ZFPL1_HUMAN
ID ZFPL1_HUMAN Reviewed; 310 AA.
AC O95159; A8K7E9; O14616; Q9UID0;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Zinc finger protein-like 1;
DE AltName: Full=Zinc finger protein MCG4;
GN Name=ZFPL1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9253601; DOI=10.1101/gr.7.7.725;
RA Guru S.C., Agarwal S.K., Manickam P., Olufemi S.-E., Crabtree J.S.,
RA Weisemann J.M., Kester M.B., Kim Y.S., Wang Y., Emmert-Buck M.R.,
RA Liotta L.A., Spiegel A.M., Boguski M.S., Roe B.A., Collins F.S.,
RA Burns A.L., Marx S.J., Chandrasekharappa S.C.;
RT "A transcript map for the 2.8-Mb region containing the multiple endocrine
RT neoplasia type 1 locus.";
RL Genome Res. 7:725-735(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=9653652; DOI=10.1006/geno.1998.5307;
RA Hoeppener J.W.M., De Wit M.J., Simarro-Doorten A.Y., Roijers J.F.M.,
RA van Herrewaarden H.M.C., Lips C.J.M., Parente F., Quincey D., Gaudray P.,
RA Khodaei S., Weber G., Teh B., Farnebo F., Larsson C., Zhang C.X.,
RA Calender A., Pannett A.A.J., Forbes S.A., Bassett J.H.D., Thakker R.V.,
RA Lemmens I., Van de Ven W.J.M., Kas K.;
RT "A putative human zinc-finger gene (ZFPL1) on 11q13, highly conserved in
RT the mouse and expressed in exocrine pancreas.";
RL Genomics 50:251-259(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Silins G.U., Grimmond S., Hayward N.;
RT "Characterisation of a new human zinc-finger protein.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Neuroblastoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, PHOSPHORYLATION, INTERACTION WITH
RP GOLGA2, AND MUTAGENESIS OF CYS-27 AND CYS-53.
RX PubMed=18323775; DOI=10.1038/emboj.2008.40;
RA Chiu C.-F., Ghanekar Y., Frost L., Diao A., Morrison D., McKenzie E.,
RA Lowe M.;
RT "ZFPL1, a novel ring finger protein required for cis-Golgi integrity and
RT efficient ER-to-Golgi transport.";
RL EMBO J. 27:934-947(2008).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Required for cis-Golgi integrity and efficient ER to Golgi
CC transport. Involved in the maintenance of the integrity of the cis-
CC Golgi, possibly via its interaction with GOLGA2/GM130.
CC {ECO:0000269|PubMed:18323775}.
CC -!- SUBUNIT: Interacts with GOLGA2/GM130. {ECO:0000269|PubMed:18323775}.
CC -!- INTERACTION:
CC O95159; Q8IVF2-3: AHNAK2; NbExp=3; IntAct=EBI-718439, EBI-12078468;
CC O95159; Q13323: BIK; NbExp=3; IntAct=EBI-718439, EBI-700794;
CC O95159; Q8WZ55: BSND; NbExp=3; IntAct=EBI-718439, EBI-7996695;
CC O95159; P07766: CD3E; NbExp=3; IntAct=EBI-718439, EBI-1211297;
CC O95159; Q99675: CGRRF1; NbExp=3; IntAct=EBI-718439, EBI-2130213;
CC O95159; Q8IUN9: CLEC10A; NbExp=3; IntAct=EBI-718439, EBI-2873246;
CC O95159; Q9BXN2-6: CLEC7A; NbExp=3; IntAct=EBI-718439, EBI-11989440;
CC O95159; Q7Z7G2: CPLX4; NbExp=3; IntAct=EBI-718439, EBI-18013275;
CC O95159; Q96BA8: CREB3L1; NbExp=5; IntAct=EBI-718439, EBI-6942903;
CC O95159; Q53TN4: CYBRD1; NbExp=3; IntAct=EBI-718439, EBI-8637742;
CC O95159; Q15125: EBP; NbExp=3; IntAct=EBI-718439, EBI-3915253;
CC O95159; Q9GZR5: ELOVL4; NbExp=3; IntAct=EBI-718439, EBI-18535450;
CC O95159; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-718439, EBI-781551;
CC O95159; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-718439, EBI-18304435;
CC O95159; Q969F0: FATE1; NbExp=3; IntAct=EBI-718439, EBI-743099;
CC O95159; Q9Y680: FKBP7; NbExp=3; IntAct=EBI-718439, EBI-3918971;
CC O95159; Q14318: FKBP8; NbExp=3; IntAct=EBI-718439, EBI-724839;
CC O95159; Q8TBE3: FNDC9; NbExp=3; IntAct=EBI-718439, EBI-12142257;
CC O95159; A2A2Y4: FRMD3; NbExp=3; IntAct=EBI-718439, EBI-6911547;
CC O95159; Q5T7V8: GORAB; NbExp=3; IntAct=EBI-718439, EBI-3917143;
CC O95159; O15529: GPR42; NbExp=3; IntAct=EBI-718439, EBI-18076404;
CC O95159; P31937: HIBADH; NbExp=3; IntAct=EBI-718439, EBI-11427100;
CC O95159; Q8N6L0: KASH5; NbExp=3; IntAct=EBI-718439, EBI-749265;
CC O95159; P26715: KLRC1; NbExp=3; IntAct=EBI-718439, EBI-9018187;
CC O95159; Q5T700: LDLRAD1; NbExp=3; IntAct=EBI-718439, EBI-10173166;
CC O95159; A8MZ59: LEUTX; NbExp=3; IntAct=EBI-718439, EBI-17490413;
CC O95159; Q9H400: LIME1; NbExp=3; IntAct=EBI-718439, EBI-2830566;
CC O95159; Q9GZY8-5: MFF; NbExp=3; IntAct=EBI-718439, EBI-11956541;
CC O95159; Q5SR56: MFSD14B; NbExp=3; IntAct=EBI-718439, EBI-373355;
CC O95159; O14880: MGST3; NbExp=3; IntAct=EBI-718439, EBI-724754;
CC O95159; P30301: MIP; NbExp=3; IntAct=EBI-718439, EBI-8449636;
CC O95159; Q9HC36: MRM3; NbExp=3; IntAct=EBI-718439, EBI-1045440;
CC O95159; Q9Y676: MRPS18B; NbExp=3; IntAct=EBI-718439, EBI-750085;
CC O95159; Q96HJ5: MS4A3; NbExp=3; IntAct=EBI-718439, EBI-12806656;
CC O95159; Q96E29: MTERF3; NbExp=3; IntAct=EBI-718439, EBI-7825321;
CC O95159; O15173: PGRMC2; NbExp=3; IntAct=EBI-718439, EBI-1050125;
CC O95159; Q53GL0: PLEKHO1; NbExp=3; IntAct=EBI-718439, EBI-949945;
CC O95159; P15151: PVR; NbExp=3; IntAct=EBI-718439, EBI-3919694;
CC O95159; Q8NC24: RELL2; NbExp=3; IntAct=EBI-718439, EBI-10269209;
CC O95159; Q9NY72: SCN3B; NbExp=3; IntAct=EBI-718439, EBI-17247926;
CC O95159; Q8NHU3: SGMS2; NbExp=3; IntAct=EBI-718439, EBI-10977284;
CC O95159; Q3SXP7: SHISAL1; NbExp=3; IntAct=EBI-718439, EBI-18037857;
CC O95159; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-718439, EBI-18159983;
CC O95159; Q9BZV2: SLC19A3; NbExp=3; IntAct=EBI-718439, EBI-3923779;
CC O95159; Q8TBB6: SLC7A14; NbExp=3; IntAct=EBI-718439, EBI-5235586;
CC O95159; Q9BZL3: SMIM3; NbExp=5; IntAct=EBI-718439, EBI-741850;
CC O95159; Q8WWF3: SSMEM1; NbExp=3; IntAct=EBI-718439, EBI-17280858;
CC O95159; Q16623: STX1A; NbExp=3; IntAct=EBI-718439, EBI-712466;
CC O95159; P32856-2: STX2; NbExp=3; IntAct=EBI-718439, EBI-11956649;
CC O95159; Q12846: STX4; NbExp=3; IntAct=EBI-718439, EBI-744942;
CC O95159; Q96A49: SYAP1; NbExp=3; IntAct=EBI-718439, EBI-10770179;
CC O95159; P21579: SYT1; NbExp=3; IntAct=EBI-718439, EBI-524909;
CC O95159; Q8N6K0: TEX29; NbExp=3; IntAct=EBI-718439, EBI-19027521;
CC O95159; Q96DZ7: TM4SF19; NbExp=3; IntAct=EBI-718439, EBI-6448756;
CC O95159; Q9BVX2: TMEM106C; NbExp=3; IntAct=EBI-718439, EBI-2821497;
CC O95159; Q8IV31: TMEM139; NbExp=3; IntAct=EBI-718439, EBI-7238458;
CC O95159; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-718439, EBI-8638294;
CC O95159; Q96Q45-2: TMEM237; NbExp=3; IntAct=EBI-718439, EBI-10982110;
CC O95159; O15393-2: TMPRSS2; NbExp=3; IntAct=EBI-718439, EBI-12345267;
CC O95159; Q5T7W7: TSTD2; NbExp=3; IntAct=EBI-718439, EBI-8994397;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000269|PubMed:18323775}; Single-pass membrane protein
CC {ECO:0000269|PubMed:18323775}.
CC -!- TISSUE SPECIFICITY: Expressed strongly in the exocrine pancreas.
CC {ECO:0000269|PubMed:9653652}.
CC -!- DOMAIN: The B box-type and RING-type zinc fingers although degenerate
CC play a central role in function of the protein.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:18323775}.
CC -!- SIMILARITY: Belongs to the ZFPL1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB86533.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF001891; AAB86533.1; ALT_FRAME; mRNA.
DR EMBL; AF030291; AAF21634.1; -; mRNA.
DR EMBL; AF043611; AAD08644.1; -; mRNA.
DR EMBL; AK291964; BAF84653.1; -; mRNA.
DR EMBL; AP003068; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471076; EAW74344.1; -; Genomic_DNA.
DR EMBL; BC012530; AAH12530.1; -; mRNA.
DR EMBL; BC012814; AAH12814.1; -; mRNA.
DR CCDS; CCDS8092.1; -.
DR RefSeq; NP_006773.2; NM_006782.3.
DR AlphaFoldDB; O95159; -.
DR BioGRID; 113374; 457.
DR IntAct; O95159; 96.
DR MINT; O95159; -.
DR STRING; 9606.ENSP00000294258; -.
DR iPTMnet; O95159; -.
DR PhosphoSitePlus; O95159; -.
DR SwissPalm; O95159; -.
DR BioMuta; ZFPL1; -.
DR EPD; O95159; -.
DR jPOST; O95159; -.
DR MassIVE; O95159; -.
DR MaxQB; O95159; -.
DR PaxDb; O95159; -.
DR PeptideAtlas; O95159; -.
DR PRIDE; O95159; -.
DR ProteomicsDB; 50676; -.
DR Antibodypedia; 2657; 109 antibodies from 20 providers.
DR DNASU; 7542; -.
DR Ensembl; ENST00000294258.8; ENSP00000294258.3; ENSG00000162300.13.
DR GeneID; 7542; -.
DR KEGG; hsa:7542; -.
DR MANE-Select; ENST00000294258.8; ENSP00000294258.3; NM_006782.4; NP_006773.2.
DR UCSC; uc001ocq.2; human.
DR CTD; 7542; -.
DR DisGeNET; 7542; -.
DR GeneCards; ZFPL1; -.
DR HGNC; HGNC:12868; ZFPL1.
DR HPA; ENSG00000162300; Low tissue specificity.
DR MIM; 619397; gene.
DR neXtProt; NX_O95159; -.
DR OpenTargets; ENSG00000162300; -.
DR PharmGKB; PA37457; -.
DR VEuPathDB; HostDB:ENSG00000162300; -.
DR eggNOG; KOG3970; Eukaryota.
DR GeneTree; ENSGT00390000009753; -.
DR HOGENOM; CLU_075387_0_0_1; -.
DR InParanoid; O95159; -.
DR OMA; PNCALCN; -.
DR OrthoDB; 807302at2759; -.
DR PhylomeDB; O95159; -.
DR TreeFam; TF315090; -.
DR PathwayCommons; O95159; -.
DR SignaLink; O95159; -.
DR BioGRID-ORCS; 7542; 16 hits in 1116 CRISPR screens.
DR ChiTaRS; ZFPL1; human.
DR GenomeRNAi; 7542; -.
DR Pharos; O95159; Tbio.
DR PRO; PR:O95159; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; O95159; protein.
DR Bgee; ENSG00000162300; Expressed in mucosa of transverse colon and 188 other tissues.
DR ExpressionAtlas; O95159; baseline and differential.
DR Genevisible; O95159; HS.
DR GO; GO:0005794; C:Golgi apparatus; IDA:HPA.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; NAS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; NAS:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR039043; ZFPL1.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR12981; PTHR12981; 1.
PE 1: Evidence at protein level;
KW ER-Golgi transport; Golgi apparatus; Membrane; Metal-binding;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Zinc; Zinc-finger.
FT CHAIN 1..310
FT /note="Zinc finger protein-like 1"
FT /id="PRO_0000056318"
FT TOPO_DOM 1..266
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..310
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT ZN_FING 1..43
FT /note="B box-type; degenerate"
FT ZN_FING 53..101
FT /note="RING-type; degenerate"
FT REGION 145..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..169
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 211..231
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 218
FT /note="R -> Q (in dbSNP:rs35251366)"
FT /id="VAR_034472"
FT MUTAGEN 27
FT /note="C->A: Impairs the interaction with OLGA2/GM130 and
FT cis-Golgi assembly."
FT /evidence="ECO:0000269|PubMed:18323775"
FT MUTAGEN 53
FT /note="C->A: Impairs the interaction with OLGA2/GM130 and
FT cis-Golgi assembly."
FT /evidence="ECO:0000269|PubMed:18323775"
FT CONFLICT 182
FT /note="Q -> R (in Ref. 1; AAB86533 and 3; AAD08644)"
FT /evidence="ECO:0000305"
FT CONFLICT 246
FT /note="R -> Q (in Ref. 1; AAB86533)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 310 AA; 34114 MW; A12F1CA68A6E7F4F CRC64;
MGLCKCPKRK VTNLFCFEHR VNVCEHCLVA NHAKCIVQSY LQWLQDSDYN PNCRLCNIPL
ASRETTRLVC YDLFHWACLN ERAAQLPRNT APAGYQCPSC NGPIFPPTNL AGPVASALRE
KLATVNWARA GLGLPLIDEV VSPEPEPLNT SDFSDWSSFN ASSTPGPEEV DSASAAPAFY
SQAPRPPASP GRPEQHTVIH MGNPEPLTHA PRKVYDTRDD DRTPGLHGDC DDDKYRRRPA
LGWLARLLRS RAGSRKRPLT LLQRAGLLLL LGLLGFLALL ALMSRLGRAA ADSDPNLDPL
MNPHIRVGPS
