ZGLP1_MOUSE
ID ZGLP1_MOUSE Reviewed; 266 AA.
AC Q1WG82;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=GATA-type zinc finger protein 1 {ECO:0000305};
DE AltName: Full=GATA-like protein 1 {ECO:0000303|PubMed:16982049};
DE Short=GLP-1 {ECO:0000303|PubMed:16982049};
GN Name=Zglp1 {ECO:0000303|PubMed:16982049, ECO:0000303|PubMed:32054698};
GN Synonyms=Glp1 {ECO:0000303|PubMed:16982049};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=C57BL/6J;
RX PubMed=16982049; DOI=10.1016/j.ydbio.2006.07.048;
RA Li S., Lu M.M., Zhou D., Hammes S.R., Morrisey E.E.;
RT "GLP-1: a novel zinc finger protein required in somatic cells of the gonad
RT for germ cell development.";
RL Dev. Biol. 301:106-116(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=21123517; DOI=10.1530/rep-10-0376;
RA Strauss T.J., Castrillon D.H., Hammes S.R.;
RT "GATA-like protein-1 (GLP-1) is required for normal germ cell development
RT during embryonic oogenesis.";
RL Reproduction 141:173-181(2011).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=32054698; DOI=10.1126/science.aaw4115;
RA Nagaoka S.I., Nakaki F., Miyauchi H., Nosaka Y., Ohta H., Yabuta Y.,
RA Kurimoto K., Hayashi K., Nakamura T., Yamamoto T., Saitou M.;
RT "ZGLP1 is a determinant for the oogenic fate in mice.";
RL Science 0:0-0(2020).
CC -!- FUNCTION: Transcriptional regulator that plays a key role in germ cell
CC development (PubMed:16982049, PubMed:21123517, PubMed:32054698).
CC Determines the oogenic fate by activating key genes for the oogenic
CC program and meiotic prophase entry (PubMed:32054698). Acts downstream
CC of bone morphogenetic protein (BMP) by regulating expression of genes
CC required for the oogenic programs, which are repressed by Polycomb
CC activities in sexually uncommitted germ cells (PubMed:32054698).
CC Regulates expression of STRA8, a central downstream effector for the
CC meiotic program (PubMed:32054698). Acts independently of retinoic acid
CC (RA) (PubMed:32054698). In males, not required for germ-cell sex
CC determination, but required to allow the spermatogonia to efficiently
CC accomplish the meiotic prophase (PubMed:32054698).
CC {ECO:0000269|PubMed:16982049, ECO:0000269|PubMed:21123517,
CC ECO:0000269|PubMed:32054698}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16982049}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in adult testis and ovary
CC (PubMed:16982049). Expressed at high levels in the somatic cells of the
CC developing gonads, including Leydig cells in the testes and granulosa
CC cells in the ovaries (PubMed:16982049). {ECO:0000269|PubMed:16982049}.
CC -!- DEVELOPMENTAL STAGE: During female embryogenesis, specifically and
CC transiently expressed in germ cells expressing Ddx4: expression starts
CC at 12.0 dpc and decreases after 14.5 dpc, a key period for the sex
CC determination of germ cells (PubMed:21123517, PubMed:32054698). Not
CC expressed in somatic cells or males during embryogenesis
CC (PubMed:32054698). {ECO:0000269|PubMed:21123517,
CC ECO:0000269|PubMed:32054698}.
CC -!- INDUCTION: Up-regulated in response to bone morphogenetic proteins
CC (BMPs) signaling. {ECO:0000269|PubMed:32054698}.
CC -!- DISRUPTION PHENOTYPE: Mice are viable and normal; however, both males
CC and females are completely infertile (PubMed:16982049,
CC PubMed:32054698). In females, Zglp1 absence leads to a severe block in
CC germ cell development as early as 17.5 dpc (PubMed:16982049). Ovaries
CC at postnatal day (P) 8 and 6 weeks are highly atrophic with no ovarian
CC follicles (PubMed:32054698). Ovaries as early as 17.5 dpc contain a
CC drastically reduced number of cells expressing Ddx4 (PubMed:32054698).
CC In males, Zglp1 absence leads to defective sperm development with a
CC marked reduction in mature spermatids observed as early as postnatal
CC week 1 (PubMed:16982049). Male germ cells develop normally until P7
CC (PubMed:32054698). Subsequently, they display severe impairment in the
CC first wave of spermatogenesis: unlike in females, germ cells entered
CC into meiotic prophase, but fail to progress beyond the zygotene stage,
CC resulting in a large fraction of abnormal pachytene cells both at P15
CC and P20 (PubMed:32054698). {ECO:0000269|PubMed:16982049,
CC ECO:0000269|PubMed:32054698}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ286956; ABB89941.1; -; mRNA.
DR EMBL; AC163637; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS40549.1; -.
DR RefSeq; NP_001096638.1; NM_001103168.1.
DR AlphaFoldDB; Q1WG82; -.
DR STRING; 10090.ENSMUSP00000111157; -.
DR PhosphoSitePlus; Q1WG82; -.
DR PaxDb; Q1WG82; -.
DR PRIDE; Q1WG82; -.
DR ProteomicsDB; 275150; -.
DR Antibodypedia; 42858; 100 antibodies from 20 providers.
DR Ensembl; ENSMUST00000115494; ENSMUSP00000111157; ENSMUSG00000079681.
DR GeneID; 100009600; -.
DR KEGG; mmu:100009600; -.
DR UCSC; uc009veu.1; mouse.
DR CTD; 100125288; -.
DR MGI; MGI:3696042; Zglp1.
DR VEuPathDB; HostDB:ENSMUSG00000079681; -.
DR eggNOG; KOG1601; Eukaryota.
DR GeneTree; ENSGT00470000042444; -.
DR HOGENOM; CLU_088967_0_0_1; -.
DR InParanoid; Q1WG82; -.
DR OMA; GPCWELM; -.
DR OrthoDB; 1430172at2759; -.
DR PhylomeDB; Q1WG82; -.
DR BioGRID-ORCS; 100009600; 1 hit in 74 CRISPR screens.
DR PRO; PR:Q1WG82; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q1WG82; protein.
DR Bgee; ENSMUSG00000079681; Expressed in morula and 51 other tissues.
DR Genevisible; Q1WG82; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:ARUK-UCL.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0048599; P:oocyte development; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00320; GATA; 1.
DR PRINTS; PR00619; GATAZNFINGER.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; Differentiation; DNA-binding;
KW Metal-binding; Nucleus; Oogenesis; Reference proteome; Repressor;
KW Spermatogenesis; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..266
FT /note="GATA-type zinc finger protein 1"
FT /id="PRO_0000317558"
FT ZN_FING 197..221
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 106..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 106..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 171..185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 266 AA; 29107 MW; 40E266B6C889D587 CRC64;
MEAAQAGDLT RRQELLAPPC LDTESLRKSR PPALEPGALR CLTPNIRSLW PTCQDSVSTA
LPFLQEKEKG LPGSPSPATQ VLGSCWELMV IGMSDHLSMA RNPRGTQCPN LEISSATSPA
SLQRRPRKQL NPRMGIEKVD PRFKGVTLEF QIQPDSSLQI VPTYSLPGRS CSQKLPASPS
KALASPGSSE ALGPRRCASC RTQRTPLWRD AEDGTPLCNA CGIRYKKYGT RCSSCWLVPR
KSIQPKRLCG RCGMSQDPHL SPTQEL
