ZEAX_MAIZE
ID ZEAX_MAIZE Reviewed; 263 AA.
AC P04700; Q9SBC4;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2016, sequence version 2.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=22 kDa alpha-zein 16 {ECO:0000303|PubMed:11691845};
DE AltName: Full=22 kDa alpha-zein protein 21 {ECO:0000312|EMBL:AAD09015.1};
DE AltName: Full=22 kDa zein PZ22.1/22A1 {ECO:0000303|PubMed:6286660};
DE AltName: Full=22kD alpha-zein 1 {ECO:0000303|PubMed:11595803};
DE AltName: Full=Protein FLOURY 2;
DE AltName: Full=Zein-alpha PZ22.1/22A1 {ECO:0000303|PubMed:6286660};
DE Flags: Precursor;
GN Name=AZS22-16 {ECO:0000312|EMBL:AAD09015.1};
GN Synonyms=AZ22Z1 {ECO:0000303|PubMed:11595803}, FL2;
GN ORFNames=ZEAMMB73_085745 {ECO:0000312|EMBL:AFW60837.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=6286660; DOI=10.1016/s0021-9258(18)33973-5;
RA Marks M.D., Larkins B.A.;
RT "Analysis of sequence microheterogeneity among zein messenger RNAs.";
RL J. Biol. Chem. 257:9976-9983(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2999157; DOI=10.1016/s0021-9258(17)36258-0;
RA Marks M.D., Lindell J.S., Larkins B.A.;
RT "Nucleotide sequence analysis of zein mRNAs from maize endosperm.";
RL J. Biol. Chem. 260:16451-16459(1985).
RN [3] {ECO:0000312|EMBL:AAD09015.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11691845; DOI=10.1101/gr.197301;
RA Song R., Llaca V., Linton E., Messing J.;
RT "Sequence, regulation, and evolution of the maize 22-kD alpha zein gene
RT family.";
RL Genome Res. 11:1817-1825(2001).
RN [4] {ECO:0000312|EMBL:AFW60837.1, ECO:0000312|Proteomes:UP000007305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [5] {ECO:0000312|EMBL:AAL16989.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Endosperm;
RX PubMed=11595803; DOI=10.2307/3871509;
RA Woo Y.M., Hu D.W., Larkins B.A., Jung R.;
RT "Genomics analysis of genes expressed in maize endosperm identifies novel
RT seed proteins and clarifies patterns of zein gene expression.";
RL Plant Cell 13:2297-2317(2001).
RN [6] {ECO:0000312|EMBL:ACG24580.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT "Insights into corn genes derived from large-scale cDNA sequencing.";
RL Plant Mol. Biol. 69:179-194(2009).
RN [7]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=2999156; DOI=10.1016/s0021-9258(17)36257-9;
RA Marks M.D., Lindell J.S., Larkins B.A.;
RT "Quantitative analysis of the accumulation of Zein mRNA during maize
RT endosperm development.";
RL J. Biol. Chem. 260:16445-16450(1985).
CC -!- FUNCTION: Zeins are major seed storage proteins.
CC {ECO:0000305|PubMed:2999157}.
CC -!- TISSUE SPECIFICITY: Expressed in developing endosperm.
CC {ECO:0000269|PubMed:2999156}.
CC -!- DEVELOPMENTAL STAGE: Maximum expression between 18 and 28 days after
CC pollination. {ECO:0000269|PubMed:2999156}.
CC -!- MISCELLANEOUS: The alpha zeins of 19 kDa and 22 kDa account for 70% of
CC the total zein fraction. They are encoded by a large multigene family.
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: Structurally, 22K and 19K zeins are composed of nine
CC adjacent, topologically antiparallel helices clustered within a
CC distorted cylinder. {ECO:0000250|UniProtKB:P04698}.
CC -!- MISCELLANEOUS: Not expressed in opaque-2 mutants.
CC {ECO:0000269|PubMed:2999156}.
CC -!- MISCELLANEOUS: The floury-2 (fl2) mutation is associated with the
CC synthesis of a novel 24 kDa alpha-zein storage protein and an altered
CC protein body morphology (AC Q41896). The 24 kDa protein is expressed as
CC a 22 kDa alpha-zein 16 with an uncleaved signal peptide, resulting in
CC an enhanced lysine content of the grain and a soft texture of the
CC endosperm. {ECO:0000250|UniProtKB:Q41896}.
CC -!- SIMILARITY: Belongs to the zein family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; V01478; CAA24725.1; -; mRNA.
DR EMBL; AF090446; AAD09015.1; -; Genomic_DNA.
DR EMBL; CM000780; AFW60837.1; -; Genomic_DNA.
DR EMBL; AF371274; AAL16989.1; -; mRNA.
DR EMBL; EU953295; ACG25413.1; -; mRNA.
DR EMBL; EU952462; ACG24580.1; -; mRNA.
DR PIR; A26564; ZIZM21.
DR RefSeq; NP_001105057.1; NM_001111587.1.
DR RefSeq; NP_001106232.1; NM_001112761.1.
DR AlphaFoldDB; P04700; -.
DR STRING; 4577.GRMZM2G397687_P01; -.
DR PaxDb; P04700; -.
DR EnsemblPlants; Zm00001eb170070_T001; Zm00001eb170070_P001; Zm00001eb170070.
DR GeneID; 541924; -.
DR Gramene; Zm00001eb170070_T001; Zm00001eb170070_P001; Zm00001eb170070.
DR KEGG; zma:541924; -.
DR MaizeGDB; 58096; -.
DR eggNOG; ENOG502R48M; Eukaryota.
DR OMA; LQQFMPV; -.
DR OrthoDB; 1842808at2759; -.
DR Proteomes; UP000007305; Chromosome 4.
DR ExpressionAtlas; P04700; baseline and differential.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR InterPro; IPR002530; Zein.
DR Pfam; PF01559; Zein; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..21
FT CHAIN 22..263
FT /note="22 kDa alpha-zein 16"
FT /id="PRO_0000041626"
FT CONFLICT 104
FT /note="V -> M (in Ref. 1 and 2; CAA24725)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 263 AA; 28452 MW; D40553B96495F0D8 CRC64;
MATKILALLA LLALLVSATN AFIIPQCSLA PSASIPQFLP PVTSMGFEHP AVQAYRLQLA
LAASALQQPI AQLQQQSLAH LTLQTIATQQ QQQQFLPSLS HLAVVNPVTY LQQQLLASNP
LALANVAAYQ QQQQLQQFMP VLSQLAMVNP AVYLQLLSSS PLAVGNAPTY LQQQLLQQIV
PALTQLAVAN PAAYLQQLLP FNQLAVSNSA AYLQQRQQLL NPLAVANPLV ATFLQQQQQL
LPYNQFSLMN PALQQPIVGG AIF
