ZEST_DROME
ID ZEST_DROME Reviewed; 575 AA.
AC P09956; Q24596; Q9V3F1;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Regulatory protein zeste;
GN Name=z; ORFNames=CG7803;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3125410; DOI=10.1007/bf00338402;
RA Mansukhani A., Gunaratne P.H., Sherwood P.W., Sneath B.J., Goldberg M.L.;
RT "Nucleotide sequence and structural analysis of the zeste locus of
RT Drosophila melanogaster.";
RL Mol. Gen. Genet. 211:121-128(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=3582372; DOI=10.1002/j.1460-2075.1987.tb04821.x;
RA Pirrotta V., Manet E., Hardon E., Bickel S.E., Benson M.;
RT "Structure and sequence of the Drosophila zeste gene.";
RL EMBO J. 6:791-799(1987).
RN [3]
RP SEQUENCE REVISION, AND SELF-ASSOCIATION.
RX PubMed=2118108; DOI=10.1002/j.1460-2075.1990.tb07488.x;
RA Bickel S.E., Pirrotta V.;
RT "Self-association of the Drosophila zeste protein is responsible for
RT transvection effects.";
RL EMBO J. 9:2959-2967(1990).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 57-324.
RC STRAIN=ME-K1, ME-K2, ME-LI1, ME-LI2, ME-NJ1, and ME-NJ2;
RX PubMed=8355601; DOI=10.1093/oxfordjournals.molbev.a040044;
RA Hey J., Kliman R.M.;
RT "Population genetics and phylogenetics of DNA sequence variation at
RT multiple loci within the Drosophila melanogaster species complex.";
RL Mol. Biol. Evol. 10:804-822(1993).
RN [8]
RP SELF-ASSOCIATION.
RX PubMed=8491197; DOI=10.1002/j.1460-2075.1993.tb05856.x;
RA Chen J.D., Pirrotta V.;
RT "Multimerization of the Drosophila zeste protein is required for efficient
RT DNA binding.";
RL EMBO J. 12:2075-2083(1993).
CC -!- FUNCTION: Involved in transvection phenomena (= synapsis-dependent gene
CC expression), where the synaptic pairing of chromosomes carrying genes
CC with which zeste interacts influences the expression of these genes.
CC Zeste binds to DNA and stimulates transcription from a nearby promoter.
CC -!- SUBUNIT: Self-associates forming complexes of several hundred monomers.
CC -!- INTERACTION:
CC P09956; Q7KNM2: lwr; NbExp=5; IntAct=EBI-167915, EBI-74966;
CC P09956; Q7KPY3: mor; NbExp=2; IntAct=EBI-167915, EBI-1182199;
CC -!- SUBCELLULAR LOCATION: Nucleus.
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DR EMBL; Y00049; CAA68262.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X06743; CAA29918.1; ALT_SEQ; Genomic_DNA.
DR EMBL; L13043; AAA29026.1; -; Genomic_DNA.
DR EMBL; L13044; AAA29027.1; -; Genomic_DNA.
DR EMBL; L13045; AAA29028.1; -; Genomic_DNA.
DR EMBL; L13046; AAA29029.1; -; Genomic_DNA.
DR EMBL; L13047; AAA29030.1; -; Genomic_DNA.
DR EMBL; L13048; AAA29031.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF45783.1; -; Genomic_DNA.
DR EMBL; AL133505; CAB63525.1; -; Genomic_DNA.
DR PIR; A26639; A26639.
DR PIR; S01272; S01272.
DR RefSeq; NP_001033827.1; NM_001038738.2.
DR RefSeq; NP_525051.1; NM_080312.2.
DR PDB; 5CQQ; X-ray; 3.10 A; A/B=51-130.
DR PDBsum; 5CQQ; -.
DR AlphaFoldDB; P09956; -.
DR SMR; P09956; -.
DR BioGRID; 57766; 44.
DR IntAct; P09956; 18.
DR STRING; 7227.FBpp0070419; -.
DR PaxDb; P09956; -.
DR PRIDE; P09956; -.
DR DNASU; 31230; -.
DR EnsemblMetazoa; FBtr0070435; FBpp0070419; FBgn0004050.
DR GeneID; 31230; -.
DR KEGG; dme:Dmel_CG7803; -.
DR UCSC; CG7803-RA; d. melanogaster.
DR CTD; 22633; -.
DR FlyBase; FBgn0004050; z.
DR VEuPathDB; VectorBase:FBgn0004050; -.
DR eggNOG; ENOG502QR8W; Eukaryota.
DR HOGENOM; CLU_475907_0_0_1; -.
DR InParanoid; P09956; -.
DR OrthoDB; 1065595at2759; -.
DR SignaLink; P09956; -.
DR BioGRID-ORCS; 31230; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31230; -.
DR PRO; PR:P09956; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0004050; Expressed in egg cell and 25 other tissues.
DR ExpressionAtlas; P09956; baseline and differential.
DR Genevisible; P09956; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005705; C:polytene chromosome interband; IDA:FlyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0031507; P:heterochromatin assembly; IDA:FlyBase.
DR GO; GO:0006727; P:ommochrome biosynthetic process; IMP:FlyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:FlyBase.
DR InterPro; IPR028002; Myb_DNA-bind_5.
DR Pfam; PF13873; Myb_DNA-bind_5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..575
FT /note="Regulatory protein zeste"
FT /id="PRO_0000066573"
FT DNA_BIND 48..128
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2..47
FT /note="Hydrophobic"
FT REGION 151..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..52
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 151..166
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 233..234
FT /note="Missing (in strain: ME-K2)"
FT CONFLICT 497
FT /note="S -> A (in Ref. 4 and 6)"
FT /evidence="ECO:0000305"
FT HELIX 58..70
FT /evidence="ECO:0007829|PDB:5CQQ"
FT HELIX 72..75
FT /evidence="ECO:0007829|PDB:5CQQ"
FT HELIX 77..80
FT /evidence="ECO:0007829|PDB:5CQQ"
FT HELIX 89..102
FT /evidence="ECO:0007829|PDB:5CQQ"
FT HELIX 112..124
FT /evidence="ECO:0007829|PDB:5CQQ"
SQ SEQUENCE 575 AA; 61977 MW; 65A8B77C93DF407A CRC64;
MSAQGEGGGA GGSGGGGAGS DGGGNAGQSS TGSGTVAVTN GGNSSAKNQL PLTPRFTAEE
KEVLYTLFHL HEEVIDIKHR KKQRNKYSVR ETWDKIVKDF NSHPHVSAMR NIKQIQKFWL
NSRLRKQYPY RDGSSSNLSS GSAKISSVSV SVASAVPQQQ QQQHHQQHDS VKVEPEYQIS
PDASEHNPQA DTFDEIEMDA NDVSEIDEDP MEQQQQQQQE AQAQAQAQAQ AQAQVQSAAA
EMQKMQQVNA VAAAAAANAT MINTHQINVD QISAEKLTLN DLLHFKTARP REEIILIKHP
EATATQIHTI PTQAQQHPMA TITAGGYNQQ IISEIKPQQI TLAQYQAQQQ QQAQAQAQAQ
AQAQAQAQAQ AQAQAQAQQL AQQQLAAAQH QQLAAAVQVH HQQQQQQQAA VAVQQQQAAA
AAAVKMQLTA ATPTFTFSAL PTVTAATTVP AAVPVPVATA SSGSANSVAV NTSTASSVSI
NNTSLGGGGG NGATNSSATA ADSFEERMNY FKIREAELRC KEQQLATEAK RIELNKAQDE
LKYMKEVHRL RVEELTMKIR ILQKEEEQLR KCSTS
