ID   ZGRF1_MOUSE             Reviewed;        1863 AA.
AC   Q0VGT4; E9Q2K6;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2013, sequence version 2.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Protein ZGRF1;
DE   AltName: Full=GRF-type zinc finger domain-containing protein 1;
GN   Name=Zgrf1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6J; TISSUE=Eye;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331 AND SER-445, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q0VGT4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q0VGT4-2; Sequence=VSP_053245, VSP_053246;
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DR   EMBL; AC113952; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC158308; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC085187; AAH85187.1; -; mRNA.
DR   CCDS; CCDS51066.1; -. [Q0VGT4-1]
DR   RefSeq; NP_932114.2; NM_197997.2. [Q0VGT4-1]
DR   AlphaFoldDB; Q0VGT4; -.
DR   SMR; Q0VGT4; -.
DR   STRING; 10090.ENSMUSP00000044432; -.
DR   iPTMnet; Q0VGT4; -.
DR   PhosphoSitePlus; Q0VGT4; -.
DR   EPD; Q0VGT4; -.
DR   MaxQB; Q0VGT4; -.
DR   PaxDb; Q0VGT4; -.
DR   PRIDE; Q0VGT4; -.
DR   ProteomicsDB; 275368; -. [Q0VGT4-1]
DR   ProteomicsDB; 275369; -. [Q0VGT4-2]
DR   Ensembl; ENSMUST00000043108; ENSMUSP00000044432; ENSMUSG00000051278. [Q0VGT4-1]
DR   Ensembl; ENSMUST00000195955; ENSMUSP00000142886; ENSMUSG00000051278. [Q0VGT4-2]
DR   Ensembl; ENSMUST00000196141; ENSMUSP00000143761; ENSMUSG00000051278. [Q0VGT4-1]
DR   GeneID; 71643; -.
DR   KEGG; mmu:71643; -.
DR   UCSC; uc008rhe.1; mouse. [Q0VGT4-2]
DR   UCSC; uc008rhh.2; mouse. [Q0VGT4-1]
DR   CTD; 55345; -.
DR   MGI; MGI:1918893; Zgrf1.
DR   VEuPathDB; HostDB:ENSMUSG00000051278; -.
DR   eggNOG; KOG1802; Eukaryota.
DR   GeneTree; ENSGT00940000162335; -.
DR   HOGENOM; CLU_236590_0_0_1; -.
DR   InParanoid; Q0VGT4; -.
DR   OMA; YWNSPRN; -.
DR   OrthoDB; 62494at2759; -.
DR   PhylomeDB; Q0VGT4; -.
DR   TreeFam; TF317543; -.
DR   BioGRID-ORCS; 71643; 1 hit in 74 CRISPR screens.
DR   ChiTaRS; Zgrf1; mouse.
DR   PRO; PR:Q0VGT4; -.
DR   Proteomes; UP000000589; Chromosome 3.
DR   RNAct; Q0VGT4; protein.
DR   Bgee; ENSMUSG00000051278; Expressed in manus and 145 other tissues.
DR   ExpressionAtlas; Q0VGT4; baseline and differential.
DR   Genevisible; Q0VGT4; MM.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0017108; F:5'-flap endonuclease activity; IBA:GO_Central.
DR   GO; GO:0004386; F:helicase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0071932; P:replication fork reversal; IBA:GO_Central.
DR   CDD; cd18808; SF1_C_Upf1; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR045055; DNA2/NAM7-like.
DR   InterPro; IPR041679; DNA2/NAM7-like_C.
DR   InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR   InterPro; IPR018838; DUF2439.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR010666; Znf_GRF.
DR   PANTHER; PTHR10887; PTHR10887; 1.
DR   Pfam; PF13086; AAA_11; 1.
DR   Pfam; PF13087; AAA_12; 1.
DR   Pfam; PF10382; DUF2439; 1.
DR   Pfam; PF06839; zf-GRF; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Membrane; Metal-binding; Phosphoprotein;
KW   Reference proteome; Transmembrane; Transmembrane helix; Zinc; Zinc-finger.
FT   CHAIN           1..1863
FT                   /note="Protein ZGRF1"
FT                   /id="PRO_0000286627"
FT   TRANSMEM        1705..1725
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ZN_FING         1109..1152
FT                   /note="GRF-type"
FT   REGION          78..110
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          132..196
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          300..349
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          460..496
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          524..545
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          610..664
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        92..107
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        158..196
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        303..326
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        473..489
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        529..545
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        621..635
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        646..660
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         331
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         445
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   VAR_SEQ         698..856
FT                   /note="ARKSPAIEVSRALLECPCEHRASQQPVEFQGHQVKGSATSGVMVRGHSLQRG
FT                   CTQFPDSIDYENFLTDTCVWTPGLPSTYGQTDFLQVISPEQKIPALSPAPTFSFNTRNE
FT                   DTVLELSEESLKTRTLPGLKTIGFQDSKNLQRFPFLSGASAPFATLPA -> GTQLFIL
FT                   QSHRDRNDEQVLSPISSSDISGQLLSTTQDHFECHELEESSTQISSPLFYPMGIKHPIY
FT                   RDSEAYISESKELGRIRSLPCDHFEVETTQRNQQSWATSKSSSELSEVINNMSLLKSQS
FT                   EHSTALGHLTILKKKHAFPHEVPHSRDPDVSPKG (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_053245"
FT   VAR_SEQ         857..1863
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_053246"
SQ   SEQUENCE   1863 AA;  205737 MW;  14D5C95C1FB6727F CRC64;
     MECQEFIVLY THQKMKKSKV WQDGVLKITH LGNKAILYDD KGACLESLFL KCLEVKPGDD
     LESERYLITV EEAKAVGSRA VEPDGSREAL ESGSRTLVSS SRSLGCQPSG LKRKATGFQR
     PYKMPKKVTI TENSEPAASL GDENPGPPGP RLLPTFSSTL PLFPTVGQKD LTPVSTDNQS
     PITFSNRERS DTPLSLPSSY FKINTNTLGK EDKLCFPVSS ETKHSDSLLA SEPMRRNGLD
     SHCPGVSQNV RSKAQILALL KSSSTNRKDL HGEIPGHFPK IEPQGCLNII SKPEEDYAET
     QSIGNLRCEQ QSENPTRTTS RWARYLPSQR SPPCSAADEN DTEDKPEAQE DVNIFNLSEL
     LQKKSELFET CSKKGELHSE DKPVDNTCQY WNQEDNLAPS FCKNSSVLVS CSSKENASLL
     SESDIQYSSK VPVNQHEKVW TREGSQGADA DAALEPEYRP VSPLPEIGHK QTEVEASLST
     SSRISDDIAD MGSKSNADRE DLKTVHKAVQ PFLEVSFNLS NFETSDTDEA SQEDSRLSQD
     SERWEKEAVL TDDSCVQKSC EDIGCREIVG KLPLLSSTDD EPKEALPADG TLLSEFCDRT
     CVGFNSGPHG DVKTGKALEG QCHSDTGSSL DSSLEWSEDV AGDSREDASQ SIQGNAINCG
     GVSPSKKPRG VNRSLYSPYL LTAVTDPAPE NSDLLSEARK SPAIEVSRAL LECPCEHRAS
     QQPVEFQGHQ VKGSATSGVM VRGHSLQRGC TQFPDSIDYE NFLTDTCVWT PGLPSTYGQT
     DFLQVISPEQ KIPALSPAPT FSFNTRNEDT VLELSEESLK TRTLPGLKTI GFQDSKNLQR
     FPFLSGASAP FATLPADDGP AVLDPCSFMI DDDAREPSGS SMLNLCEESG LSFDLGLEGQ
     GGTPGGVSLL PKSSTQSKWL KYQNPPQCNS TAPNRLASEV TEGLFAEAVS GLHFSHTSES
     ESSVDPVRLQ MIKGLLHQQQ QDLVSRKQAF SLTLNQTCKT QEHETVLGSS ASKNCRAKDL
     QEINNSDLCF PNGQKIISAY LPQRQVHIPA VFQSPAHYRQ VFTASIIEHL NILLFELAQR
     LYKALSKVDI SFYTSSKGET MRSGKNNSPS CHHNQPAKLV MVKKEGPNKG RLFYTCDKSK
     DNQCKFFKWL EEVTPGQLPQ NTSQSTMVFN DIKSIGSYLR SQKVPVYEEC QLLLRRGFDF
     QRKQCGKLKK LTTVNPDFYS ETKSKIYLKL SRRESSSVYS KGDLWVISKT LDFELDTFIA
     CSAFFGPSSV NEVELLPLKG YFPSNWPTNI TVHALLVCNA STELTTLQNI QDYFNPAALP
     LMPYLLAMSQ SATVSSKNIS KRKFIPPAIT SIKTKTELHL GATLQLAREL INVHRLNKDQ
     ATALIQIAQM MASQGSDEDA LEPFGHSLPI TVIHGVFGAG KSYLLAVVIL FLVELFEKCD
     SGTVGNARPW KVLVSSSTNV AVDRVLLGLL SLGFEKFIRV GSVRKIAKPV LPYSLHAGSD
     NESEQLKELN ALLKEELTPI ERVYVRKSIE QHKLGTNRVL LKQVRVVGVT CAACAFPCLN
     DLKFPVVVLD ECSQMTEPAS LLPIARFQCE KLILVGDPKQ LPPTIQGSDA AHENGLEQTL
     FDRLCLMGHK PVLLRTQYRC HPAISAIAND LFYEGSLVNG ISERERSPVL EWLPTLCFYN
     VTGAEQVERE NSFVNVAEAT FTLKLIQSLM ASGVESCMIG VITLYKSQMY KICNLLSAVD
     VGHPDVKAVQ VSTVDAFQGA EKEITILSCV RTRQVGFIDS EKRMNVALTR GRRHLLIVGS
     LSCLRKNRLW GRVIQHCEGR EDGLQHASQC EPQLDHLLKD YLEKQAEEKQ KKKEKEKSKD
     KSH