ZHD1_ARATH
ID ZHD1_ARATH Reviewed; 279 AA.
AC Q9FKP8;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=Zinc-finger homeodomain protein 1;
DE Short=AtZHD1;
DE AltName: Full=Homeobox protein 25;
DE Short=AtHB-25;
DE AltName: Full=Zinc finger homeodomain transcription factor 2;
GN Name=ZHD1; Synonyms=HB25, ZFHD2; OrderedLocusNames=At5g65410;
GN ORFNames=MNA5.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP INTERACTION WITH ZHD2; ZHD3; ZHD4; ZHD5; ZHD6; ZHD7; ZHD8; ZHD9; ZHD10 AND
RP ZHD11, TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=16428600; DOI=10.1104/pp.105.070565;
RA Tan Q.K., Irish V.F.;
RT "The Arabidopsis zinc finger-homeodomain genes encode proteins with unique
RT biochemical properties that are coordinately expressed during floral
RT development.";
RL Plant Physiol. 140:1095-1108(2006).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18713354; DOI=10.1111/j.1744-7909.2008.00681.x;
RA Hu W., dePamphilis C.W., Ma H.;
RT "Phylogenetic analysis of the plant-specific zinc finger-homeobox and mini
RT zinc finger gene families.";
RL J. Integr. Plant Biol. 50:1031-1045(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [7]
RP INTERACTION WITH MIF1 AND MIF2, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21059647; DOI=10.1074/jbc.m110.167692;
RA Hong S.-Y., Kim O.-K., Kim S.-G., Yang M.-S., Park C.-M.;
RT "Nuclear import and DNA binding of the ZHD5 transcription factor is
RT modulated by a competitive peptide inhibitor in Arabidopsis.";
RL J. Biol. Chem. 286:1659-1668(2011).
RN [8]
RP STRUCTURE BY NMR OF 182-248.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of homeobox domain of Arabidopsis thaliana zinc finger
RT homeobox family protein.";
RL Submitted (NOV-2004) to the PDB data bank.
CC -!- FUNCTION: Putative transcription factor.
CC -!- SUBUNIT: Homo- and heterodimer with other ZFHD proteins (By
CC similarity). Interacts with MIF1 and MIF2; these interactions prevent
CC nuclear localization and DNA-binding to inhibit transcription
CC regulation activity. Binds to ZHD2, ZHD3, ZHD4, ZHD5, ZHD6, ZHD7, ZHD8,
CC ZHD9, ZHD10 and ZHD11. {ECO:0000250, ECO:0000269|PubMed:16428600,
CC ECO:0000269|PubMed:21059647}.
CC -!- INTERACTION:
CC Q9FKP8; Q9FIW9: ZHD10; NbExp=3; IntAct=EBI-766685, EBI-1806298;
CC Q9FKP8; Q9LXG0: ZHD8; NbExp=4; IntAct=EBI-766685, EBI-1806405;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. Note=Interactions with MIF
CC proteins prevent nuclear subcellular location and leads to a scattered
CC repartition throughout the cytoplasm.
CC -!- TISSUE SPECIFICITY: Mostly expressed in flowers and inflorescence.
CC {ECO:0000269|PubMed:16428600}.
CC -!- DOMAIN: The homeodomain differs form the typical one by having namely 4
CC instead of 3 extra amino acids inserted in the loop between helix 1 and
CC helix 2.
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DR EMBL; AB011479; BAB11563.1; -; Genomic_DNA.
DR EMBL; CP002688; AED98048.1; -; Genomic_DNA.
DR EMBL; BT002385; AAO00745.1; -; mRNA.
DR EMBL; BT006304; AAP13412.1; -; mRNA.
DR RefSeq; NP_201344.1; NM_125939.3.
DR PDB; 1WH5; NMR; -; A=182-248.
DR PDBsum; 1WH5; -.
DR AlphaFoldDB; Q9FKP8; -.
DR BMRB; Q9FKP8; -.
DR SMR; Q9FKP8; -.
DR BioGRID; 21908; 14.
DR IntAct; Q9FKP8; 13.
DR STRING; 3702.AT5G65410.1; -.
DR iPTMnet; Q9FKP8; -.
DR PaxDb; Q9FKP8; -.
DR PRIDE; Q9FKP8; -.
DR ProteomicsDB; 232322; -.
DR EnsemblPlants; AT5G65410.1; AT5G65410.1; AT5G65410.
DR GeneID; 836666; -.
DR Gramene; AT5G65410.1; AT5G65410.1; AT5G65410.
DR KEGG; ath:AT5G65410; -.
DR Araport; AT5G65410; -.
DR TAIR; locus:2168240; AT5G65410.
DR eggNOG; ENOG502QRG0; Eukaryota.
DR HOGENOM; CLU_039237_3_1_1; -.
DR InParanoid; Q9FKP8; -.
DR OMA; SIGWRIQ; -.
DR OrthoDB; 1229084at2759; -.
DR PhylomeDB; Q9FKP8; -.
DR EvolutionaryTrace; Q9FKP8; -.
DR PRO; PR:Q9FKP8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FKP8; baseline and differential.
DR Genevisible; Q9FKP8; AT.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010371; P:regulation of gibberellin biosynthetic process; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0010431; P:seed maturation; IMP:TAIR.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR006455; Homeodomain_ZF_HD.
DR InterPro; IPR006456; ZF_HD_homeobox_Cys/His_dimer.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF04770; ZF-HD_dimer; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR TIGRFAMs; TIGR01565; homeo_ZF_HD; 1.
DR TIGRFAMs; TIGR01566; ZF_HD_prot_N; 1.
DR PROSITE; PS51523; ZF_HD_DIMER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Homeobox; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..279
FT /note="Zinc-finger homeodomain protein 1"
FT /id="PRO_0000056847"
FT ZN_FING 75..124
FT /note="ZF-HD dimerization-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00856"
FT DNA_BIND 191..254
FT /note="Homeobox; atypical"
FT REGION 1..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 128..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 245..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..30
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..58
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 131..168
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 258..272
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 243
FT /note="Required for DNA-binding"
FT /evidence="ECO:0000250"
FT HELIX 200..213
FT /evidence="ECO:0007829|PDB:1WH5"
FT TURN 219..221
FT /evidence="ECO:0007829|PDB:1WH5"
FT HELIX 222..231
FT /evidence="ECO:0007829|PDB:1WH5"
FT HELIX 236..246
FT /evidence="ECO:0007829|PDB:1WH5"
SQ SEQUENCE 279 AA; 31098 MW; B9809820DD45B780 CRC64;
MEFEDNNNNN DEEQEEDMNL HEEEEDDDAV YDSPPLSRVL PKASTESHET TGTTSTGGGG
GFMVVHGGGG SRFRFRECLK NQAVNIGGHA VDGCGEFMPA GIEGTIDALK CAACGCHRNF
HRKELPYFHH APPQHQPPPP PPGFYRLPAP VSYRPPPSQA PPLQLALPPP QRERSEDPME
TSSAEAGGGI RKRHRTKFTA EQKERMLALA ERIGWRIQRQ DDEVIQRFCQ ETGVPRQVLK
VWLHNNKHTL GKSPSPLHHH QAPPPPPPQS SFHHEQDQP