ID   ZEG1_MAIZE              Reviewed;         308 AA.
AC   C0P381; A0A166VB38;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=50 kDa gamma-zein {ECO:0000305};
DE   Flags: Precursor;
GN   ORFNames=GRMZM2G138689 {ECO:0000312|EMBL:ANA76493.1}, Zm.96787;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. B73;
RX   PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA   Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA   Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J.,
RA   Walbot V., Yu Y.;
RT   "Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.";
RL   PLoS Genet. 5:E1000740-E1000740(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. B73;
RX   PubMed=19965430; DOI=10.1126/science.1178534;
RA   Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA   Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA   Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA   Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA   Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA   Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA   Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA   Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA   Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA   Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA   Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA   Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA   Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA   Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA   Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA   Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA   Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA   Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA   Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA   Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA   SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA   Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA   Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA   Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA   Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA   Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT   "The B73 maize genome: complexity, diversity, and dynamics.";
RL   Science 326:1112-1115(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=27092004; DOI=10.1073/pnas.1601352113;
RA   Liu H., Shi J., Sun C., Gong H., Fan X., Qiu F., Huang X., Feng Q.,
RA   Zheng X., Yuan N., Li C., Zhang Z., Deng Y., Wang J., Pan G., Han B.,
RA   Lai J., Wu Y.;
RT   "Gene duplication confers enhanced expression of 27-kDa gamma-zein for
RT   endosperm modification in quality protein maize.";
RL   Proc. Natl. Acad. Sci. U.S.A. 113:4964-4969(2016).
RN   [4]
RP   INTERACTION WITH OP10.
RX   PubMed=27541862; DOI=10.1371/journal.pgen.1006270;
RA   Yao D., Qi W., Li X., Yang Q., Yan S., Ling H., Wang G., Wang G., Song R.;
RT   "Maize opaque10 encodes a cereal-specific protein that is essential for the
RT   proper distribution of zeins in endosperm protein bodies.";
RL   PLoS Genet. 12:E1006270-E1006270(2016).
CC   -!- FUNCTION: Zeins are major seed storage proteins.
CC       {ECO:0000305|PubMed:27092004}.
CC   -!- SUBUNIT: Interacts with OP10 (via N-terminus).
CC       {ECO:0000269|PubMed:27541862}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Lipid-anchor, GPI-
CC       anchor {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the gliadin/glutenin family. {ECO:0000305}.
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DR   EMBL; BT062750; ACN27447.1; -; mRNA.
DR   EMBL; KU593569; ANA76493.1; -; Genomic_DNA.
DR   EMBL; KU593570; ANA76499.1; -; Genomic_DNA.
DR   RefSeq; NP_001105053.2; NM_001111583.2.
DR   AlphaFoldDB; C0P381; -.
DR   STRING; 4577.GRMZM2G138689_P01; -.
DR   PaxDb; C0P381; -.
DR   PRIDE; C0P381; -.
DR   EnsemblPlants; Zm00001eb313790_T001; Zm00001eb313790_P001; Zm00001eb313790.
DR   GeneID; 541920; -.
DR   Gramene; Zm00001eb313790_T001; Zm00001eb313790_P001; Zm00001eb313790.
DR   KEGG; zma:541920; -.
DR   eggNOG; ENOG502R3UM; Eukaryota.
DR   HOGENOM; CLU_907349_0_0_1; -.
DR   OMA; QCAAIHT; -.
DR   OrthoDB; 1572495at2759; -.
DR   Proteomes; UP000007305; Chromosome 7.
DR   ExpressionAtlas; C0P381; baseline and differential.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   InterPro; IPR001954; Glia_glutenin.
DR   PANTHER; PTHR33454; PTHR33454; 1.
DR   Pfam; PF13016; Gliadin; 1.
DR   PRINTS; PR00208; GLIADGLUTEN.
DR   SUPFAM; SSF47699; SSF47699; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW   Reference proteome; Seed storage protein; Signal; Storage protein.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..286
FT                   /note="50 kDa gamma-zein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5007646413"
FT   PROPEP          287..308
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000438211"
FT   REGION          27..159
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        45..121
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        134..159
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           286
FT                   /note="GPI-anchor amidated cysteine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        91
FT                   /note="Missing (in Ref. 3; ANA76493/ANA76499)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   308 AA;  36005 MW;  7724FDB1E1101F7C CRC64;
     MKLVLVVLAF IALVSSVSCT QTGGCSCGQQ QSHEQQHHPQ QHHPQKQQHQ PPPQHHQQQQ
     HQQQQVHMQP QKHQQQQEVH VQQQQQQPQH QQQQQQQQHQ QQHQCEGQQQ HHQQSQGHVQ
     QHEQSHEQHQ GQSHEQQHQQ QFQGHDKQQQ PQQPQQYQQG QEKSQQQQCH CQEQQQTTRC
     SYNYYSSSSN LKNCHEFLRQ QCSPLVMPFL QSRLIQPSSC QVLQQQCCHD LRQIEPQYIH
     QAIYNMVQSI IQEEQQQQPC ELCGSQQATQ SAVAILTAAQ YLPSMCGLYH SYYQNNPCSS
     NDISGVCN