ZFP28_MOUSE
ID ZFP28_MOUSE Reviewed; 825 AA.
AC P10078; Q61776; Q8BT11; Q8C836;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 04-AUG-2003, sequence version 3.
DT 03-AUG-2022, entry version 199.
DE RecName: Full=Zinc finger protein 28;
DE Short=Zfp-28;
DE AltName: Full=Protein mKR5;
GN Name=Zfp28; Synonyms=Mkr5, Zfp-28;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 268-825 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=3143103; DOI=10.1093/nar/16.21.9995;
RA Chowdhury K., Rohdewohld H., Gruss P.;
RT "Specific and ubiquitous expression of different Zn finger protein genes in
RT the mouse.";
RL Nucleic Acids Res. 16:9995-10011(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 548-741 (ISOFORM 2), AND VARIANT
RP ILE-658.
RC STRAIN=129/Sv;
RX PubMed=7958967; DOI=10.1016/0378-1119(94)90711-0;
RA Udy G.B.;
RT "Sequence contiguity and allelic structure for the murine zinc finger-
RT encoding gene mKr5.";
RL Gene 148:347-350(1994).
CC -!- FUNCTION: May be involved in transcriptional regulation. May have a
CC role in embryonic development.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P10078-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P10078-2; Sequence=VSP_006882;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in ovary.
CC -!- DEVELOPMENTAL STAGE: Expression decreases in embryo after day 16.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AK028202; BAC25810.1; -; mRNA.
DR EMBL; AK048530; BAC33362.1; -; mRNA.
DR EMBL; BC062116; AAH62116.1; -; mRNA.
DR EMBL; X12594; CAA31107.1; -; mRNA.
DR EMBL; M36516; AAA37120.1; -; mRNA.
DR EMBL; L28802; AAA64428.1; -; Genomic_DNA.
DR CCDS; CCDS39752.1; -. [P10078-1]
DR PIR; I53859; I53859.
DR RefSeq; NP_780456.2; NM_175247.3. [P10078-1]
DR AlphaFoldDB; P10078; -.
DR SMR; P10078; -.
DR BioGRID; 204654; 34.
DR IntAct; P10078; 1.
DR STRING; 10090.ENSMUSP00000079812; -.
DR iPTMnet; P10078; -.
DR PhosphoSitePlus; P10078; -.
DR MaxQB; P10078; -.
DR PaxDb; P10078; -.
DR PRIDE; P10078; -.
DR ProteomicsDB; 275353; -. [P10078-1]
DR ProteomicsDB; 275354; -. [P10078-2]
DR Antibodypedia; 33216; 66 antibodies from 16 providers.
DR DNASU; 22690; -.
DR Ensembl; ENSMUST00000081022; ENSMUSP00000079812; ENSMUSG00000062861. [P10078-1]
DR GeneID; 22690; -.
DR KEGG; mmu:22690; -.
DR UCSC; uc009fbi.1; mouse. [P10078-1]
DR CTD; 140612; -.
DR MGI; MGI:99175; Zfp28.
DR VEuPathDB; HostDB:ENSMUSG00000062861; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162376; -.
DR HOGENOM; CLU_002678_44_5_1; -.
DR InParanoid; P10078; -.
DR OMA; TFRENWD; -.
DR PhylomeDB; P10078; -.
DR TreeFam; TF341817; -.
DR Reactome; R-MMU-212436; Generic Transcription Pathway.
DR BioGRID-ORCS; 22690; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Zfp28; mouse.
DR PRO; PR:P10078; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P10078; protein.
DR Bgee; ENSMUSG00000062861; Expressed in lumbar subsegment of spinal cord and 190 other tissues.
DR ExpressionAtlas; P10078; baseline and differential.
DR Genevisible; P10078; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01352; KRAB; 1.
DR Pfam; PF00096; zf-C2H2; 11.
DR SMART; SM00349; KRAB; 1.
DR SMART; SM00355; ZnF_C2H2; 14.
DR SUPFAM; SSF109640; SSF109640; 1.
DR SUPFAM; SSF57667; SSF57667; 8.
DR PROSITE; PS50805; KRAB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 14.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 14.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..825
FT /note="Zinc finger protein 28"
FT /id="PRO_0000047290"
FT DOMAIN 103..174
FT /note="KRAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT ZN_FING 377..399
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 405..427
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 433..456
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 462..484
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 490..512
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 518..540
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 546..568
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 574..596
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 602..624
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 630..652
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 658..680
FT /note="C2H2-type 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 686..708
FT /note="C2H2-type 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 714..736
FT /note="C2H2-type 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 742..764
FT /note="C2H2-type 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 770..792
FT /note="C2H2-type 15; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 26..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..65
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 600
FT /note="K -> KPFECKVCRKAFRQNIHLASHWRIHTGEKPFECGECGKSFSISSQLA
FT THQRIHTGEK (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_006882"
FT VARIANT 658
FT /note="Y -> I (in strain: 129/Sv; requires 2 nucleotide
FT substitutions)"
FT CONFLICT 263
FT /note="R -> W (in Ref. 1; BAC33362)"
FT /evidence="ECO:0000305"
FT CONFLICT 426
FT /note="C -> Y (in Ref. 3; CAA31107/AAA37120)"
FT /evidence="ECO:0000305"
FT CONFLICT 677
FT /note="R -> Q (in Ref. 3 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 683
FT /note="Q -> L (in Ref. 3 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 704..705
FT /note="HR -> YC (in Ref. 3 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 726
FT /note="R -> W (in Ref. 3 and 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 825 AA; 93309 MW; 19BABAC25F2908E6 CRC64;
MQGMASVVSC EPWALLGRGA LCTKARPGGG PAAGTVVAPG SPDRGRPRSR NSLASQDQQG
AVTSGTAHKA LFSRDTNFLQ EINRKQEAAP TGTRHKAKSQ GLVTFGDVAV VFSQEEWEWL
NSEQRSLYWK VMLDNYRNLA SLGLCASQPD MITSLEQGRD PWMMKRKMRK GQHLDLKAMQ
ETKEFPPKDL SEETLFLAVL RKQLLPHRPK CSMVRAAWEG GAVFTTHRGL KTNSGLARDS
PAQLVSAQRS FCKSVTWENC GDRGSVGQQS VQEAQDLLPR QDSHAERVTG RTWSTKLECS
TFRDQDSECT FERNEQETVT PNRAFSEGRD GMCIESGRWF HLNSSDERSH NCDSGKSFSS
NPVVVKETGI CSGKKLFQCN ECKKTFTQSS SLTVHQRIHT GEKPYKCNQC GKAFSDGSSF
ARHQRCHTGK KPYECPECGK AFIQNTSLVR HWRYYHTGEK PFDCIDCGKA FSDHIGLNQH
RRIHTGEKPY TCEVCHKSFR YGSSLTVHQR IHTGEKPYEC EICRKAFSHH ASLTQHQRVH
SGEKPFKCKE CGKAFRQNIH LASHWRIHTG EKPFECGECG KSFSISSQLA THQRIHTGEK
PYECKVCRKA FTQKAHLAQH QKTHTGEKPY ECKECGKAFS QTTHLIQHQR VHTGEKPYKC
LECGKAFGDN SSCTQHRRLH TGQRPYECVE CGKTFKTKSS LICHRRCHTG EKPYECSACG
KAFSHRQSLS VHQRIHSGKK PYECKECRKT FIQIGHLNQH KRVHTGERTY NYKKGRRAFR
QTAHFAHHQQ IHSGKSPAHH SLPSTSNPVD LFSKFVWNPS SLPSS
