ID   ZDS_ONCHC               Reviewed;         563 AA.
AC   C3VEQ0;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Zeta-carotene desaturase, chloroplastic/chromoplastic;
DE            Short=OgZDS;
DE            EC=1.3.5.6;
DE   AltName: Full=9,9'-di-cis-zeta-carotene desaturase;
DE   AltName: Full=Carotene 7,8-desaturase;
DE   Flags: Precursor;
GN   Name=ZDS;
OS   Oncidium hybrid cultivar (Orchid).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Epidendroideae; Cymbidieae; Oncidiinae; Oncidium.
OX   NCBI_TaxID=141207;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX   PubMed=20635095; DOI=10.1007/s00425-010-1222-x;
RA   Chiou C.Y., Pan H.A., Chuang Y.N., Yeh K.W.;
RT   "Differential expression of carotenoid-related genes determines diversified
RT   carotenoid coloration in floral tissues of Oncidium cultivars.";
RL   Planta 232:937-948(2010).
CC   -!- FUNCTION: Catalyzes the conversion of zeta-carotene to lycopene via the
CC       intermediary of neurosporene. It carries out two consecutive
CC       desaturations (introduction of double bonds) at positions C-7 and C-7'
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=9,9'-di-cis-zeta-carotene + 2 a quinone = 7,7',9,9'-tetra-cis-
CC         lycopene + 2 a quinol; Xref=Rhea:RHEA:30955, ChEBI:CHEBI:24646,
CC         ChEBI:CHEBI:48716, ChEBI:CHEBI:62466, ChEBI:CHEBI:132124; EC=1.3.5.6;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC       Name=NADP(+); Xref=ChEBI:CHEBI:58349; Evidence={ECO:0000250};
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}. Plastid,
CC       chromoplast {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaves, flower buds and lips.
CC       {ECO:0000269|PubMed:20635095}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during floral development.
CC       {ECO:0000269|PubMed:20635095}.
CC   -!- SIMILARITY: Belongs to the zeta carotene desaturase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FJ859990; ACP27625.1; -; mRNA.
DR   AlphaFoldDB; C3VEQ0; -.
DR   SMR; C3VEQ0; -.
DR   UniPathway; UPA00803; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009509; C:chromoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0052887; F:7,9,9'-tricis-neurosporene:quinone oxidoreductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052886; F:9,9'-dicis-carotene:quinone oxidoreductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016719; F:carotene 7,8-desaturase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR014103; Zeta_caro_desat.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR02732; zeta_caro_desat; 1.
PE   2: Evidence at transcript level;
KW   Carotenoid biosynthesis; Chloroplast; Chromoplast; FAD; Flavoprotein; NAD;
KW   Oxidoreductase; Plastid; Transit peptide.
FT   TRANSIT         1..35
FT                   /note="Chloroplast and chromoplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..563
FT                   /note="Zeta-carotene desaturase,
FT                   chloroplastic/chromoplastic"
FT                   /id="PRO_0000426709"
SQ   SEQUENCE   563 AA;  61929 MW;  10EB55B81FB677C9 CRC64;
     MASAACCSSA YVAATSFGNF KGYRRVGASL DRKVSDMSVN APKGLFPPEP DIYSGPKLRV
     AIIGSGLAGM STAVELLDQG HQVDIYESRQ FIGGKVASFV DKHGNHIEMG LHVFFGCYSN
     LFRLMKKVGA DGNLLVKDHT HTFVNKGGDI GELDFRFPVG APIHGIRAFL ATNQLKPYDK
     ARNALALALS PVVRAIVDPN GALQDIRNLD HVSFSDWFFS KGGTRTSIER VWDPVAYAPG
     FIDCDNISAR CMLTIFALFA TKTEASLLRM LKGSPDVYLS GPIKKYIIDR GGRFHLRWGC
     RQILYDKTDS GDAFVTGLAM SKATEKKIVK ADVYVAACDV PGIKKLIPAQ WREWDMFDNL
     FKLVGVPVIT VQLRYNGWVS ELQDIERSRQ LRQAVGLDNL LYTPDADFSC FSDLALASPE
     DYYKEGQGSL IQAVLTPGDP YMPLPNDEIV KRVQKQVLDL FPSSQGLEIL WSSVVKIGQS
     LYREAPGKDP FRPDQETPVK NFFLAGSYTK QDYIDSMEGA TLSGRRAAAY ICGAGEELLS
     LRKKLAADEA HDLSEASNSL SLV