ZFP42_HUMAN
ID ZFP42_HUMAN Reviewed; 310 AA.
AC Q96MM3; D3DP65; Q8WXE2;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Zinc finger protein 42 homolog;
DE Short=Zfp-42;
DE AltName: Full=Reduced expression protein 1;
DE Short=REX-1;
DE Short=hREX-1;
DE AltName: Full=Zinc finger protein 754;
GN Name=ZFP42; Synonyms=REX1, ZNF754;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=12110702; DOI=10.1634/stemcells.20-4-329;
RA Henderson J.K., Draper J.S., Baillie H.S., Fishel S., Thomson J.A.,
RA Moore H., Andrews P.W.;
RT "Preimplantation human embryos and embryonic stem cells show comparable
RT expression of stage-specific embryonic antigens.";
RL Stem Cells 20:329-337(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RA Clapp J., Hewitt J.E.;
RT "A survey of transcripts in the 4q35 FSHD region.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=16424014; DOI=10.1158/0008-5472.can-05-2445;
RA Korkola J.E., Houldsworth J., Chadalavada R.S., Olshen A.B., Dobrzynski D.,
RA Reuter V.E., Bosl G.J., Chaganti R.S.;
RT "Down-regulation of stem cell genes, including those in a 200-kb gene
RT cluster at 12p13.31, is associated with in vivo differentiation of human
RT male germ cell tumors.";
RL Cancer Res. 66:820-827(2006).
RN [7]
RP INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=16344273; DOI=10.1093/carcin/bgi299;
RA Raman J.D., Mongan N.P., Liu L., Tickoo S.K., Nanus D.M., Scherr D.S.,
RA Gudas L.J.;
RT "Decreased expression of the human stem cell marker, Rex-1 (zfp-42), in
RT renal cell carcinoma.";
RL Carcinogenesis 27:499-507(2006).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=16865673; DOI=10.1002/mc.20186;
RA Mongan N.P., Martin K.M., Gudas L.J.;
RT "The putative human stem cell marker, Rex-1 (Zfp42): structural
RT classification and expression in normal human epithelial and carcinoma cell
RT cultures.";
RL Mol. Carcinog. 45:887-900(2006).
CC -!- FUNCTION: Involved in the reprogramming of X-chromosome inactivation
CC during the acquisition of pluripotency. Required for efficient
CC elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34
CC enhancer within the TSIX promoter. Involved in ES cell self-renewal (By
CC similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q96MM3; P50458: LHX2; NbExp=3; IntAct=EBI-12151755, EBI-12179869;
CC Q96MM3; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-12151755, EBI-16439278;
CC Q96MM3; O75928-2: PIAS2; NbExp=3; IntAct=EBI-12151755, EBI-348567;
CC Q96MM3; P78317: RNF4; NbExp=3; IntAct=EBI-12151755, EBI-2340927;
CC Q96MM3; P63165: SUMO1; NbExp=3; IntAct=EBI-12151755, EBI-80140;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in kidney, epidermal keratinocytes,
CC prostate epithelial cells, bronchial and small airway lung epithelial
CC cells (at protein level). Expressed in malignant kidney and several
CC carcinoma cell lines (at protein level). Expressed in embryonic stem
CC cells, kidney, epidermal keratinocytes, prostate epithelial cells,
CC bronchial and small airway lung epithelial cells. Expressed in
CC embryonal carcinomas, seminomas, malignant kidney and several carcinoma
CC cell lines. {ECO:0000269|PubMed:12110702, ECO:0000269|PubMed:16344273,
CC ECO:0000269|PubMed:16424014, ECO:0000269|PubMed:16865673}.
CC -!- INDUCTION: Down-regulated upon retinoic acid addition to F9 stem cells.
CC {ECO:0000269|PubMed:16344273}.
CC -!- PTM: Polyubiquitinated by RNF12, leading to proteasomal degradation.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF450454; AAL47167.1; -; mRNA.
DR EMBL; AY429593; AAR07993.1; -; mRNA.
DR EMBL; AK056719; BAB71264.1; -; mRNA.
DR EMBL; AC108073; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471056; EAX04610.1; -; Genomic_DNA.
DR EMBL; CH471056; EAX04611.1; -; Genomic_DNA.
DR EMBL; CH471056; EAX04612.1; -; Genomic_DNA.
DR CCDS; CCDS3849.1; -.
DR RefSeq; NP_001291287.1; NM_001304358.1.
DR RefSeq; NP_777560.2; NM_174900.4.
DR RefSeq; XP_011529906.1; XM_011531604.2.
DR RefSeq; XP_011529907.1; XM_011531605.1.
DR RefSeq; XP_011529908.1; XM_011531606.1.
DR RefSeq; XP_011529909.1; XM_011531607.2.
DR AlphaFoldDB; Q96MM3; -.
DR SMR; Q96MM3; -.
DR BioGRID; 126327; 18.
DR IntAct; Q96MM3; 5.
DR STRING; 9606.ENSP00000317686; -.
DR iPTMnet; Q96MM3; -.
DR PhosphoSitePlus; Q96MM3; -.
DR BioMuta; ZFP42; -.
DR DMDM; 296453032; -.
DR EPD; Q96MM3; -.
DR jPOST; Q96MM3; -.
DR MassIVE; Q96MM3; -.
DR MaxQB; Q96MM3; -.
DR PaxDb; Q96MM3; -.
DR PeptideAtlas; Q96MM3; -.
DR PRIDE; Q96MM3; -.
DR ProteomicsDB; 77373; -.
DR Antibodypedia; 17568; 385 antibodies from 31 providers.
DR DNASU; 132625; -.
DR Ensembl; ENST00000326866.5; ENSP00000317686.4; ENSG00000179059.10.
DR Ensembl; ENST00000509524.5; ENSP00000424662.1; ENSG00000179059.10.
DR Ensembl; ENST00000618147.1; ENSP00000483363.1; ENSG00000179059.10.
DR GeneID; 132625; -.
DR KEGG; hsa:132625; -.
DR MANE-Select; ENST00000326866.5; ENSP00000317686.4; NM_174900.5; NP_777560.2.
DR UCSC; uc003izh.2; human.
DR CTD; 132625; -.
DR DisGeNET; 132625; -.
DR GeneCards; ZFP42; -.
DR HGNC; HGNC:30949; ZFP42.
DR HPA; ENSG00000179059; Tissue enhanced (testis).
DR MIM; 614572; gene.
DR neXtProt; NX_Q96MM3; -.
DR OpenTargets; ENSG00000179059; -.
DR PharmGKB; PA142670530; -.
DR VEuPathDB; HostDB:ENSG00000179059; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000163257; -.
DR HOGENOM; CLU_085915_0_0_1; -.
DR InParanoid; Q96MM3; -.
DR OMA; PDCYIEC; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q96MM3; -.
DR TreeFam; TF106493; -.
DR PathwayCommons; Q96MM3; -.
DR SignaLink; Q96MM3; -.
DR SIGNOR; Q96MM3; -.
DR BioGRID-ORCS; 132625; 16 hits in 1094 CRISPR screens.
DR GeneWiki; Rex1; -.
DR GenomeRNAi; 132625; -.
DR Pharos; Q96MM3; Tbio.
DR PRO; PR:Q96MM3; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q96MM3; protein.
DR Bgee; ENSG00000179059; Expressed in buccal mucosa cell and 31 other tissues.
DR Genevisible; Q96MM3; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0031519; C:PcG protein complex; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0008585; P:female gonad development; IEP:UniProtKB.
DR GO; GO:0008584; P:male gonad development; IEP:UniProtKB.
DR GO; GO:0051321; P:meiotic cell cycle; NAS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 4.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..310
FT /note="Zinc finger protein 42 homolog"
FT /id="PRO_0000287289"
FT ZN_FING 188..212
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 217..239
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 245..269
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 275..299
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 231
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P22227"
FT CROSSLNK 233
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P22227"
FT CONFLICT 88
FT /note="E -> G (in Ref. 2; AAR07993 and 3; BAB71264)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 310 AA; 34802 MW; FBF4D7470DC37325 CRC64;
MSQQLKKRAK TRHQKGLGGR APSGAKPRQG KSSQDLQAEI EPVSAVWALC DGYVCYEPGP
QALGGDDFSD CYIECVIRGE FSQPILEEDS LFESLEYLKK GSEQQLSQKV FEASSLECSL
EYMKKGVKKE LPQKIVGENS LEYSEYMTGK KLPPGGIPGI DLSDPKQLAE FARKKPPINK
EYDSLSAIAC PQSGCTRKLR NRAALRKHLL IHGPRDHVCA ECGKAFVESS KLKRHFLVHT
GEKPFRCTFE GCGKRFSLDF NLRTHVRIHT GEKRFVCPFQ GCNRRFIQSN NLKAHILTHA
NTNKNEQEGK
