ZER1_MOUSE
ID ZER1_MOUSE Reviewed; 779 AA.
AC Q80ZJ6; A2BEA2; Q6PGH5; Q8BG38;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Protein zer-1 homolog {ECO:0000305};
DE AltName: Full=Zyg-11 homolog B-like protein;
GN Name=Zer1 {ECO:0000312|MGI:MGI:2442511}; Synonyms=Zyg, Zyg11bl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, Head, Lung, and Olfactory bulb;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Embryonic brain, and Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 504-510, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Serves as substrate adapter subunit in the E3 ubiquitin
CC ligase complex ZYG11B-CUL2-Elongin BC. Acts redudantly with ZYG11B to
CC target substrates bearing N-terminal glycine degrons for proteasomal
CC degradation. Involved in the clearance of proteolytic fragments
CC generated by caspase cleavage during apoptosis since N-terminal glycine
CC degrons are strongly enriched at caspase cleavage sites. Also important
CC in the quality control of protein N-myristoylation in which N-terminal
CC glycine degrons are conditionally exposed after a failure of N-
CC myristoylation. {ECO:0000250|UniProtKB:Q7Z7L7}.
CC -!- SUBUNIT: Interacts with the ELOC-ELOB/Elongin BC complex. Part of an E3
CC ubiquitin ligase complex including ZER1, CUL2 and Elongin BC.
CC {ECO:0000250|UniProtKB:Q7Z7L7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q80ZJ6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80ZJ6-2; Sequence=VSP_014425;
CC Name=3;
CC IsoId=Q80ZJ6-3; Sequence=VSP_014425, VSP_014426, VSP_014427;
CC -!- SIMILARITY: Belongs to the zyg-11 family. {ECO:0000305}.
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DR EMBL; AK029290; BAC26374.1; -; mRNA.
DR EMBL; AK032318; BAC27811.1; -; mRNA.
DR EMBL; AK048853; BAC33474.1; -; mRNA.
DR EMBL; AK087046; BAC39788.1; -; mRNA.
DR EMBL; BX005298; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC048924; AAH48924.1; -; mRNA.
DR EMBL; BC057021; AAH57021.1; -; mRNA.
DR CCDS; CCDS15870.1; -. [Q80ZJ6-1]
DR CCDS; CCDS71017.1; -. [Q80ZJ6-2]
DR RefSeq; NP_001277432.1; NM_001290503.1. [Q80ZJ6-2]
DR RefSeq; NP_848809.2; NM_178694.4. [Q80ZJ6-1]
DR AlphaFoldDB; Q80ZJ6; -.
DR SMR; Q80ZJ6; -.
DR BioGRID; 230663; 19.
DR STRING; 10090.ENSMUSP00000046441; -.
DR iPTMnet; Q80ZJ6; -.
DR PhosphoSitePlus; Q80ZJ6; -.
DR EPD; Q80ZJ6; -.
DR MaxQB; Q80ZJ6; -.
DR PaxDb; Q80ZJ6; -.
DR PRIDE; Q80ZJ6; -.
DR ProteomicsDB; 274978; -. [Q80ZJ6-1]
DR ProteomicsDB; 274979; -. [Q80ZJ6-2]
DR ProteomicsDB; 274980; -. [Q80ZJ6-3]
DR Antibodypedia; 31261; 72 antibodies from 21 providers.
DR DNASU; 227693; -.
DR Ensembl; ENSMUST00000044751; ENSMUSP00000046441; ENSMUSG00000039686. [Q80ZJ6-1]
DR Ensembl; ENSMUST00000113677; ENSMUSP00000109307; ENSMUSG00000039686. [Q80ZJ6-2]
DR GeneID; 227693; -.
DR KEGG; mmu:227693; -.
DR UCSC; uc008jbf.2; mouse. [Q80ZJ6-1]
DR UCSC; uc008jbh.2; mouse. [Q80ZJ6-3]
DR CTD; 10444; -.
DR MGI; MGI:2442511; Zer1.
DR VEuPathDB; HostDB:ENSMUSG00000039686; -.
DR eggNOG; KOG3665; Eukaryota.
DR GeneTree; ENSGT00530000063187; -.
DR HOGENOM; CLU_011533_0_0_1; -.
DR InParanoid; Q80ZJ6; -.
DR OMA; MFDAPAS; -.
DR OrthoDB; 374821at2759; -.
DR PhylomeDB; Q80ZJ6; -.
DR TreeFam; TF313007; -.
DR BioGRID-ORCS; 227693; 5 hits in 74 CRISPR screens.
DR ChiTaRS; Zer1; mouse.
DR PRO; PR:Q80ZJ6; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q80ZJ6; protein.
DR Bgee; ENSMUSG00000039686; Expressed in primary visual cortex and 145 other tissues.
DR ExpressionAtlas; Q80ZJ6; baseline and differential.
DR GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR GO; GO:0006515; P:protein quality control for misfolded or incompletely synthesized proteins; ISS:UniProtKB.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR040368; Zer-1.
DR PANTHER; PTHR12904:SF23; PTHR12904:SF23; 1.
DR SMART; SM00185; ARM; 5.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Direct protein sequencing;
KW Leucine-rich repeat; Reference proteome; Repeat; Ubl conjugation pathway.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q7Z7L7"
FT CHAIN 2..779
FT /note="Protein zer-1 homolog"
FT /id="PRO_0000066599"
FT REPEAT 226..245
FT /note="LRR 1"
FT REPEAT 246..281
FT /note="LRR 2"
FT REPEAT 291..315
FT /note="LRR 3"
FT REPEAT 440..480
FT /note="ARM 1"
FT REPEAT 524..569
FT /note="ARM 2"
FT REPEAT 571..613
FT /note="ARM 3"
FT REPEAT 615..656
FT /note="ARM 4"
FT REPEAT 727..769
FT /note="ARM 5"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q7Z7L7"
FT VAR_SEQ 250..262
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_014425"
FT VAR_SEQ 468..490
FT /note="QRNCCLTLCNFSIPEELEFQYRR -> SPCLLRPACLGPVSLPAPQAPQA
FT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_014426"
FT VAR_SEQ 491..779
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_014427"
SQ SEQUENCE 779 AA; 89076 MW; 825D4B2E2E47A16D CRC64;
MASDTPESLM ALCTDFCLRN LDGTLGYLLD KETLRLHPDI FLPSEICDQL VNEYVELVSA
ACTFEPHETF FSLFSDPRST RLTRIHLRED LVQDQDLEAI RKQDLVELYL TNCEKLSAKS
LQTLRSFRHS LVSLSLSGCA NIFYEEDNPG GCEDECLVNP TCQVLVKDFT FEGFSRLRFL
NLGRMIDGIP VESLLRPLNS LAALDLSGIQ TSDATFLTQW KDSLMSLVLY NMDLSDDHIR
VIVQLHKLRS KILTCGPHLI SSHLDISRDR LSSYYKFKLT RKVLSLLVQK LGNLMSLDIS
GHMILENCSI SKTDEEAGQT STEPSKSSIM PFRALKRPLQ FLGLFETSLC RLTHIPAYKV
SGDKNEEQVL NAIEAYTEHR PEITSRAINL LFDIARIERC NQLLRALKLV ITALKCHKYD
KNIQVTGSAA LFYLTNSEYR SEQSVKLRRQ VIQVVLNGME SYQEVTVQRN CCLTLCNFSI
PEELEFQYRR VNELLLGILS PTRQDESIQR IAVHLCNALV CQVDNDHKEA VGKMGFVVTM
LKLIQKKLLD KTCDQVMEFS WSALWNITDE TPDNCEMFLN FNGMKLFLDC LKEFPEKQEL
HRNMLGLLGN VAEVKELRPQ LMTSQFISVF SNLLESKADG IEVSYNACGV LSHIMFDGPE
AWGVCEPQRA EVEDRMWAAI QSWDINSRRN INYRSFEPIL RLLPQGISPV SQHWATWALY
NLVSVYPDKY CPLLIKEGGM PLLRDLIKMA TARQETKEMA RKVIEHCSNF REENMDTSR
