ZFOI1_MOUSE
ID ZFOI1_MOUSE Reviewed; 680 AA.
AC E9Q8G5;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Zinc finger protein OBI1 {ECO:0000305};
DE AltName: Full=Osteoblast inducer-1 {ECO:0000303|PubMed:30654721};
DE Short=ObI-1 {ECO:0000303|PubMed:30654721};
GN Name=ObI1; Synonyms=A430033K04Rik {ECO:0000312|MGI:MGI:3583896};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP TISSUE SPECIFICITY, FUNCTION, INDUCTION, SUBCELLULAR LOCATION, AND
RP UBIQUITINATION.
RX PubMed=30654721; DOI=10.1089/scd.2018.0152;
RA Querques F., D'Agostino A., Cozzolino C., Cozzuto L., Lombardo B.,
RA Leggiero E., Ruosi C., Pastore L.;
RT "Identification of a Novel Transcription Factor Required for Osteogenic
RT Differentiation of Mesenchymal Stem Cells.";
RL Stem Cells Dev. 28:370-383(2019).
CC -!- FUNCTION: May modulate osteogenic differentiation, at least in part,
CC through the bone morphogenetic protein (BMP) signaling pathway,
CC increasing RUNX2 activation and leading to osteoblast commitment and
CC maturation. {ECO:0000269|PubMed:30654721}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:30654721}.
CC -!- TISSUE SPECIFICITY: Expressed during osteogenic differentiation where
CC levels increase from the first days of differentiation and remain high
CC during the whole process (PubMed:30654721). Highly expressed in lung
CC (PubMed:30654721). {ECO:0000269|PubMed:30654721}.
CC -!- INDUCTION: Up-regulated during osteogenic differentiation.
CC {ECO:0000269|PubMed:30654721}.
CC -!- PTM: Polyubiquitinated, leading to its degradation via the ubiquitin-
CC proteasome pathway. {ECO:0000269|PubMed:30654721}.
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DR EMBL; AC125115; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC242503; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_898846.2; NM_183025.2.
DR AlphaFoldDB; E9Q8G5; -.
DR SMR; E9Q8G5; -.
DR STRING; 10090.ENSMUSP00000067316; -.
DR PaxDb; E9Q8G5; -.
DR PRIDE; E9Q8G5; -.
DR ProteomicsDB; 355325; -.
DR DNASU; 243308; -.
DR Ensembl; ENSMUST00000069862; ENSMUSP00000067316; ENSMUSG00000056014.
DR GeneID; 243308; -.
DR KEGG; mmu:243308; -.
DR UCSC; uc009afu.2; mouse.
DR MGI; MGI:3583896; A430033K04Rik.
DR VEuPathDB; HostDB:ENSMUSG00000056014; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00950000182890; -.
DR HOGENOM; CLU_002678_44_5_1; -.
DR InParanoid; E9Q8G5; -.
DR OMA; FCHESEN; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; E9Q8G5; -.
DR TreeFam; TF339594; -.
DR BioGRID-ORCS; 243308; 0 hits in 65 CRISPR screens.
DR ChiTaRS; A430033K04Rik; mouse.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; E9Q8G5; protein.
DR Bgee; ENSMUSG00000056014; Expressed in animal zygote and 186 other tissues.
DR ExpressionAtlas; E9Q8G5; baseline and differential.
DR Genevisible; E9Q8G5; MM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0045667; P:regulation of osteoblast differentiation; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 6.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 9.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 6.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 8.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 9.
PE 1: Evidence at protein level;
KW Metal-binding; Nucleus; Reference proteome; Repeat; Ubl conjugation; Zinc;
KW Zinc-finger.
FT CHAIN 1..680
FT /note="Zinc finger protein OBI1"
FT /id="PRO_0000454385"
FT DOMAIN 16..87
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 263..280
FT /note="C2H2-type 1; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 455..477
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 483..505
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 511..533
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 539..561
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 567..589
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 595..617
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 623..645
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 651..673
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
SQ SEQUENCE 680 AA; 79457 MW; 4F92FA6E3BF2DFF2 CRC64;
MVRRRQRLPE VDMGLVSFED VAVDFTWQEW QELDAAQRTL YRDVMLENYR SLVWLGHCLA
KPELISKLEE GFEPWGVAEA TEQCLPGVRK WSAPVEKGQQ SQEKYLRQVK IIKKNTPDED
KVEVENTYNV DSNCISNMTL KNEVCSRVFF QELVNPLLDV PLPTEAGERQ STEVPHDLNR
TQEVLSYPKH FTHHSKDQYS QCCFQYFGPD EAFHTKAILT PEMFYVQETS RTCNNYDKSF
DEVTIPAQYM TQLRKQTLGW NICHKIFPNK TELSNHDAMH TGENDDKCDY EKPIINKSLY
LTKHQEAHAG IEPQAHKENI KFFCLDAELQ TVDPELHGEK QVYECKVSGK TFRHQPEHIS
QQRPHACERA CQAKEHGEAG CDEPALTQHQ RLCTEEKACE GKACSKAFHH KSLLPQYQSA
RADEQQSDCK ELMKIYFYVS SPTQHHGPPP PEKPFRCNDC LKTFSHKSQL ERHQRMHTGE
KPHECKECRK AFCHKSHLIR HQGIHAPEKP YECNECKKSF YLRSQLTLHE RTHTGEKPFE
CKECRKAFSR NSHLTQHQKI HTGEKPHKCK ECGNAFARKS HLIQHQKTHT GERPYECKEC
RKAFSRKSQL MQHETTHTGE RAYECKECRK TFYLKAYLTR HQVIHQSEKP FECKKCGKAF
SRKSYLTRHQ KIHKGQTLSG
