ID   ZEP_ONCHC               Reviewed;         661 AA.
AC   C3VEQ2;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Zeaxanthin epoxidase, chloroplastic;
DE            Short=OgZEP;
DE            EC=1.14.15.21;
DE   Flags: Precursor;
GN   Name=ZEP;
OS   Oncidium hybrid cultivar (Orchid).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Epidendroideae; Cymbidieae; Oncidiinae; Oncidium.
OX   NCBI_TaxID=141207;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX   PubMed=20635095; DOI=10.1007/s00425-010-1222-x;
RA   Chiou C.Y., Pan H.A., Chuang Y.N., Yeh K.W.;
RT   "Differential expression of carotenoid-related genes determines diversified
RT   carotenoid coloration in floral tissues of Oncidium cultivars.";
RL   Planta 232:937-948(2010).
CC   -!- FUNCTION: Zeaxanthin epoxidase that plays an important role in the
CC       xanthophyll cycle and abscisic acid (ABA) biosynthesis. Converts
CC       zeaxanthin into antheraxanthin and subsequently violaxanthin.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-zeaxanthin + 4 H(+) + 2 O2 + 4 reduced [2Fe-2S]-
CC         [ferredoxin] = all-trans-violaxanthin + 2 H2O + 4 oxidized [2Fe-2S]-
CC         [ferredoxin]; Xref=Rhea:RHEA:32443, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:27547, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738,
CC         ChEBI:CHEBI:35288; EC=1.14.15.21;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC   -!- PATHWAY: Plant hormone biosynthesis; abscisate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in flower buds, lips and leaves. Detected
CC       in roots. {ECO:0000269|PubMed:20635095}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during floral development.
CC       {ECO:0000269|PubMed:20635095}.
CC   -!- MISCELLANEOUS: High expression of beta-hydroxylase (BHY) and zeaxanthin
CC       epoxidase (ZEP) results in the accumulation of violaxanthin, 9-cis-
CC       violaxanthin and neoxanthin in the yellow cultivar Gower Ramsey, while
CC       the down-regulation of BHY and ZEP results in the accumulation of beta-
CC       carotene and orange coloration in floral tissues of the cultivar
CC       Sunkist.
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DR   EMBL; FJ859993; ACP27627.1; -; mRNA.
DR   AlphaFoldDB; C3VEQ2; -.
DR   SMR; C3VEQ2; -.
DR   UniPathway; UPA00090; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0052662; F:zeaxanthin epoxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009688; P:abscisic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00060; FHA; 1.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR000253; FHA_dom.
DR   InterPro; IPR008984; SMAD_FHA_dom_sf.
DR   InterPro; IPR017079; Zeaxanthin_epoxidase.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   Pfam; PF00498; FHA; 1.
DR   PIRSF; PIRSF036989; Zeaxanthin_epoxidase; 1.
DR   SUPFAM; SSF49879; SSF49879; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   2: Evidence at transcript level;
KW   Abscisic acid biosynthesis; Chloroplast; FAD; Flavoprotein; Oxidoreductase;
KW   Plastid; Transit peptide.
FT   TRANSIT         1..59
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           60..661
FT                   /note="Zeaxanthin epoxidase, chloroplastic"
FT                   /id="PRO_0000426713"
FT   DOMAIN          555..609
FT                   /note="FHA"
FT                   /evidence="ECO:0000250"
FT   BINDING         81..109
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   BINDING         359..372
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   661 AA;  73797 MW;  C840C5A65EFF5FB7 CRC64;
     MLLFRATLLP SPPFFHKTYF SHLSPVIFSD DPLPVSLQRN RVSGCRKQKW RQIRTLALQS
     DTLSEVRSAP PELNVERNKF RILIAGGGIG GLVLALAAKK KGFDALVFEK DMSAIRGEGQ
     YRGPIQIQSN ALAALEAIDS QVAEEVMGTG CITGDRINGL VDGVSGTWYI KFDTFTPAAE
     RGLPVTRVIS RMSLQQILAR AVGDDAILSD SKIVDFVDYG NKVAVILENG QQYEGDLLVG
     ADGIWSKVRE ILFGYSEPSY SGYTCYTGIA DFVPPDIDTV GYRVFLGHKQ YFVSSDVGAG
     KMQWYAFHNE PPSGSDVPNG KKEILLKIFN GWCDNVIDLI NATEEELILR RDIYDRIPIF
     TWGKGRVTLL GDSVHAMQPN MGQGGCMAIE DSYQLAHELE KARKESIQSR KPMDVKSALK
     RYEKERRLRV AVIYGMARMA AIMASTYRPY LGVGLGPLSF LTKYKIPHPG RTSGRLVIKY
     AMPLMLSWVL GGNSSKLEGR SLTCRLSDKA SDQLRKWFED DDALERALGG EWYLFPLNNG
     DIQPIRLVRD DKRFHIIGSI SHDDSEGISI HLPFPQVHKT HARIACKDNI FYLTDLQSQY
     GTWITDNEGR RYQAPPNVPV RFRSSYSIEF GSDKKVVFKV KVLSTSHKSA VYGGQNLSPV
     V