ZFP8_ARATH
ID ZFP8_ARATH Reviewed; 257 AA.
AC P93751; Q39267;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Zinc finger protein 8 {ECO:0000305};
GN Name=ZFP8 {ECO:0000303|PubMed:7599312}; OrderedLocusNames=At2g41940;
GN ORFNames=T6D20.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta; TISSUE=Leaf;
RX PubMed=7599312; DOI=10.1007/bf00020246;
RA Tague B.W., Goodman H.M.;
RT "Characterization of a family of Arabidopsis zinc finger protein cDNAs.";
RL Plant Mol. Biol. 28:267-279(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17507408; DOI=10.1242/dev.005017;
RA Gan Y., Liu C., Yu H., Broun P.;
RT "Integration of cytokinin and gibberellin signalling by Arabidopsis
RT transcription factors GIS, ZFP8 and GIS2 in the regulation of epidermal
RT cell fate.";
RL Development 134:2073-2081(2007).
RN [6]
RP FUNCTION.
RX PubMed=24808098; DOI=10.1104/pp.113.234294;
RA Joseph M.P., Papdi C., Kozma-Bognar L., Nagy I., Lopez-Carbonell M.,
RA Rigo G., Koncz C., Szabados L.;
RT "The Arabidopsis ZINC FINGER PROTEIN3 interferes with abscisic acid and
RT light signaling in seed germination and plant development.";
RL Plant Physiol. 165:1203-1220(2014).
CC -!- FUNCTION: Probable transcription factor required for the initiation of
CC inflorescence trichomes in response to gibberellin and cytokinin. Is
CC not involved in the regulation of trichome branching. Is functionally
CC equivalent to GIS2 (PubMed:17507408). Acts as negative regulator of
CC abscisic acid (ABA) signaling during germination and early seedling
CC development (PubMed:24808098). {ECO:0000269|PubMed:17507408,
CC ECO:0000269|PubMed:24808098}.
CC -!- INTERACTION:
CC P93751; O80748: B-box domain protein 26; NbExp=3; IntAct=EBI-15193377, EBI-15191535;
CC P93751; Q9S7W5: TCP13; NbExp=3; IntAct=EBI-15193377, EBI-4424877;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q39261}.
CC -!- TISSUE SPECIFICITY: Expressed in developing cauline leaves.
CC {ECO:0000269|PubMed:17507408}.
CC -!- INDUCTION: By gibberellin. {ECO:0000269|PubMed:17507408}.
CC -!- DISRUPTION PHENOTYPE: Reduced trichome production on cauline leaves,
CC stem branches and sepals. {ECO:0000269|PubMed:17507408}.
CC -!- MISCELLANEOUS: Seeds over-expressing ZFP8 show decreased sensitivity to
CC inhibition of germination by abscisic acid (ABA).
CC {ECO:0000269|PubMed:24808098}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA87304.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L39651; AAA87304.1; ALT_SEQ; mRNA.
DR EMBL; U90439; AAB63548.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10051.1; -; Genomic_DNA.
DR EMBL; AY039597; AAK62652.1; -; mRNA.
DR EMBL; AY056093; AAL06981.1; -; mRNA.
DR PIR; A84848; A84848.
DR PIR; S55888; S55888.
DR RefSeq; NP_181725.1; NM_129758.3.
DR AlphaFoldDB; P93751; -.
DR BioGRID; 4131; 32.
DR IntAct; P93751; 30.
DR STRING; 3702.AT2G41940.1; -.
DR PaxDb; P93751; -.
DR PRIDE; P93751; -.
DR ProteomicsDB; 242977; -.
DR EnsemblPlants; AT2G41940.1; AT2G41940.1; AT2G41940.
DR GeneID; 818794; -.
DR Gramene; AT2G41940.1; AT2G41940.1; AT2G41940.
DR KEGG; ath:AT2G41940; -.
DR Araport; AT2G41940; -.
DR TAIR; locus:2064579; AT2G41940.
DR eggNOG; ENOG502R1BM; Eukaryota.
DR HOGENOM; CLU_058544_1_0_1; -.
DR InParanoid; P93751; -.
DR OMA; PAFQTHE; -.
DR OrthoDB; 1384062at2759; -.
DR PhylomeDB; P93751; -.
DR PRO; PR:P93751; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P93751; baseline and differential.
DR Genevisible; P93751; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009788; P:negative regulation of abscisic acid-activated signaling pathway; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0010026; P:trichome differentiation; IMP:TAIR.
DR GO; GO:0010090; P:trichome morphogenesis; IEA:InterPro.
DR InterPro; IPR044291; GIS/GIS2/ZFP8.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR46547; PTHR46547; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW Abscisic acid signaling pathway; Cytokinin signaling pathway;
KW Developmental protein; Differentiation; Gibberellin signaling pathway;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..257
FT /note="Zinc finger protein 8"
FT /id="PRO_0000425723"
FT ZN_FING 95..117
FT /note="C2H2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 48..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 108..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 214..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..92
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 257 AA; 29571 MW; FE7A02F2CAD6B024 CRC64;
MDETNGRRET HDFMNVNVES FSQLPFIRRT PPKEKAAIIR LFGQELVGDN SDNLSAEPSD
HQTTTKNDES SENIKDKDKE KDKDKDKDNN NNRRFECHYC FRNFPTSQAL GGHQNAHKRE
RQHAKRGSMT SYLHHHQPHD PHHIYGFLNN HHHRHYPSWT TEARSYYGGG GHQTPSYYSR
NTLAPPSSNP PTINGSPLGL WRVPPSTSTN TIQGVYSSSP ASAFRSHEQE TNKEPNNWPY
RLMKPNVQDH VSLDLHL
