> K (in Ref. 2; C92145)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 375 AA; 43871 MW; D52D52A04D1644C5 CRC64;
MIDLFDFVVL SSFIILLPLV TDFLNNTFPN AKIGRLAQEV VGMILVFFIV SLIFAGVSLW
YTHFLPFYYT KSLLISFDTL NLLDLLKFIN TDNNNNSGSS IFNKITFYFH IFFTIQLVVN
LYYYYYQTIT ADNFLPKISK NKQIQLFASE TTTTTTTTTD NINEKKNKLC GLCDQVSDGK
WSTINKPKSH HCRICKRCID SMDHHCPFAA NCIGINNHHY FILFIGYTVM ALIYACYLSF
FPYYHCIVNY KNYVSLSFTN DNDNDNNNNN NFKQLAQSCA KFNKYSFIFL CCCLIVTASF
GILLFQTYLI ITNSKTVQLL SRLKKSKSFL DWFKWLYQNF KQNASINNIY SLFPNFKFYN
LIIPYYKRKI NKLNK
