ZHANG_HUMAN
ID ZHANG_HUMAN Reviewed; 354 AA.
AC Q9NS37; B2R8Q9; Q0P5U9; Q52LT3;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=CREB/ATF bZIP transcription factor;
DE AltName: Full=Host cell factor-binding transcription factor Zhangfei;
DE Short=HCF-binding transcription factor Zhangfei;
GN Name=CREBZF; Synonyms=ZF;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAD28325.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH HCFC1, TISSUE
RP SPECIFICITY, MOTIF, AND MUTAGENESIS OF ASP-303; HIS-304 AND TYR-306.
RC TISSUE=Cervix carcinoma;
RX PubMed=10871379; DOI=10.1093/nar/28.12.2446;
RA Lu R., Misra V.;
RT "Zhangfei: a second cellular protein interacts with herpes simplex virus
RT accessory factor HCF in a manner similar to Luman and VP16.";
RL Nucleic Acids Res. 28:2446-2454(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-354.
RC TISSUE=Hippocampus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 74-354.
RC TISSUE=Bone marrow;
RA Zhang J.W., Zhang X., Ma Y.N.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000305}
RP FUNCTION AS A SUPPRESSOR OF CREB3 TRANSCRIPTIONAL ACTIVITY, INTERACTION
RP WITH CREB3 AND HCFC1, SUBCELLULAR LOCATION, AND MUTAGENESIS OF TYR-306.
RX PubMed=15705566; DOI=10.1074/jbc.m500728200;
RA Misra V., Rapin N., Akhova O., Bainbridge M., Korchinski P.;
RT "Zhangfei is a potent and specific inhibitor of the host cell factor-
RT binding transcription factor Luman.";
RL J. Biol. Chem. 280:15257-15266(2005).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Strongly activates transcription when bound to HCFC1.
CC Suppresses the expression of HSV proteins in cells infected with the
CC virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent
CC transcriptional activation by CREB3 and reduces the amount of CREB3 in
CC the cell. Able to down-regulate expression of some cellular genes in
CC CREBZF-expressing cells. {ECO:0000269|PubMed:10871379,
CC ECO:0000269|PubMed:15705566}.
CC -!- SUBUNIT: Interacts with HCFC1; the interaction inhibits CREB3
CC transcriptional activity (PubMed:10871379, PubMed:15705566). Interacts
CC with CREB3; the interaction occurs only in combination with HCFC1
CC (PubMed:15705566). {ECO:0000269|PubMed:10871379,
CC ECO:0000269|PubMed:15705566}.
CC -!- INTERACTION:
CC Q9NS37; P14621: ACYP2; NbExp=3; IntAct=EBI-632965, EBI-10198377;
CC Q9NS37; P18848: ATF4; NbExp=7; IntAct=EBI-632965, EBI-492498;
CC Q9NS37; O43889: CREB3; NbExp=3; IntAct=EBI-632965, EBI-625002;
CC Q9NS37; Q9NS37: CREBZF; NbExp=5; IntAct=EBI-632965, EBI-632965;
CC Q9NS37; Q8WYA6: CTNNBL1; NbExp=3; IntAct=EBI-632965, EBI-748128;
CC Q9NS37; P51610: HCFC1; NbExp=8; IntAct=EBI-632965, EBI-396176;
CC Q9NS37; P51610-4: HCFC1; NbExp=3; IntAct=EBI-632965, EBI-18150048;
CC Q9NS37; Q14494: NFE2L1; NbExp=3; IntAct=EBI-632965, EBI-2804436;
CC Q9NS37; Q16236: NFE2L2; NbExp=5; IntAct=EBI-632965, EBI-2007911;
CC Q9NS37; Q15311: RALBP1; NbExp=4; IntAct=EBI-632965, EBI-749285;
CC Q9NS37; Q96EB6: SIRT1; NbExp=3; IntAct=EBI-632965, EBI-1802965;
CC Q9NS37; O00268: TAF4; NbExp=2; IntAct=EBI-632965, EBI-1034261;
CC Q9NS37; Q5W5X9: TTC23; NbExp=3; IntAct=EBI-632965, EBI-6447954;
CC Q9NS37; Q5W5X9-3: TTC23; NbExp=3; IntAct=EBI-632965, EBI-9090990;
CC Q9NS37; P17861: XBP1; NbExp=4; IntAct=EBI-632965, EBI-6942961;
CC Q9NS37; P62509: Esrrg; Xeno; NbExp=3; IntAct=EBI-632965, EBI-5274019;
CC Q9NS37; P0C746: HBZ; Xeno; NbExp=3; IntAct=EBI-632965, EBI-10890294;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15705566}.
CC Note=Colocalizes in promyelocytic leukemia protein nuclear bodies (PML-
CC NB) with CREB3 and HCFC1.
CC -!- TISSUE SPECIFICITY: In adults, expressed most abundantly in heart,
CC liver and skeletal muscle, moderately abundant in kidney and pancreas,
CC and barely detectable in lung. In fetal tissues, expressed most
CC abundantly in kidney and very low amounts in heart, lung and liver.
CC {ECO:0000269|PubMed:10871379}.
CC -!- MISCELLANEOUS: Named 'Zhangfei' after a legendary Chinese warrior who
CC was contemporary with Luman in around 220 AD.
CC -!- SIMILARITY: Belongs to the bZIP family. ATF subfamily. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD28325.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAD28325.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAZ42189.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAG36256.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAG36256.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF039942; AAD28325.1; ALT_SEQ; mRNA.
DR EMBL; AP000642; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC060807; AAH60807.1; -; mRNA.
DR EMBL; BC093796; AAH93796.2; -; mRNA.
DR EMBL; BC093798; AAH93798.2; -; mRNA.
DR EMBL; AK313471; BAG36256.1; ALT_SEQ; mRNA.
DR EMBL; DQ128105; AAZ42189.1; ALT_INIT; mRNA.
DR CCDS; CCDS41697.1; -.
DR RefSeq; NP_001034707.1; NM_001039618.2.
DR RefSeq; XP_011543497.1; XM_011545195.2.
DR RefSeq; XP_016873577.1; XM_017018088.1.
DR RefSeq; XP_016873578.1; XM_017018089.1.
DR RefSeq; XP_016873579.1; XM_017018090.1.
DR RefSeq; XP_016873580.1; XM_017018091.1.
DR RefSeq; XP_016873581.1; XM_017018092.1.
DR AlphaFoldDB; Q9NS37; -.
DR SMR; Q9NS37; -.
DR BioGRID; 121817; 23.
DR ComplexPortal; CPX-6542; bZIP transcription factor complex, ATF4-CREBZF.
DR ComplexPortal; CPX-6601; bZIP transcription factor complex, ATF6B-CREBZF.
DR DIP; DIP-33934N; -.
DR ELM; Q9NS37; -.
DR IntAct; Q9NS37; 22.
DR MINT; Q9NS37; -.
DR STRING; 9606.ENSP00000433459; -.
DR iPTMnet; Q9NS37; -.
DR PhosphoSitePlus; Q9NS37; -.
DR BioMuta; CREBZF; -.
DR DMDM; 251757415; -.
DR EPD; Q9NS37; -.
DR jPOST; Q9NS37; -.
DR MassIVE; Q9NS37; -.
DR MaxQB; Q9NS37; -.
DR PaxDb; Q9NS37; -.
DR PeptideAtlas; Q9NS37; -.
DR PRIDE; Q9NS37; -.
DR ProteomicsDB; 82476; -.
DR Antibodypedia; 31388; 203 antibodies from 27 providers.
DR DNASU; 58487; -.
DR Ensembl; ENST00000490820.2; ENSP00000434281.1; ENSG00000137504.15.
DR Ensembl; ENST00000527447.2; ENSP00000433459.1; ENSG00000137504.15.
DR GeneID; 58487; -.
DR KEGG; hsa:58487; -.
DR MANE-Select; ENST00000527447.2; ENSP00000433459.1; NM_001039618.4; NP_001034707.1.
DR UCSC; uc001pas.3; human.
DR CTD; 58487; -.
DR DisGeNET; 58487; -.
DR GeneCards; CREBZF; -.
DR HGNC; HGNC:24905; CREBZF.
DR HPA; ENSG00000137504; Low tissue specificity.
DR MIM; 606444; gene.
DR neXtProt; NX_Q9NS37; -.
DR OpenTargets; ENSG00000137504; -.
DR PharmGKB; PA162382821; -.
DR VEuPathDB; HostDB:ENSG00000137504; -.
DR eggNOG; ENOG502S3I0; Eukaryota.
DR GeneTree; ENSGT00390000016589; -.
DR InParanoid; Q9NS37; -.
DR OMA; PEDMDFL; -.
DR PhylomeDB; Q9NS37; -.
DR TreeFam; TF336153; -.
DR PathwayCommons; Q9NS37; -.
DR SignaLink; Q9NS37; -.
DR BioGRID-ORCS; 58487; 19 hits in 1085 CRISPR screens.
DR ChiTaRS; CREBZF; human.
DR GenomeRNAi; 58487; -.
DR Pharos; Q9NS37; Tbio.
DR PRO; PR:Q9NS37; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9NS37; protein.
DR Bgee; ENSG00000137504; Expressed in sural nerve and 196 other tissues.
DR ExpressionAtlas; Q9NS37; baseline and differential.
DR Genevisible; Q9NS37; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005739; C:mitochondrion; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IPI:ComplexPortal.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0036500; P:ATF6-mediated unfolded protein response; IC:ComplexPortal.
DR GO; GO:0140467; P:integrated stress response signaling; IC:ComplexPortal.
DR GO; GO:0045814; P:negative regulation of gene expression, epigenetic; IEP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:ComplexPortal.
DR GO; GO:0051090; P:regulation of DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:GO_Central.
DR GO; GO:0009615; P:response to virus; IDA:UniProtKB.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR Pfam; PF00170; bZIP_1; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..354
FT /note="CREB/ATF bZIP transcription factor"
FT /id="PRO_0000076645"
FT DOMAIN 204..267
FT /note="bZIP"
FT REGION 1..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 113..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 219..226
FT /note="Basic motif"
FT /evidence="ECO:0000250"
FT REGION 232..267
FT /note="Leucine-zipper"
FT /evidence="ECO:0000250"
FT MOTIF 303..306
FT /note="HCFC1-binding motif (HBM)"
FT /evidence="ECO:0000269|PubMed:10871379"
FT COMPBIAS 8..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 50
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MUTAGEN 303
FT /note="D->A: Significantly reduced binding to HCFC1."
FT /evidence="ECO:0000269|PubMed:10871379"
FT MUTAGEN 304
FT /note="H->A: Significantly reduced binding to HCFC1."
FT /evidence="ECO:0000269|PubMed:10871379"
FT MUTAGEN 306
FT /note="Y->A: Does not interact with HCFC1 and is
FT inefficient at inhibiting CREB3 transcriptional activity.
FT Does not colocalize with CREB3 in promyelocytic leukemia
FT protein nuclear bodies (PML-NB)."
FT /evidence="ECO:0000269|PubMed:10871379,
FT ECO:0000269|PubMed:15705566"
FT CONFLICT 16
FT /note="S -> F (in Ref. 3; AAH60807)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 354 AA; 37134 MW; BE3AC77203216155 CRC64;
MRHSLTKLLA ASGSNSPTRS ESPEPAATCS LPSDLTRAAA GEEETAAAGS PGRKQQFGDE
GELEAGRGSR GGVAVRAPSP EEMEEEAIAS LPGEETEDMD FLSGLELADL LDPRQPDWHL
DPGLSSPGPL SSSGGGSDSG GLWRGDDDDE AAAAEMQRFS DLLQRLLNGI GGCSSSSDSG
SAEKRRRKSP GGGGGGGSGN DNNQAATKSP RKAAAAAARL NRLKKKEYVM GLESRVRGLA
AENQELRAEN RELGKRVQAL QEESRYLRAV LANETGLARL LSRLSGVGLR LTTSLFRDSP
AGDHDYALPV GKQKQDLLEE DDSAGGVCLH VDKDKVSVEF CSACARKASS SLKM
